Dissociation Between the Time Course of Ethanol and Extracellular Dopamine Concentrations in the Nucleus Accumbens After a Single Intraperitoneal Injection

Yim, Hyeon Joo; Robinson, Donita L.; White, Martha L.; Jaworski, Jason N.; Randall, Patrick K.; Lancaster, Francine E.; Gonzales, Rueben A.

doi:10.1097/00000374-200006000-00006

Cited by 11 publications

(22 citation statements)

References 26 publications

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“…Consistent with these results, EtOH (2 g/kg) increased DA more in SI relative to GH animals in the current study. The current 2 g/kg data in GH animals are consistent with previous work showing an equal increase in DA following 1 and 2 g/kg EtOH in rats housed under standard conditions (Yim et al, 2000). The augmented DA elevations in the SI animals at a 2 g/kg dose of EtOH may be involved in the increased consumption and seeking of EtOH observed in SI rats (McCool and Chappell, 2009; Chappell et al, 2013).…”

Section: Discussionsupporting

confidence: 91%

“…Acute EtOH elevates DA in the NAc when administered systemically (Yim et al, 2000), primarily via the activation of DA neurons in the ventral tegmental area (VTA) (Brodie et al, 1999; Ding et al, 2009). In contrast to a large body of literature investigating the effects of acute EtOH on DA, there is only one report on the effects of acute EtOH on NE in the NAc (Marinelli et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Social Isolation Rearing Increases Nucleus Accumbens Dopamine and Norepinephrine Responses to Acute Ethanol in Adulthood

Karkhanis

Locke

McCool

et al. 2014

Alcoholism Clin & Exp Res

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BACKGROUND Early-life stress is associated with increased vulnerability to alcohol addiction. However, the neural substrates linking chronic childhood/adolescent stress and increased risk of alcohol addiction are not well understood. In the nucleus accumbens (NAc), dopamine (DA) and norepinephrine (NE) signaling can be profoundly influenced by stress, anxiety, and drugs of abuse, including ethanol. Here, we employed a rodent model of early-life stress that results in enduring increases in behavioral risk factors of alcoholism to gain a better understanding of how chronic adolescent stress may impact the ethanol sensitivity of DA and NE release in the NAc. METHODS Male Long Evans rats were either group housed (GH; 4 rats/cage) or socially isolated (SI; 1 rat/cage) for six weeks beginning on postnatal day 28. SI and GH rats were tested in adulthood for anxiety-like behaviors (elevated plus-maze), and the effects of ethanol (1 and 2 g/kg; i.p.) on NAc DA and NE were assessed by microdialysis. RESULTS SI animals showed increased anxiety-like behavior compared to GH animals. Although SI had no effect on baseline levels of DA or NE, baseline DA levels were positively correlated with anxiety measures. In addition, while no significant differences were observed with 1 g/kg ethanol, the 2 g/kg dose induced significantly greater DA release in SI animals. Moreover, EtOH (2 g/kg) only elevated NAc NE levels in SI rats. CONCLUSIONS These results suggest that chronic early-life stress sensitizes accumbal DA and NE release in response to an acute ethanol challenge. A greater EtOH sensitivity of DA and NE release dynamics in the NAc may contribute to increases in behavioral risk factors of alcoholism, like greater ethanol self-administration, that are observed in SI rats.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Social Isolation Rearing Increases Nucleus Accumbens Dopamine and Norepinephrine Responses to Acute Ethanol in Adulthood

Karkhanis

Locke

McCool

et al. 2014

Alcoholism Clin & Exp Res

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“…Accordingly, the ethanol-induced release of β-endorphin and Met-enk from the NAcc has been demonstrated (Marinelli et al 2003(Marinelli et al , 2005Méndez et al in preparation). The ethanol-induced changes in pro-enk mRNA expression observed in this study correlate with high ethanol concentrations in plasma (Li et al 1998;Méndez et al 2001) and NAcc (Yim et al 2000), as well as with the acute tolerance to ethanol-induced DA release in the NAcc developed after a single acute dose of ethanol (Yim et al 2000). Therefore, our results are consistent with the reported positive reinforcing actions of ethanol in the mesocorticolimbic system (Di Chiara et al 1996;Koob et al 1998;Yim et al 2000) and support a role for enkephalins in these mechanisms.…”

Section: Discussionsupporting

confidence: 89%

Ethanol exposure differentially alters pro-enkephalin mRNA expression in regions of the mesocorticolimbic system

Méndez

Morales-Mulia

2006

Psychopharmacology

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“…This is an interesting finding since ethanol associated cues and operant self-administration of ethanol both increase dopamine release in the “shore” following operant training (Howard et al, 2009), but in the core or shell only ethanol associated cues increase dopamine release following operant training. It is important to note, however, that acute systemic ethanol administration increases dopamine release in the NAc core and shell (Imperato and Di Chiara, 1986; Yim et al, 2000) where ethanol did not alter 3α,5α-THP levels. Administration of 3α,5α-THP produces biphasic effects on ethanol consumption (Janak et al, 1998; Ford et al, 2005; Ford et al, 2007; Finn et al, 2010) and dopamine release in the NAc (Motzo et al, 1996; Rouge-Pont et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Ethanol Administration Produces Divergent Changes in GABAergic Neuroactive Steroid Immunohistochemistry in the Rat Brain

Cook

Dumitru

O'Buckley

et al. 2013

Alcohol Clin Exp Res

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Background The 5α-reduced pregnane neuroactive steroid (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP or allopregnanolone) is a potent positive modulator of GABAA receptors capable of modulating neuronal activity. In rats, systemic ethanol administration increases cerebral cortical and hippocampal levels of 3α,5α-THP, but the effects of ethanol on 3α,5α-THP levels in other brain regions are unknown. There is a large body of evidence suggesting that 3α,5α-THP enhances ethanol sensitivity, contributes to some behavioral effects of ethanol, and modulates ethanol reinforcement and motivation to drink. In the present study, we used immunohistochemistry (IHC) to determine ethanol-induced changes in cellular 3α,5α-THP expression in brain regions associated with ethanol actions and responses. Methods Male Wistar rats were administered ethanol (2g/kg) or saline intraperitoneally and after 60 minutes transcardially perfused. IHC was performed on free floating sections (3–4 sections/animal/brain region) using an affinity purified anti-3α,5α-THP primary antibody and immunoreactivity was visualized with 3,3′-diaminobenzidine. Results Ethanol significantly increased 3α,5α-THP immunoreactivity by 24±6% in the medial prefrontal cortex, 32±12% in the hippocampal CA1 pyramidal cell layer, 52±5% in the polymorph cell layer of the dentate gyrus, 44±15% in the bed nucleus of the stria terminalis, and by 36±6% in the paraventricular nucleus of the hypothalamus. In contrast, ethanol administration significantly reduced 3α,5α-THP immunoreactivity by 25±5% in the nucleus accumbens “shore” and 21±3% in the central nucleus of the amygdala. No changes were observed in the ventral tegmental area, dorsomedial striatum, granule cell layer of the dentate gyrus, or the lateral and basolateral amygdala. Conclusions The results suggest acute ethanol (2g/kg) produces divergent, brain region specific, effects on cellular 3α,5α-THP levels. Regional differences in the effects of ethanol suggest there may be regional brain synthesis of 3α,5α-THP independent of the adrenal glands and novel mechanisms that reduce cellular 3α,5α-THP. Regional differences in ethanol-induced changes in 3α,5α-THP levels likely contribute to ethanol effects on neuronal function in brain.

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Dissociation Between the Time Course of Ethanol and Extracellular Dopamine Concentrations in the Nucleus Accumbens After a Single Intraperitoneal Injection

Cited by 11 publications

References 26 publications

Social Isolation Rearing Increases Nucleus Accumbens Dopamine and Norepinephrine Responses to Acute Ethanol in Adulthood

Social Isolation Rearing Increases Nucleus Accumbens Dopamine and Norepinephrine Responses to Acute Ethanol in Adulthood

Ethanol exposure differentially alters pro-enkephalin mRNA expression in regions of the mesocorticolimbic system

Ethanol Administration Produces Divergent Changes in GABAergic Neuroactive Steroid Immunohistochemistry in the Rat Brain

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