Dissemination of carbapenem-resistant  Klebsiella pneumoniae  harboring KPC-carrying plasmid  p KPC_P16, a  p KPC_LK30 variant, in northern Taiwan

Chen, Jiann-Yuan; Liou, Ming-Li; Kuo, Han-Yueh; Lu, Chia-Wei; Lai, Yicheng; Lin, Yun-Yu; Chen, Changhua

doi:10.1016/j.diagmicrobio.2018.02.014

Cited by 5 publications

(1 citation statement)

References 23 publications

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“…Interestingly, those strains were mostly originated in China and seemed to be associated with K. pneumoniae ST11 type. 4,19,54,[63][64][65][66][67][68][69] These data implied that plasmids carrying this genetic background have been confined to K. pneumoniae ST11 so far and circulated since 2010 in mainland China. This resistant genetic background usually integrated into a fosA3-positive IncR-IncFII plasmid which was composed of three genetically and physically distinct modules: a pHN7A8-derived module which contains fosfomycin resistance gene fosA3 and IncFII replicon, a pKPC-LK30-derived core module which harbors bla KPC-2 gene and IncR replicon, and another 10 kb module.…”

Section: Discussionmentioning

confidence: 91%

Tandem Repeat of blaNDM-1 and Clonal Dissemination of a fosA3 and blaKPC-2 Co-Carrying IncR-F33: A–: B– Plasmid in Klebsiella pneumoniae Isolates Collected in a Southwest Hospital in China, 2010–2013

Hu¹,

Zhang²,

Shen³

et al. 2022

IDR

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Introduction Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been widespread in coastal cities of eastern China since 2009. However, how CRKP spreads and evolves in southwest China is unclear. Aim We investigated the genetic characteristics and dissemination mechanisms of carbapenemase genes in forty-one non-repetitive CRKP isolates collected from a southwest hospital, Kunming, Yunnan, during 2010–2013. Methodology Drug susceptibilities were analyzed by using VITEK 2 compact system. Genetic relationships were ascertained based on multilocus sequence typing (MLST) and Pulsed-field gel electrophoresis (PFGE) analysis. Genetic backgrounds of bla KPC-2 and bla NDM-1 were revealed by DNA walking and high-throughput sequencing. Results All isolates were highly resistant to common antibiotics except for tigecycline. In total, 34 bla KPC-2 , 3 bla NDM-1 , 1 bla IMP-4 and 3 bla IMP-26 genes were identified and KP67 plasmid 1 co-harbored bla NDM-1 and bla IMP-26 . Five sequence types, namely ST11, ST290, ST340, ST395 and ST437, were recognized by MLST. Surprisingly, bla KPC-2 was only detected in ST11 strains. We described a clonal dissemination of fosA3 -positive IncR-IncF33:A-:B- multireplicon plasmid carrying the gene cassettes IS 26 -ΔTn 3 -IS Kpn27-bla KPC-2 -ΔIS Kpn6-korC-klcA -Δ repB -Tn 1721 in all ST11 isolates. Three bla NDM-1 positive isolates belonged to three different ST types and their bla NDM-1 genetic backgrounds were also distinct. Interestingly, the flanking regions of bla NDM-1 in KP67 and KP72 were duplicated into one to five copies in a form of tandem repeat by the transposition of IS 91 like element. The bla NDM-1 of KP82 was carried on a common IncX3 plasmid. Conclusion This study described the early epidemiological characteristics of bla KPC-2 / bla NDM-1 -carrying CRKP, and reported a new tandem repeat pattern of bla NDM-1 cluster in Yunnan. These findings extend our knowledge on the carbapenemase gene evolutions.

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