Dissipation, metabolism and sorption of pesticides used in fruit-packaging plants: Towards an optimized depuration of their pesticide-contaminated agro-industrial effluents

Karas, Panagiotis A.; Metsoviti, Maria N.; Zisis, Vasileios; Ehaliotis, Constantinos; Omirou, Michalis; Papadopoulou, Evangelia S.; Menkissoglou-Spiroudi, Urania; Manta, Stella; Komiotis, Dimitri; Karpouzas, Dimitrios G.

doi:10.1016/j.scitotenv.2015.05.086

Cited by 43 publications

(38 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1d). This is in agreement with the only other study which investigated the metabolism and dissipation of EQ in the soil environment (24). All other studies have looked into the metabolism of EQ in food and animal tissues.…”

Section: Discussionsupporting

confidence: 91%

“…TBZ and IMZ showed moderate to high persistence (Table 1) in accordance with previous reports which showed DT 50 of 41 to 135 days for IMZ (36,37), and 37 to 1100 days for TBZ (38,39). OPP, DPA, and EQ dissipated rapidly (DT 50 s Ͻ 2.2 days) in line with previous studies (4,24). The transformation of EQ was studied further, since it was found to be almost simultaneously oxidized in soil to QI and EQNL ( Fig.…”

Section: Discussionsupporting

confidence: 87%

“…Residues of TBZ, IMZ, OPP, EQ, and DPA were extracted from soil as described by Karas et al (24). Special care was given to minimize the transformation of EQ to its oxidation derivatives during extraction (in a dark cold room, at 4°C).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Land Spreading of Wastewaters from the Fruit-Packaging Industry and Potential Effects on Soil Microbes: Effects of the Antioxidant Ethoxyquin and Its Metabolites on Ammonia Oxidizers

Papadopoulou

Tsachidou

Sułowicz

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

c Thiabendazole (TBZ), imazalil (IMZ), ortho-phenylphenol (OPP), diphenylamine (DPA), and ethoxyquin (EQ) are used in fruitpackaging plants (FPP) with the stipulation that wastewaters produced by their application would be depurated on site. However, no such treatment systems are currently in place, leading FPP to dispose of their effluents in agricultural land. We investigated the dissipation of those pesticides and their impact on soil microbes known to have a key role on ecosystem functioning. OPP and DPA showed limited persistence (50% dissipation time [DT 50 ], 0.6 and 1.3 days) compared to TBZ and IMZ (DT 50 , 47.0 and 150.8 days). EQ was rapidly transformed to the short-lived quinone imine (QI) (major metabolite) and the more persistent 2,4-dimethyl-6-ethoxyquinoline (EQNL) (minor metabolite). EQ and OPP exerted significant inhibition of potential nitrification, with the effect of the former being more persistent. This was not reflected in the abundance (determined by quantitative PCR [qPCR]) of the amoA gene of ammonia-oxidizing bacteria (AOB) and archaea (AOA). Considering the above discrepancy and the metabolic pattern of EQ, we further investigated the hypothesis that its metabolites and not only EQ were toxic to ammonia oxidizers. Potential nitrification, amoA gene abundance, and amoA gene transcripts of AOB and AOA showed that QI was probably responsible for the inhibition of nitrification. Our findings have serious ecological and practical implications for soil productivity and N conservation in agriculturally impacted ecosystems and stress the need to include metabolites and RNAbased methods when the soil microbial toxicity of pesticides is assessed. During postharvest handling, fruits are subjected to treatments with fungicides like thiabendazole (TBZ), imazalil (IMZ), and ortho-phenyl phenol (OPP) (1, 2) and antioxidants like diphenylamine (DPA) and ethoxyquin (EQ) (3), resulting in the production of large volumes of pesticide-contaminated wastewaters. The direct environmental discharge of those effluents without prior depuration entails a serious risk for the integrity of receiving ecosystems, considering the high aquatic toxicity of the pesticides (4, 5). This concern has been addressed by the European Commission (EC), and authorization for those pesticides was given with the stipulation "that an efficient treatment of the produced wastewaters should be operative at a local scale" (6, 7).Various processes and systems have been tested for the treatment of wastewaters produced by fruit-packaging plants (FPP) including adsorption onto activated carbon (8), photocatalytic degradation (9, 10), and biological degradation (11, 12). However, their high cost, their elevated technological requirements, and the frequent production of oxidized metabolites which are of unknown toxicity compared to the parent compound have hampered their full-scale implementation. Thus, to date effluents from FPP are either discharged in municipal wastewater treatment systems or they are disposed via land-spreading on the s...

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Land Spreading of Wastewaters from the Fruit-Packaging Industry and Potential Effects on Soil Microbes: Effects of the Antioxidant Ethoxyquin and Its Metabolites on Ammonia Oxidizers

Papadopoulou

Tsachidou

Sułowicz

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…This has forced most fruitpackaging industries to discharge their DPA-contaminated wastewater into nearby abandoned fields and evaporation ponds or directly into the municipal sewage treatment system, increasing the likelihood for environment contamination and degradation of ecosystems quality. Although DPA is not persistent in the soil environment (Karas et al 2015), the continuous disposal of DPA-contaminated effluents in soil could lead to the accumulation of DPA which need to be removed or else the contaminated soil could further acts as a sink for the subsequent transfer of DPA residues in adjacent natural water bodies (Drzyzga 2003). Alternatively, those wastewaters are collected by certified companies which treat them ex situ as toxic wastes, a particularly costly procedure for the owners of fruit-packaging plants (Karanasios et al 2012).…”

Section: Responsible Editor: Robert Duranmentioning

confidence: 99%

Isolation of a diphenylamine-degrading bacterium and characterization of its metabolic capacities, bioremediation and bioaugmentation potential

Perruchon

Batianis

Zouborlis

et al. 2015

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

The antioxidant diphenylamine (DPA) is used in fruit-packaging plants for the control of the physiological disorder apple scald. Its use results in the production of DPA-contaminated wastewater which should be treated before finally discharged. Biological treatment systems using tailored-made microbial inocula with specific catabolic activities comprise an appealing and sustainable solution. This study aimed to isolate DPA-degrading bacteria, identify the metabolic pathway of DPA and evaluate their potential for future implementation in bioremediation and biodepuration applications. A Pseudomonas putida strain named DPA1 able to rapidly degrade and utilize DPA as the sole C and N source was enriched from a DPA-contaminated soil. The isolated strain degraded spillage-level concentrations of DPA in liquid culture (2000 mg L(-1)) and in contaminated soil (1000 mg kg(-1)) and metabolized DPA via the transient formation of aniline and catechol. Further evidence for the bioremediation and biodepuration potential of the P. putida strain DPA1 was provided by its capacity to degrade the post-harvest fungicide ortho-phenylphenol (OPP), concurrently used by the fruit-packaging plants, although at slower rates and DPA in a wide range of pH (4.5-9) and temperatures (15-37 °C). These findings revealed the high potential of the P. putida strain DPA1 for use in future soil bioremediation strategies and/or as start-up inocula in wastewater biodepuration systems.

show abstract

“…O‐phenylphenol (OPP) (p K a = 9.4; log Po/ w = 3.18; solubility in water = 700 mg/L) is a general biocide, with germicide, fungicide, bactericide, and nematicide activity, usually supplied as its sodium salt . Because of their large variety of applications and massive use (Supporting Information Material A) , TBZ and OPP can be found in wastewaters from diverse sources, such as urban sewage systems, agro‐food industry discharges, and agricultural gutters . From there, they can diffuse into the atmosphere and may arrive to the environmental water .…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a method to determine thiabendazole and o‐phenylphenol in wastewater using micellar liquid chromatography‐fluorescence detection

et al. 2016

View full text Add to dashboard Cite

Registro de acceso restringido Este recurso no está disponible en acceso abierto por política de la editorial. No obstante, se puede acceder al texto completo desde la Universitat Jaume I o si el usuario cuenta con suscripción. Registre d'accés restringit Aquest recurs no està disponible en accés obert per política de l'editorial. No obstant això, es pot accedir al text complet des de la Universitat Jaume I o si l'usuari compta amb subscripció. Restricted access item This item isn't open access because of publisher's policy. The full--text version is only available from Jaume I University or if the user has a running suscription to the publisher's contents.

show abstract

Dissipation, metabolism and sorption of pesticides used in fruit-packaging plants: Towards an optimized depuration of their pesticide-contaminated agro-industrial effluents

Cited by 43 publications

References 38 publications

Land Spreading of Wastewaters from the Fruit-Packaging Industry and Potential Effects on Soil Microbes: Effects of the Antioxidant Ethoxyquin and Its Metabolites on Ammonia Oxidizers

Land Spreading of Wastewaters from the Fruit-Packaging Industry and Potential Effects on Soil Microbes: Effects of the Antioxidant Ethoxyquin and Its Metabolites on Ammonia Oxidizers

Isolation of a diphenylamine-degrading bacterium and characterization of its metabolic capacities, bioremediation and bioaugmentation potential

Development and validation of a method to determine thiabendazole and o‐phenylphenol in wastewater using micellar liquid chromatography‐fluorescence detection

Contact Info

Product

Resources

About