Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Hühn, Jonas; Fedeli, Chiara; Zhang, Qian; Masood, Atif; Pino, Pablo del; Khashab, Niveen M.; Papini, Emanuele; Parak, Wolfgang J.

doi:10.1016/j.biocel.2015.12.015

Cited by 50 publications

(36 citation statements)

References 83 publications

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“…As expected, the more proteins are attached per NP, the more the hydrodynamic radii increases, reaching saturation when the maximum possible amount of proteins is attached per NP. Similar measurements of NP–protein size vs. number of attached proteins could be recorded with gel electrophoresis 15 , but not with standard DLS, where the experimental error was higher than the actual changes in size.…”

Section: Resultssupporting

confidence: 55%

In situ detection of the protein corona in complex environments

et al. 2017

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Colloidal nanoparticles (NPs) are a versatile potential platform for in vivo nanomedicine. Inside blood circulation, NPs may undergo drastic changes, such as by formation of a protein corona. The in vivo corona cannot be completely emulated by the corona formed in blood. Thus, in situ detection in complex media, and ultimately in vivo, is required. Here we present a methodology for determining protein corona formation in complex media. NPs are labeled with 19F and their diffusion coefficient measured using 19F diffusion-ordered nuclear magnetic resonance (NMR) spectroscopy. 19F diffusion NMR measurements of hydrodynamic radii allow for in situ characterization of NPs in complex environments by quantification of protein adsorption to the surface of NPs, as determined by increase in hydrodynamic radius. The methodology is not optics based, and thus can be used in turbid environments, as in the presence of cells.

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Section: Resultssupporting

confidence: 55%

In situ detection of the protein corona in complex environments

et al. 2017

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“…The corona is therefore presumed to consist of a “hard” corona (surface‐bound) and the “soft” corona (in exchange with surrounding proteins) . Note that in our interpretation the difference between the “soft” and “hard” corona simply relates to different protein–NP dissociation constants . Our results clearly indicate a shift in the protein constituents of the corona over the incubation time periods investigated (15 min to 24 h): The most abundant proteins identified after 15 min devolved to a more diversified number of protein species after 24 h.…”

Section: Resultsmentioning

confidence: 58%

“…From a bottom‐up approach one can make model systems, in which only one type of protein is added to one type of NP. In this case, protein adsorption can be investigated quantitatively, allowing for the extraction of kinetics and equilibrium values such as dissociation constants, number of adsorbed proteins per NP, etc . Going from one type to two types of adsorbed proteins would be the next step toward a more realistic scenario.…”

Section: Introductionmentioning

confidence: 99%

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

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Pfeiffer

et al. 2017

Adv Funct Materials

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To study the influence of colloidal stability on protein corona formation, gold nanoparticles are synthesized with five distinct surface modifications: coating with citric acid, bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt, thiol-terminated methoxy-polyethylene glycol, dodecylaminegrafted poly(isobutylene-alt-maleic anhydride), and dodecylamine-grafted poly(isobutylene-alt-maleic anhydride) conjugated with polyethylene glycol. The nanoparticles are incubated with serum or bronchoalveolar lavage fluid from C57BL/6 mice (15 min or 24 h) to assess the effect of differential nanoparticle surface presentation on protein corona formation in the air-blood barrier exposure pathway. Proteomic quantification and nanoparticle size measurements are used to assess protein corona formation. We show that surface modification has a clear effect on the size and the composition of the protein corona that is related to the colloidal stability of the studied nanoparticles. Additionally, differences in the composition and size of the protein corona are shown between biological media and duration of exposure, indicating evolution of the corona through this exposure pathway. Consequently, a major determinant of protein corona formation is the colloidal stability of nanoparticles in biological media and chemical or environmental modification of the nanoparticles alters the surface presentation of the functional epitope in vivo. Therefore, the colloidal stability of nanoparticles has a decisive influence on nano-bio interactions.

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“…It is worth mentioning that after the first introduction of particulate drug carriers into biological fluids, organic compounds from these fluids tend to bind carriers' surface forming so-called protein corona [16]. This protein coating may undergo continuous adsorption and desorption, depending on the affinity of the surrounding proteins to the carrier's surface [17]. Protein corona can significantly change physicochemical properties of the carriers [18].…”

Section: Introductionmentioning

confidence: 99%

Optically responsive delivery platforms: from the design considerations to biomedical applications

et al. 2020

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Drug carriers with intelligent functions are powerful therapeutic and diagnostic platforms in curing various diseases such as malignant neoplasms. These functions include the remote noninvasive activation of drug using physical impacts, e.g. light exposure. Combination of different therapeutic modalities (chemotherapy, photodynamic therapy, and so forth) with light-responsive carriers enables promising synergetic effect in tumour treatment. The main goal of this review article is to provide the state of the art on light-sensitive delivery systems with the identification of future directions and their implementation in tumour treatment. In particular, this article reviews the general information on the physical and chemical fundamental mechanisms of interaction between light and carrier systems (e.g. plasmonic and dielectric nanoparticles), the design of optically responsive drug carriers (plain and composite), and the mechanisms of light-driven controlled release of bioactive compounds in biological environment. The special focus is dedicated to the most recent advances in optically responsive bioinspired drug vehicles.

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Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Cited by 50 publications

References 83 publications

In situ detection of the protein corona in complex environments

In situ detection of the protein corona in complex environments

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

Optically responsive delivery platforms: from the design considerations to biomedical applications

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