Dissociated cortical networks show spontaneously correlated activity patterns during in vitro development

Chiappalone, Michela; Bove, Marco; Vato, Alessandro; Tedesco, Mariateresa; Martinoia, Sergio

doi:10.1016/j.brainres.2006.03.049

Cited by 363 publications

(379 citation statements)

References 64 publications

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“…Each channel was sampled at a frequency of 25 kHz and raw data were high-pass filtered at 200 Hz and low-pass filtered at 2 kHz. Spikes were sorted from the noise (10 V) using the threshold tool of the MCRack software calculated as a multiple of the standard deviation (7*SD) of the biological noise for each recording channel (Chiappalone et al, 2006). Sorted spikes were processed by using a personal developed software tool in Matlab environment calculating the array-wide synchronized electrodes as the sum of the electrodes that fired together into 100 ms bins over each 5 min recordings.…”

Section: Methodsmentioning

confidence: 99%

Key Electrophysiological, Molecular, and Metabolic Signatures of Sleep and Wakefulness Revealed in Primary Cortical Cultures

et al. 2012

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Although sleep is defined as a behavioral state, at the cortical level sleep has local and use-dependent features suggesting that it is a property of neuronal assemblies requiring sleep in function of the activation experienced during prior wakefulness. Here we show that mature cortical cultured neurons display a default state characterized by synchronized burst-pause firing activity reminiscent of sleep. This default sleep-like state can be changed to transient tonic firing reminiscent of wakefulness when cultures are stimulated with a mixture of waking neurotransmitters and spontaneously returns to sleep-like state. In addition to electrophysiological similarities, the transcriptome of stimulated cultures strikingly resembles the cortical transcriptome of sleep-deprived mice, and plastic changes as reflected by AMPA receptors phosphorylation are also similar. We used our in vitro model and sleep-deprived animals to map the metabolic pathways activated by waking. Only a few metabolic pathways were identified, including glycolysis, aminoacid, and lipids. Unexpectedly large increases in lysolipids were found both in vivo after sleep deprivation and in vitro after stimulation, strongly suggesting that sleep might play a major role in reestablishing the neuronal membrane homeostasis. With our in vitro model, the cellular and molecular consequences of sleep and wakefulness can now be investigated in a dish.

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Section: Methodsmentioning

confidence: 99%

Key Electrophysiological, Molecular, and Metabolic Signatures of Sleep and Wakefulness Revealed in Primary Cortical Cultures

et al. 2012

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“…By 14 DIV, cortical cultures reach a peak in synaptic density and neuronal cell connectivity (31), which reflects maturation of the neuronal network, paralleled by maturation of electrophysiological properties (32,33). We used the Y188 rabbit monoclonal antibody, which is highly specific for APP (34), and antiNav1.6 antibodies to stain 14 DIV cortical neuron cultures from WT mice and APP KO mice.…”

Section: App Is Colocalized With Nav16 In Mouse Cortical Neurons-mentioning

confidence: 99%

Amyloid Precursor Protein Enhances Nav1.6 Sodium Channel Cell Surface Expression

Liu

Tan

Xiao

et al. 2015

Journal of Biological Chemistry

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Background: Loss-of-function amyloid precursor protein (APP) and Nav1.6 transgenic mice share similar phenotypes. Results: APP interacts with Nav1.6 and enhances its cell surface expression through a G o -coupled JNK pathway. Conclusion: APP regulates Nav1.6 function, likely through a G o -coupled JNK pathway. Significance: This finding advances the current understanding of APP functions and has implications for novel therapies for neurodegenerative disorders.

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“…However, these previous studies focused mainly on early synaptic formation during early network construction. Network activity is known to show a mature pattern of synchronized bursting after random spontaneous activity in long-term culture (Kamioka et al, 1996;van Pelt et al, 2004;Chiappalone et al, 2006;Wagenaar et al, 2006). During this long-term development, however, how sufficient synaptic connections are established and how the density becomes saturated remain unclear.…”

mentioning

confidence: 99%

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

2013

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The aim of this study was to clarify the saturation processes of excitatory and inhibitory synapse densities during the long-term development of cultured neuronal networks. For this purpose, we performed a long-term culture of rat cortical cells for 35 days in vitro (DIV). During this culture period, we labeled glutamatergic and GABAergic synapses separately using antibodies against vesicular glutamate transporter 1 (VGluT1) and vesicular transporter of -aminobutyric acid (VGAT). The densities and distributions of both types of synaptic terminals were measured simultaneously. Observations and subsequent measurements of immunofluorescence demonstrated that the densities of both types of antibody-labeled terminals increased gradually from 7 to 21-28 DIV. The densities did not show a further increase at 35 DIV and tended to become saturated.Triple staining with VGluT1, VGAT, and microtubule-associated protein 2 (MAP2) enabled analysis of the distribution of both types of synapses, and revealed that the densities of the two types of synaptic terminals on somata were not significantly different, but that glutamatergic synapses predominated on the dendrites during long-term culture.However, some neurons did not fall within this distribution, suggesting differences in synapse distribution on target neurons. The electrical activity also showed an initial increase and subsequent saturation of the firing rate and synchronized burst rate during long-term culture, and the number of days of culture to saturation from the initial increase followed the same pattern under this culture condition.

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Dissociated cortical networks show spontaneously correlated activity patterns during in vitro development

Cited by 363 publications

References 64 publications

Key Electrophysiological, Molecular, and Metabolic Signatures of Sleep and Wakefulness Revealed in Primary Cortical Cultures

Key Electrophysiological, Molecular, and Metabolic Signatures of Sleep and Wakefulness Revealed in Primary Cortical Cultures

Amyloid Precursor Protein Enhances Nav1.6 Sodium Channel Cell Surface Expression

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

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