Dissipation monitoring for assessing EGF-induced changes of cell adhesion

Chen, Jennifer Y.; Shahid, Ammar; Garcia, Marcela P.; Penn, Lynn S.; Xi, Jun

doi:10.1016/j.bios.2012.06.018

Cited by 44 publications

(55 citation statements)

References 63 publications

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“…From a molecular point of view, Chen investigated the effects of EGF on cancer cells [18], while Prakrankamanant et al implemented a biosensor for the sensing of Papillomavirus type 58 [19].…”

Section: Availability Of Cdnas and Clones;mentioning

confidence: 99%

NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to Cancer

Bragazzi¹

2014

J Carcinog & Mutagen

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In this manuscript, we report some proofs-of-principle and preliminary applications and results regarding the analytical quantification of protein expression of some genes biologically and clinically related to cancer. Experiments have been carried out coupling Nucleic Acid Programmable Protein Array (NAPPA) with a recently improved nanogravimetric apparatus which exploits the quartz crystal microbalance with frequency (QCM_F) and quartz crystal microbalance with dissipation monitoring (QCM_D) technologies. The selected proteins are BRIP1, JUN and ATF2. Clinically relevant implications for cancer are envisaged and discussed, as well as future perspectives and developments.

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Section: Availability Of Cdnas and Clones;mentioning

confidence: 99%

NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to Cancer

Bragazzi¹

2014

J Carcinog & Mutagen

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“…Only a few QCM research studies have used QCM for sensing both short- and long-term changes in cellular viscoelastic properties in more biologically relevant situations, and they tend to be limited. Elsom et al (Elsom et al, 2008) used QCM to examine epithelial cell uptake of microspheres, and Chen et al (Chen et al, 2012) employed QCM to study the effects of epidermal growth factor on cell mechanics. These are reviewed along with other studies in (Saitakis and Gizeli, 2011) and (Xi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Chemically grafted fibronectin for use in QCM-D cell studies

Kandel

Lee

Sobolewski

et al. 2014

Biosensors and Bioelectronics

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Traditionally, fibronectin has been used as a physisorbed surface coating (physFN) in cell culture experiments due to its critical role in cell adhesion. However, because the resulting layer is thick, unstable, and of unpredictable uniformity, this method of fibronectin deposition is unsuitable for some types of research, including quartz crystal microbalance (QCM) experiments involving cells. Here, we present a new method for chemical immobilization of fibronectin onto silicon oxide surfaces, including QCM crystals pre-coated with silicon oxide. We characterize these chemically coated fibronectin surfaces (chemFN) as well as physFN ones using surface ellipsometry (SE), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. A cell culture model demonstrates that cells on chemFN and physFN surfaces exhibit similar viability, structure, adhesion and metabolism. Finally, we perform QCM experiments using cells on both surfaces which demonstrate the superior suitability of chemFN coatings for QCM research, and provide real-time QCM-D data from cells subjected to an actin depolymerizing agent. Overall, our method of chemical immobilization of fibronectin yields great potential for furthering cellular experiments in which thin, stable and uniform coatings are desirable. As QCM research with cells has been rather limited in success thus far, we anticipate that this new technique will particularly benefit this experimental system by availing it to the much broader field of cell mechanics.

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“…Both surface plasmon-based infrared spectroscopy (Yashunsky et al, 2012) and resonant waveguide grating (RWG) biosensor (Fang et al, 2006; Horvath et al, 2008) enable cell sensing with multiple sampling depths, so it is possible to resolve the multi-staged process of the adhesion and monolayer formation of Madin-Darby canine kidney (MDCK) cells (Yashunsky et al, 2012), and to detect the inhomogeneity in refractive index within the distinct layers of a fibroblast cell perpendicular to the biosensor surface during cell adhesion (Horvath et al, 2008). Furthermore, these biosensors are also useful to study receptor signaling that leads to alteration in adhesion pattern of cultured cells (Chen et al, 2012; Fang et al, 2005; Fang, 2010a; Garcia et al, 2013), and to decode the impact of ECM coatings on receptor signaling and drug pharmacology (Tran et al, 2012). However, few studies have been attempted to study the impact of the external stimuli-induced intracellular signaling, in particular, G protein-coupled receptor (GPCR) signaling, on the cell adhesion process per se .…”

Section: Introductionmentioning

confidence: 99%

Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion

Zaytseva

Lynn

et al. 2013

Sensors and Actuators B: Chemical

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Cell adhesion to extracellular matrix (ECM) is fundamental to many distinct aspects of cell biology, and has been an active topic for label-free biosensors. However, little attention has been paid to study the impact of receptor signaling on the cell adhesion process. We here report the development of resonant waveguide grating biosensor-enabled label-free and fluorescent approaches, and their use for investigating the adhesion of an engineered HEK-293 cell line stably expressing green fluorescent protein (GFP) tagged β2-adrenergic receptor (β2-AR) onto distinct surfaces under both ambient and physiological conditions. Results showed that cell adhesion is sensitive to both temperature and ECM coating, and distinct mechanisms govern the cell adhesion process under different conditions. The β2-AR agonists, but not its antagonists or partial agonists, were found to be capable of triggering signaling during the adhesion process, leading to an increase in the adhesion of the engineered cells onto fibronectin-coated biosensor surfaces. These results suggest that the dual approach presented is useful to investigate the mechanism of cell adhesion, and to identify drug molecules and receptor signaling that interfere with cell adhesion.

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Dissipation monitoring for assessing EGF-induced changes of cell adhesion

Cited by 44 publications

References 63 publications

NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to Cancer

NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to Cancer

Chemically grafted fibronectin for use in QCM-D cell studies

Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion

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