Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Fu, Tong; Du, Xiang-Dang; Cheng, Peipei; Li, Xiaoran; Zhao, Xin-Fang; Pan, Yongchu

doi:10.1099/jmm.0.000240

Cited by 4 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, this sequence type has been associated with the global carriage of ESBL-producing E. coli [13,34,48]. E. coli ST156 was identified in fish [50], canines, felines, horses [51], chickens, and other avian species [52,53]. Lastly, this study is the first demonstration of ST1380 E. coli being isolated from equines, the sequence type that was previously isolated from swine and bovine species [54,55].…”

Section: Discussionmentioning

confidence: 55%

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

View full text Add to dashboard Cite

Simple Summary: The spread and development of extended spectrum beta-lactamase (ESBL)-mediated antimicrobial resistance is a significant concern in healthcare with impacts to animal and public health alike. While the occurrence of the ESBL phenotype in Escherichia coli has been investigated in depth by numerous studies, there is still a lack of information regarding ESBL-producing bacterial isolates from clinical specimens of equine origin. In this study, we investigated the incidence of ESBL-producing E. coli in hospitalized horses. Overall, 207 E. coli isolates were analyzed for their antimicrobial susceptibility and 13 ESBL-producing E. coli isolates were genotypically characterized. Seven out of the 13 E. coli isolates were found to harbor the resistance genes bla CTX-M-1 or bla SHV-1 and a novel beta-lactamase TEM gene variant, bla TEM-233 was discovered. Furthermore, despite being phenotypically susceptible to tested carbapenems, 1 out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 . The latter is an alarming finding because the presence of carbapenemase resistance genes in equine pathogens is extremely rare. In conclusion, equines can be reservoirs for ESBL-producing Enterobacteriaceae, and further investigation into this species group is necessary to understand their impact in the spread and development of antibiotic resistance genes.Abstract: Escherichia coli isolates were recovered from clinical specimens of equine patients admitted to the Texas A&M Veterinary Medical Teaching Hospital over a five-year period. Ceftiofur resistance was used as a marker for potential extended-spectrum beta-lactamase (ESBL)-activity, and of the 48 ceftiofur-resistant E. coli isolates, 27.08% (n = 13) were phenotypically ESBL-positive. Conventional PCR analysis followed by the large-scale bla Finder multiplex PCR detected the ESBL genes, CTX-M-1 and SHV, in seven out of the 13 isolates. Moreover, beta-lactamase genes of TEM-1-type, BER-type (AmpC), and OXA-type were also identified. Sequencing of these genes resulted in identification of a novel TEM-1-type gene, called bla TEM-233 , and a study is currently underway to determine if this gene confers the ESBL phenotype. Furthermore, this report is the first to have found E. coli ST1308 in horses. This subtype, which has been reported in other herbivores, harbored the SHV-type ESBL gene. Finally, one out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 despite the lack of phenotypically proven resistance to imipenem. With the identification of novel ESBL gene variant and the demonstrated expansion of E. coli sequence types in equine patients, this study underscores the need for more investigation of equines as reservoirs for ESBL-producing pathogens. ]. The wide application of beta-lactam antibiotics has been considered as a driving factor in the development and spread of extended-spectrum beta-lactamase (ESBL)-conferred resistance in Gram-negative bacterial pathogens such as Escherichia coli, Klebsiella spp., and Sa...

show abstract

Section: Discussionmentioning

confidence: 55%

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

View full text Add to dashboard Cite

show abstract

“…According to Partridge and Hall (7), the similarity between Tn2 and Tn3 has corroborated the misannotation of Tn2 as Tn3. In fact, a Tn2 or ΔTn2 transposon located upstream of rmtB has been recognized by only a few authors (8)(9)(10)(11)(12). Two factors have contributed to the misannotation of Tn2 as Tn3: the high similarity found between these two transposons and an annotation error in the databases.…”

mentioning

confidence: 99%

Frequent Tn 2 Misannotation in the Genetic Background of rmtB

Martins

Gales

2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 64%

Antimicrobial Resistance in Horses

2020

View full text Add to dashboard Cite

the immune response of cattle to botulism, and a master's degree (2010) in health administration. Dr. Steinman served as Head of the large animal department (2006-2013), veterinary teaching hospital, Koret School of Veterinary Medicine (KSVM-VTH). Since 2013, he has served as Director of KSVM-VTH. His research is focused on equine infectious diseases, mainly vector-borne diseases and antimicrobial resistance.

show abstract

Characterization of an rmtB-carrying IncI1 ST136 plasmid in avian Escherichia coli isolates from chickens

Cited by 4 publications

References 29 publications

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Frequent Tn 2 Misannotation in the Genetic Background of rmtB

Antimicrobial Resistance in Horses

Contact Info

Product

Resources

About