Passively mode-locked fiber lasers have been intensively applied in various research fields. However, the passive mode-locking typically operates in free-running regime, which easily produces messy multiple pulses due to the fruitful nonlinear effects involved in optical fibers. Actively controlling those disordered pulses in a passively mode-locked laser is of great interest but rarely studied. In this work, we experimentally investigate a flexible pulse-spacing manipulation in the passively mode-locked multipulse fiber laser by both intracavity and extracavity methods. A tuning range of pulse spacing up to 1.5 ns is achieved. More importantly, continuous pulse-spacing modulation is successfully demonstrated through external optical injection. It is anticipated that the results can contribute to the understanding of laser nonlinear dynamics and pursuing the optimal performance of passively mode-locked fiber lasers for practical applications. "Frequency metrology with a turnkey all-fiber system," Opt. Lett. 29(21), 2467-2469 (2004). 6. R. R. Gattass and E. Mazur, "Femtosecond laser micromachining in transparent materials," Nat. Photonics 2(4), 219-225 (2008). 7. F. M. Mitschke and L. F. Mollenauer, "Discovery of the soliton self-frequency shift," Opt. Lett. 11(10), 659-661 (1986). 8. M. Wanner, E. L. Tanzi, and T. S. Alster, "Fractional photothermolysis: treatment of facial and nonfacial cutaneous photodamage with a 1,550-nm erbium-doped fiber laser," Dermatol. Surg. 33(1), 23-28 (2007). 9. C. Xu and F. W. Wise, "Recent advances in fibre lasers for nonlinear microscopy," Nat. Photonics 7(11), 875-882 (2013). 10. C. W. Freudiger, W. Yang, G. R. Holtom, N. Peyghambarian, X. S. Xie, and K. Q. Kieu, "Stimulated Raman scattering microscopy with a robust fibre laser source," Nat. Photonics 8(2), 153-159 (2014). 11. B. E. Bouma, L. E. Nelson, G. J. Tearney, D. J. Jones, M. E. Brezinski, and J. G. Fujimoto, "Optical coherence tomographic imaging of human tissue at 1.55 μm and 1.81 μm using Er-and Tm-doped fiber sources," J.