Dissemination of nim-class genes, encoding nitroimidazole resistance, among different species of Gram-negative anaerobic bacteria isolated in Athens, Greece

Katsandri, Anastasia; Avlamis, Athina; Pantazatou, Angeliki; Houhoula, Dimitra; Papaparaskevas, Joseph

doi:10.1093/jac/dkl285

Cited by 27 publications

(20 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, none of the known IS elements that have been associated with nim genes were detected upstream of nimI. These findings are in accordance with those of previous studies that have reported that nim-positive strains may be metronidazole resistant in the absence of known activating IS elements (14,20,22). This suggests that, as for other nim genes, the expression of nimI may be modulated by mechanisms other than those related to the presence of IS elements, although it cannot be ruled out that new IS elements that are not recognized by the primers used may be involved.…”

Section: Discussionsupporting

confidence: 91%

“…(20,23,27), nim-positive Prevotella strains have rarely been found. Lubbe et al (23) detected one nimA-positive strain (Prevotella bivia) among seven strains screened, while Katsandri et al (20) recovered one nimC-positive strain (Prevotella oralis) and two nimE-positive isolates (P. oralis, Prevotella buccalis) from among 57 isolates tested.…”

Section: Discussionmentioning

confidence: 99%

“…have only rarely been investigated for the presence of nim genes in studies concerning metronidazole susceptibility (20,23,27). This led us to investigate a large panel of clinical Prevotella strains that belong to different species and that were isolated from patients hospitalized in two French tertiary-care teaching hospitals for the presence of nim genes and for the type of nim genes that they carry.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Metronidazole Resistance in Prevotella spp. and Description of a New nim Gene in Prevotella baroniae

Alauzet

Mory

Teyssier

et al. 2010

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Nonduplicate clinical isolates of Prevotella spp. recovered from patients hospitalized between 2003 and 2006in two French tertiary-care teaching hospitals were investigated for their susceptibility to metronidazole and the presence of nim genes. Of the 188 strains tested, 3 isolates displayed reduced susceptibility to metronidazole after 48 h of incubation, while 27 additional isolates exhibited heterogeneous resistance after prolonged incubation; all 30 of the isolates were nim negative. Among the remaining 158 isolates, 7 nim-positive isolates were detected. All of these strains were identified as Prevotella baroniae by 16S rRNA gene sequence analysis and contained a new nim gene, named nimI, as determined by DNA sequence analysis. Chromosomal localization of this single-copy gene was demonstrated in all clinical isolates as well as in type strain P. baroniae DSM 16972 by using Southern hybridization. No known associated insertion sequence elements were detected upstream of the nimI gene in any of the nim-positive strains by PCR mapping. After prolonged exposure to metronidazole, stable resistant subpopulations could be selected in nimI-positive Prevotella isolates (n ‫؍‬ 6) as well as in nim-negative Prevotella isolates (n ‫؍‬ 6), irrespective of their initial susceptibility to this antibiotic. This study is the first description of a new nitroimidazole resistance gene in P. baroniae which seems to be silent and which might be intrinsic in this species. Moreover, our findings highlight the fact that high-level resistance to metronidazole may be easily induced in both nim-positive and nim-negative Prevotella sp. strains.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Metronidazole Resistance in Prevotella spp. and Description of a New nim Gene in Prevotella baroniae

Alauzet

Mory

Teyssier

et al. 2010

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…In our case, MIC 90 values for tigecycline ranged from 0.125 to 1 g/ml, well below the 4 to 8 g/ml values reported by other authors, and unlike Betriu et al (3) and Grisold et al (11), no resistance of any kind was found. It is also important to highlight that not a single metronidazoleresistant isolate was found in Argentina, which is different from the situation in European countries and the United States (9,14,17,24,27).…”

Section: Discussionmentioning

confidence: 78%

“…he increasing resistance to antimicrobial agents among anaerobic bacteria has become a global problem in the past 2 decades, particularly within the species that make up the Bacteroides fragilis group, the most frequently isolated species in clinical infections, in which resistance to metronidazole, carbapenems, and ␤-lactam-␤-lactamase inhibitor combinations has been reported (14)(15)(16)29).…”

mentioning

confidence: 99%

First National Survey of Antibiotic Susceptibility of the Bacteroides fragilis Group: Emerging Resistance to Carbapenems in Argentina

Fernández-Canigia¹,

Litterio²,

Legaria³

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The antibiotic susceptibility rates of 363 clinical Bacteroides fragilis group isolates collected from 17 centers in Argentina during the period from 2006 to 2009 were as follows: piperacillin-tazobactam, 99%; ampicillin-sulbactam, 92%; cefoxitin, 72%; tigecycline, 100%; moxifloxacin, 91%; and clindamycin, 52%. No metronidazole resistance was detected in these isolates during this time period. Resistance to imipenem, doripenem, and ertapenem was observed in 1.1%, 1.6%, and 2.3% of B. fragilis group strains, respectively. B. fragilis species showed a resistance profile of 1.5% to imipenem, 1.9% to doripenem, and 2.4% to ertapenem. This is the first report of carbapenem resistance in Argentina. The cfiA gene was present in 8 out of 23 isolates, all of them belonging to the B. fragilis species and displaying reduced susceptibility or resistance to carbapenems (MICs > 4 g/ml). Three out of eight cfiA-positive isolates were fully resistant to carbapenems, while 5 out of 8 isolates showed low-level resistance (MICs, 4 to 8 g/ml). The inhibition by EDTA was a good predictor of the presence of metallo-␤-lactamases in the fully resistant B. fragilis strains, but discrepant results were observed for low-level resistant isolates. B. fragilis was more susceptible to antimicrobial agents than other Bacteroides species. Bacteroides vulgatus species was the most resistant to ampicillin-sulbactam and piperacillin-tazobactam, and B. thetaiotaomicron/ovatus strains showed the highest level of resistance to carbapenems, with an unknown resistance mechanism. B. vulgatus and the uncommon non-Bacteroides fragilis species were the most resistant to moxifloxacin, showing an overall resistance rate of 15.1%.

show abstract

Scarce detection of mobile erm genes associated with tetQ in Bacteroides and Parabacteroides from Costa Rica

Quesada-Gómez¹,

Rodríguez-Cavallini²,

Rodríguez³

2013

Anaerobe

View full text Add to dashboard Cite

Dissemination of nim-class genes, encoding nitroimidazole resistance, among different species of Gram-negative anaerobic bacteria isolated in Athens, Greece

Cited by 27 publications

References 5 publications

Metronidazole Resistance in Prevotella spp. and Description of a New nim Gene in Prevotella baroniae

Metronidazole Resistance in Prevotella spp. and Description of a New nim Gene in Prevotella baroniae

First National Survey of Antibiotic Susceptibility of the Bacteroides fragilis Group: Emerging Resistance to Carbapenems in Argentina

Scarce detection of mobile erm genes associated with tetQ in Bacteroides and Parabacteroides from Costa Rica

Contact Info

Product

Resources

About