Dissociation of Adult Mouse Liver by Sodium Tetraphenylboron, A Potassium Gomplexing Agent.

Rappaport, Catherine; Howze, Gwendolyn B.

doi:10.3181/00379727-121-30951

Cited by 72 publications

(6 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite their altered permeability properties, mechanically prepared cells still retain the capacity to recognize the original tissue, and form reaggregated cell colonies when injected intraperitoneally into a second animal (Laws & Stickland, 1961;Bhargava, 1970). Again cells isolated by the method of Rappaport & Howze (1966) with tetraphenylboron are reported to be metabolically inert (Friedman & Epstein, 1967;Murthy & Petering, 1969;Suzangar & Dickson, 1970), but have been used successfully as starting material for cell culture over periods of more than 6 months (Gerschenson & Casanello, 1968); these cultured cells synthesize glycogen and retain sensitivity to insulin (Gerschenson & Andersson, 1971).…”

Section: Studies With Mechanically Prepared Cellsmentioning

confidence: 99%

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Crisp

Pogson

1972

Biochemical Journal

130

View full text Add to dashboard Cite

Parenchymal cells contain both glucose 6-phosphatase and fructose 1,6-diphosphatase. The non-parenchymal fraction appears to contain fructose 1,6-diphosphatase, but is devoid of glucose 6-phosphatase. 8. No aldolase A was detectable in the whole liver. Aldolase B occurs in both parenchymal and non-parenchymal tissue. 9. Parenchymal cells prepared by mechanical disruption ofmouse liver with 20 % polyvinyl alcohol exhibit a similar enzyme profile to those prepared enzymically. 10. The methodology involved in the preparation of isolated liver cells is discussed. The importance of the measurement of several parameters as criteria for establishing the viability of parenchymal cells is stressed. 11. The metabolic implications of the results in the present study are discussed.

show abstract

Section: Studies With Mechanically Prepared Cellsmentioning

confidence: 99%

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Crisp

Pogson

1972

Biochemical Journal

130

View full text Add to dashboard Cite

show abstract

“…In general, it is recognized that the calcium ions (Ca++) play an important role in maintenance of cell contact in intact tissue [9], and similar function has been assigned to Mg++ [9] and K+ [21]. On the other hand, Seglen [23] stated that the cellular adhesion was specifically dependent on Ca++, and was not maintained if Ca++ was replaced by Mg++ in tissue culture medium.…”

mentioning

confidence: 99%

Effect of Calcium on the Cell Infectivity of Virulent Shigella flexneri 2a

Osada

Une

Ikeuchi

et al. 1974

Japanese Journal of Microbiology

View full text Add to dashboard Cite

Effect of calcium ions (Ca++) on the virulence of Shigellaflexneri 2a was examined with reference to its infectivity to cultured cells, the guinea pig eye, and the ligated small intestine of rabbits. The organism grown in a calcium-containing medium showed a significantly higher ability to penetrate HeLa cells than that of organism grown in a calcium-deficient medium. This ability was constantly maintained in the presence of Ca++, while readily lost in the absence of Ca++. Similarly, its pathogenicity for the guinea pig eye and the ligated small intestine of rabbits was more or less greater in cases with Ca++ than in cases without Ca++. Ca++ seemed to play an important role in bacterial adhesion to the cell membrane, by which the cellular engulfment was induced. The presence of Ca++ in the environment of bacterial growth was emphasized on development and maintenance of virulence of S. flexneri 2a.

show abstract

“…Various methods have been used to prepare the isolated liver cells: mechanical dispersion [25], chelation of intercellular Ca 2+ with citrate [9] or ethylenediamine tetraacetic acid [22], chelation of K ÷ with tetraphenyl boron [24], dissolution of intercellular matrix with proteolytic enzymes [8,23].…”

Section: Introductionmentioning

confidence: 99%

Comparison of cytologic and biochemical properties between liver cells isolated from adult rats by trypsin perfusion and those isolated by collagenase perfusion

et al. 1984

View full text Add to dashboard Cite

Primary cultures of liver cells isolated from adult rats by trypsin and collagenase perfusion techniques were carried out to compare cytologic and biochemical properties between the differently prepared cells. Trypsin-dispersed cells consisted of comparatively smaller cells, whereas collagenase-dispersed cells consisted of larger cells. The cell attachment efficiency on culture day 1 was about twice as high in the liver cells prepared with collagenase than those prepared with trypsin. Mature hepatocytes isolated by collagenase perfusion could be maintained in the primary culture for a longer period than those isolated by trypsin perfusion. Epithelial-like clear cells started to grow much earlier in the primary culture of the trypsin-dispersed liver cells than in that of the collagenase-dispersed liver cells. Earlier proliferation of epithelial-like clear cells could not be induced by in vitro trypsinization of the collagenase-dispersed liver cells. Both kinds of enzymatically prepared liver cells showed albumin production and exhibited glucose 6-phosphatase (D-glucose-6-phosphate phosphohydrolase, EC 3.1.3.9, G6Pase) and tyrosine aminotransferase (L-tyrosine: 2-oxoglutarate amino-transferase, EC 2.6.1.5, TAT) activities for 1 week in the primary culture. Albumin production was higher in the liver cells prepared with collagenase than those prepared with trypsin, whereas G6Pase activity was almost the same between them. TAT activity up to culture day 2 was about 3-fold higher in the liver cells prepared with collagenase than in those prepared with trypsin. Combined supplementation of dexamethasone (1 X 10(-5)M) and insulin (10 micrograms/ml) consistently improved the cell attachment efficiency and was very effective in the maintenance of mature hepatocytes in both types. Furthermore, these hormones enhanced the albumin production and TAT activity in both types.

show abstract

Dissociation of Adult Mouse Liver by Sodium Tetraphenylboron, A Potassium Gomplexing Agent.

Cited by 72 publications

References 1 publication

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Effect of Calcium on the Cell Infectivity of Virulent Shigella flexneri 2a

Comparison of cytologic and biochemical properties between liver cells isolated from adult rats by trypsin perfusion and those isolated by collagenase perfusion

Contact Info

Product

Resources

About