Dissociation between the interleukin 1-inducing capacity and Limulus reactivity of lipopolysaccharides from gram-negative bacteria

Laude-Sharp, Maryline; Haeffner‐Cavaillon, Nicole; Caroff, Martine; Lantreibecq, F.; Pusineri, Christian; Kazatchkine, Michel D.

doi:10.1016/1043-4666(90)90025-o

Cited by 55 publications

(28 citation statements)

References 22 publications

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“…This may mean that monocytes were activated to a greater extent during hemodialysis with RC membranes than during push/pull HDF with PS membranes, when the LPS level in dialysate was kept undetectable by a Limulus assay. To our knowledge, however, for the detection of LAL-negative pyrogens such as low-molecular-weight fragment of LPS a bioassay is needed to determine monocyte activation such as the production and release of cytokines from monocytes during treatment [25, 31]. We consider that this study was also a bioassay, since changes in Mac-1 and CD14 expression on monocytes and serum CD14 level may be considered as markers of monocyte activation [1, 2, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18].…”

Section: Discussionmentioning

confidence: 99%

Changes in Mac-1 and CD14 Expression on Monocytes and Serum Soluble CD14 Level during Push/Pull Hemodiafiltration

Kono¹,

Nakai²,

Misawa³

et al. 2002

Nephron

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Background/Aim: Employment of treated dialysate as replacement fluid raises concerns about exposure of patients to pyrogenic substances. This study was undertaken to evaluate the safety of treated dialysate as the replacement fluid for push/pull hemodiafiltration. Methods: In the present study, changes in the expressions of Mac-1 and CD14 on monocytes, which are upregulated by monocyte activation, were analyzed by flow cytometry, and the serum level of sCD14 which elevates by monocyte activation was measured by enzyme-linked immunosorbent assay (ELISA) during treatment in 7 patients on hemodialysis with regenerated cellulose (RC) membrane, polysulfone (PS) membranes and by push/pull hemodiafiltration (HDF) with PS membranes in a cross-over fashion. Results: During hemodialysis with RC, hemodialysis with PS or push/pull hemodiafiltration with PS, both Mac-1 and CD14 expressions on monocytes significantly increased by passing through the artificial kidneys, and, accordingly, the respective values downstream of the artificial kidneys were significantly higher than the predialysis values, even when the lipopolysaccharide level in dialysate was not detectable by Limulus assay. There was no significant variation in serum sCD14 levels during any of the hemodialysis with RC, hemodialysis with PS or push/pull hemodiafiltration. However, during hemodialysis with PS or push/pull hemodiafiltration with PS, changes in Mac-1 and CD14 expression on monocytes were significantly smaller than those during hemodialysis with RC. Conclusion: Monocytes are activated to a greater extent during hemodialysis with RC membranes than during push/pull HDF with PS membranes. We consider that push/pull HDF may be safer than hemodialysis with RC membrane and that it is as safe as hemodialysis with the PS membrane in terms of monocyte activation, when pyrogen-free dialysate is employed.

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Section: Discussionmentioning

confidence: 99%

Changes in Mac-1 and CD14 Expression on Monocytes and Serum Soluble CD14 Level during Push/Pull Hemodiafiltration

Kono¹,

Nakai²,

Misawa³

et al. 2002

Nephron

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“…Hansen et al (1999) found that LPS from Escherichia coli and Salmonella enteritis was about four times more potent than that from Klebsiella pneumoniae and Pseudomonas aeruginosa. Laude-Sharp et al (1990) found that E. coli LPS is about 100 times more potent than Shigella flexneri LPS and Pseudomonas testosteroni LPS is about 60% more active than E. coli LPS. Even within species there can be wide variations in LAL potency with a 625-fold range in some E. coli serotypes (Koyama et al 2000).…”

Section: Impeller Humidifiermentioning

confidence: 99%

“…This could be attributable to the species of bacteria generating the endotoxin, the amount of endotoxin actually respired, the size of particles with which the endotoxin is associated, and/or individual response variability (e.g. et al 1990;Hansen et al 1999;Koyama et al 2000). Table 1 summarizes some controlled laboratory studies involving human volunteers.…”

Section: Laboratory Endotoxin Inhalation Studies In Humansmentioning

confidence: 99%

Estimation of endotoxin inhalation from shower and humidifier exposure reveals potential risk to human health

Anderson

Dixon

Mayfield

2007

Journal of Water and Health

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This paper investigates potential exposure to endotoxin in drinking water through the inhalation of aerosols generated by showers and humidifiers. Adverse health effects attributable to the inhalation of airborne endotoxin in various occupational settings are summarized, as are controlled laboratory inhalation studies. Data from investigations estimating aerosolization of particulate matter by showers and humidifiers provide a basis for similar analyses with endotoxin, which like minerals in water, is nonvolatile. A theoretical assessment of the inhalation of aerosolized endotoxin showed that while the likelihood of an acute response while showering is minimal, the same is not true for humidifiers. Ultrasonic and impeller (cool mist) humidifiers efficiently produce large numbers of respirable particles. It is predicted that airway inflammation can occur if humidifier reservoirs are filled with tap water, sometimes even at typical drinkingwater distribution-system endotoxin concentrations. Higher endotoxin levels occasionally found in drinking water (.1,000 EU/ml) are very likely to induce symptoms such as chills and fever if used as humidifier feed water. While it is unlikely that treated drinking water would contain extremely high endotoxin levels occasionally observed in cyanobacterial blooms (.35,000 EU/ml), the potential for serious acute health consequences exist if used in humidifiers.

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“…The tumor necrosis factor alpha (TNF-α) production by human mononuclear cells was suppressed incompletely by polymyxin B, a nonspecific inhibitor of lipid A and LPS. Pereira et al [7]reported that bacterial cytokine-inducing substances from Pseudomonas aeruginosa were also not suppressed by polymyxin B. Laude-Sharp et al [8]presented experimental data showing that interleukin (IL) 1 inducing substances from gram-negative bacteria did not respond to Limulus amebocyte assay, a method to detect endotoxin and β-glucan, a component of fungi.…”

Section: Introductionmentioning

confidence: 99%

The Outer Membrane Protein I of <i>Pseudomonas aeruginosa </i>PAO1, a Possible Pollutant of Dialysate in Hemodialysis, Induces Cytokines in Mouse Bone Marrow Cells

Ino

Nagase²,

Nagasawa³

et al. 1999

Nephron

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The finding of outer membrane protein I (OprI) of Pseudomonas aeruginosa in hemodialyzers used by patients with end-stage renal failure led us to study the possible role of OprI as cytokine inducer. However, there are few reports on the biological activity of OprI, because it is difficult to obtain highly purified OprI. In this study, we attempt to establish a procedure for the efficient purification of OprI, which does not include lipopolysaccharide, from the bacterial culture broth, not hemodialyzers, to demonstrate that OprI is a potent cytokine inducer. From bacterial culture broth (1 liter), P. aeruginosa PAO1, which was confirmed previously by the sequence coding, was separated by centrifugation, high-performance liquid chromatography, and disk electrophoresis. Mouse bone marrow cells were stimulated by purified OprI, and the supernatants of the culture were analyzed by several enzyme-linked immunosorbent assay kits. The tumor necrosis factor alpha production stimulated by purified OprI was confirmed and degraded within 24 h. Furthermore, interleukin (IL) 1α, IL-1β, IL-6, and granulocyte-macrophage colony-stimulating factor were also induced by OprI despite the absence of lipopolysaccharide. We conclude that OprI has the potential to induce tumor necrosis factor alpha production in mouse bone marrow cells and that tumor necrosis factor alpha contributes to the induction of inflammatory cytokines, namely IL-1α, IL-1β, IL-6, and granulocyte/macrophage colony-stimulating factor, while lipopolysaccharide has little effect on these cells. These results suggest the presence of a pathway of inflammatory signal transduction triggered by OprI. In addition, OprI is possibly one of the harmful dialysate pollutants in hemodialysis patients besides the well-known lipopolysaccharide.

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Dissociation between the interleukin 1-inducing capacity and Limulus reactivity of lipopolysaccharides from gram-negative bacteria

Cited by 55 publications

References 22 publications

Changes in Mac-1 and CD14 Expression on Monocytes and Serum Soluble CD14 Level during Push/Pull Hemodiafiltration

Changes in Mac-1 and CD14 Expression on Monocytes and Serum Soluble CD14 Level during Push/Pull Hemodiafiltration

Estimation of endotoxin inhalation from shower and humidifier exposure reveals potential risk to human health

The Outer Membrane Protein I of <i>Pseudomonas aeruginosa </i>PAO1, a Possible Pollutant of Dialysate in Hemodialysis, Induces Cytokines in Mouse Bone Marrow Cells

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