Dissemination of the Fosfomycin Resistance Gene
            <i>fosA3</i>
            with CTX-M β-Lactamase Genes and
            <i>rmtB</i>
            Carried on IncFII Plasmids among Escherichia coli Isolates from Pets in China

Hou, Jianxia; Huang, Xianhui; Deng, Yuting; He, Liang-Ying; Yang, Tong; Zeng, Zhenling; Zhang-liu, Chen; Liu, Jianhua

doi:10.1128/aac.05104-11

Cited by 134 publications

(153 citation statements)

References 26 publications

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“…This indicated that both horizontal gene transfer and clonal spread were responsible for the dissemination of the rmtB gene in the E. coli strains in this study. The prevalence of plasmids carrying rmtB has frequently been observed to be associated with the incompatibility group F (IncF) in humans and other animals in China (Yu et al, 2010;Hou et al, 2012), and with IncA/ C in humans and IncF in companion animals in Korea (Kang et al, 2008;So et al, 2012). However, all rmtB-carrying plasmids in the present study belonged to IncI1.…”

Section: Transfer Of Aminoglycoside Resistance and Plasmid Replicon Tcontrasting

confidence: 50%

“…The rmtB gene is located between the Tn2 transposon and insertion elements such as IS26, ISCR1 and ISCR3 (Doi et al, 2008;Du et al, 2009). Recently, the rmtB gene has often been found to coexist with bla CTX-M and fosA3 (Hou et al, 2012;Lee et al, 2012;Sun et al, 2012;Pan et al, 2014), which could pose a threat to animal husbandry and public health. The dissemination of plasmids carrying rmtB has frequently been observed to be associated with the IncF and IncA/C incompatibility groups (Kang et al, 2008;Yu et al, 2010;Hou et al, 2012), whereas IncI1 plasmids harbouring rmtB are scarce.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Cheng

et al. 2016

Journal of Medical Microbiology

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The rmtB gene, one of the 16S rRNA methylase genes whose products confer high-level resistance to aminoglycosides, is most prevalent among Enterobacteriaceae strains. In this study, eight non-duplicate rmtB-carrying avian Escherichia coli strains from a farm in China were isolated and characterized, and further examined by phylogenetic grouping, conjugation experiments and PCR-based replicon typing. In addition, the genetic environment of rmtB was investigated by cloning and sequencing. Six rmtB-carrying E. coli were identified as phylogroup A, sequence type (ST) 156 (A-ST156), with two assigned to D-ST117; however, all of them carried the same IncI1 ST136 plasmid. The genetic environment of the rmtB gene in these eight plasmids was the same, as shown by PCR mapping. A multidrug-resistant region carrying bla TEM-1 , rmtB, a class 1 integron cassette array (intI1-dfrA12-orfF-aadA2-qacED1-sul1) and aacC2 was characterized on the conjugative IncI1 ST136 plasmid. Co-location of the rmtB gene with a class 1 integron cassette array and aacC2 on the conjugative plasmid will facilitate its maintenance and dissemination.

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Section: Transfer Of Aminoglycoside Resistance and Plasmid Replicon Tcontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Cheng

et al. 2016

Journal of Medical Microbiology

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“…In China, plasmid-mediated fosfomycin resistance has been frequently detected among CTX-M-producing isolates in animals (10,34). It was unexpected, since the use of fosfomycin in foodproducing animals is not approved in China.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

Xie

Lin

Chen

et al. 2016

Antimicrob Agents Chemother

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A total of 55 cefotaxime-resistant Escherichia coli isolates were obtained from retail meat products purchased in Shenzhen, China, during the period November 2012 to May 2013. Thirty-seven of these 55 isolates were found to harbor a bla CTX-M gene, with the bla CTX-M-1 group being the most common type. bla CMY-2 was detected in 16 isolates, alone or in combination with other extended-spectrum ␤-lactamase (ESBL) determinants. Importantly, the fosA3 gene, which encodes fosfomycin resistance, was detected in 12 isolates, with several being found to reside in the conjugative plasmid that harbored the bla CTX-M gene. The insertion sequence IS26 was observed upstream of some of the bla CTX-M-55 and fosA3 genes. Conjugation experiments showed that bla CTX-M genes from 15 isolates were transferrable, with Inc I1 and Inc FII being the most prevalent replicons. High clonal diversity was observed among the bla CTX-M producers, suggesting that horizontal transfer of the bla CTX-M genes among E. coli strains in retail meats is a common event and that such strains may constitute an important reservoir of bla CTX-M genes, which may be readily disseminated to other potential human pathogens.

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“…Several studies have reported the presence of fosfomycin resistance genes, along with various ␤-lactamases, including the AmpC-like and extendedspectrum ␤-lactamases (6,10). In this study, we characterized a newly identified fosA7 gene that confers resistance to fosfomycin in Salmonella Heidelberg strains isolated from broiler chickens.…”

mentioning

confidence: 99%

First Detection of a Fosfomycin Resistance Gene, fosA7 , in Salmonella enterica Serovar Heidelberg Isolated from Broiler Chickens

Rehman

Persaud-Lachhman

et al. 2017

Antimicrob Agents Chemother

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We previously described Salmonella enterica serovar Heidelberg isolates harboring a chromosomal gene cluster similar to the glutathione S-transferase gene, a putative fosA gene conferring resistance to fosfomycin. Here, we show that this new gene, named fosA7, confers resistance to fosfomycin. The introduction of fosA7 into the fosfomycin-susceptible Salmonella enterica serovar Enteritidis resulted in a substantial increase in the fosfomycin MIC. This finding increases the awareness of antibiotic resistance in Salmonella Heidelberg from broilers as related to the food safety and public health.

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Dissemination of the Fosfomycin Resistance Gene fosA3 with CTX-M β-Lactamase Genes and rmtB Carried on IncFII Plasmids among Escherichia coli Isolates from Pets in China

Cited by 134 publications

References 26 publications

Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

First Detection of a Fosfomycin Resistance Gene, fosA7 , in Salmonella enterica Serovar Heidelberg Isolated from Broiler Chickens

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Dissemination of the Fosfomycin Resistance Gene fosA3 with CTX-M β-Lactamase Genes and rmtB Carried on IncFII Plasmids among Escherichia coli Isolates from Pets in China

Cited by 134 publications

References 26 publications

Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla CTX-M and fosA3 Genes

First Detection of a Fosfomycin Resistance Gene, fosA7 , in Salmonella enterica Serovar Heidelberg Isolated from Broiler Chickens

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Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes