Dissociation of M- and N-group mucoproteins in subunits in detergen solutions

Morawiecki, A

doi:10.1016/0926-6526(64)90012-6

Cited by 33 publications

(13 citation statements)

References 5 publications

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“…Amino acid analyses of these preparations, reveal high proportions of threonin, serin and glutamic acid and confirm earlier findings of Uhlenbruck and Weber [45]. The carbohydrate content of Kathan's preparation is presented in Table VIII; the following molar distribution for both the M-and N-active compound may be derived on the basis of a molecular weight of 30,000 [28,40] : 23 moles sialic acid, 16 moles N-acetyl-galactosamine, 8 moles N-acetylglucosamine, and 24 moles galactose. This distribution implies that in both M-and N-substance the molar proportions of sialic acid: hexosamine: hexose equal approximately 1:1:1.…”

Section: Red Blood Cell Membrane Constituants With Blood Group Activisupporting

confidence: 71%

Blood Group Antigens in Relation to Chemical and Structural Properties of the Red Cell Membrane

Zahler¹

1968

Vox Sanguinis

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(1) The composition of red-cell-lipids and -proteins has been reviewed with special regard to recent data on the chemistry and physico-chemistry of membrane proteins. (2) A new concept for the architecture of the cell-membrane is proposed, which takes into account several possibilities for conformational arrangements of membrane proteins. (3) Red-cell-membrane lipids and -proteins have been discussed with regard to their possible functions as carriers of blood group activity. AB-specificity could be demonstrated not only in glycolipids but in the erythrocyte membrane proteins as well.

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Section: Red Blood Cell Membrane Constituants With Blood Group Activisupporting

confidence: 71%

Blood Group Antigens in Relation to Chemical and Structural Properties of the Red Cell Membrane

Zahler¹

1968

Vox Sanguinis

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“…The results of ultracentrifugal studies suggest that these substances can form large molecular aggregates and dissociate into smaller subunits in the presence of detergents. Such aggregation phenomena are not uncommon in the substances isolated from cell membranes, for example the MN-active antigens isolated from the red cells show the same properties [38]. The value of the sedimentation constant of the antigens in the presence of 0.5 O/, sodium dodecylsulphate roughly corresponds to a molecular weight of 50000.…”

Section: Discusslonmentioning

confidence: 96%

New Form of A‐, B‐, and H‐Blood‐Group‐Active Substances Extracted from Erythrocyte Membranes

Gardas

Kościelak

1973

European Journal of Biochemistry

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The isolation and purification of new group A-, B-and H-specific substances from human erythrocyte membranes is described. These substances contain galactose, fucose, glucosamine, sialic acid and amino acid residues. The A-specific substance also contains galactosamine. They contain a t most about Z0I0 fatty acids or sphingosine. The substances are serologically active; however, both required combination with phospholipids for development of maximum activity ine haemagglutination inhibition tests. Addition of phospholipids was not required in serological precipitation tests. These blood-group substances may be used to coat group-0 or Bombay-Oh red cells, thereby conferring on them A-, B-, or H-activity. They form high-molecular-weight aggregates in aqueous solution but disaggregate into smaller units in the presence of sodium dodecylsulphate.The glycolipid nature of A-and B-antigens in human erythrocytes was established in the last decade [1,2] However some recent evidence obtained in different laboratories [lo-141 indicated that glycoprotein substances specific for blood-group A, B and H occur in human erythrocytes as well as glycolipids. This paper presents an attempt to purify and characterise immunologically and chemically these recently discovered substances. A Preliminary account of this work was presented in an abstract form [15].Enzymes. a-l,2-~-E'ucosidase (EC 3.2.1.-) ; a-N-acetylgalactosaminidase (EC 3.2.1.-); B-galactosidase (EC 3.2.1.23). MATERIALS AND METHODS Preparation of Erythrocyte StromaErythrocyte stroma were prepared from the outdated blood by the toluene flotation technique of Kobcielak et al. [3]. The dried stroma were extracted with 83O/, ethanol and the extract cooled and centrifuged to remove the active glycolipids [16]. Half of the volume of the supernatant was then warmed to room temperature and used for reextraction of the stroma powder left after the first ethanol extraction. This procedurc was repeated once more, the dissolved glycolipids being removed each time by cooling and centrifuging the extract. The final yield of ethanol-extracted stroma ranged from 20 t o 25 g per liter of packed erythrocytes. Xerological AssaysHaemagglutination inhibition tests were performed by the method of Morgan and King [17]. Normal human anti-A sera were used for the determination of A-activity. For the determination of H-activity the sera were prepared by immunisation of rabbits with human-cyst H-substance coupled to the protein component of somatic-0 antigen of Xhigella

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“…This has been well documented for the glycoproteins with activity M and N pre pared from red cells [2,12,18] and, more generally, for the protein compo nents of erythrocytes stroma after the extraction of the lipid fraction [1,11,15,16].…”

Section: Discussionmentioning

confidence: 99%

Extraction and Partial Purification of Blood Group Substances A, B and H from Erythrocyte Stroma

Liotta¹,

Quintiliani²,

Quintiliani³

et al. 1972

Vox Sanguinis

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Materials with blood group activity A, B and H have been obtained from erythrocyte stroma by phenol extraction and subsequent purification by chromatography on Sephadex G-200 and ultracentrifugation at 180,000 g for 4 h. The final materials contain carbohydrates, protein and lipids, have a specific activity similar to that of the glycolipids extracted from erythrocyte stroma by other workers, and are found not to be homogeneous on ultracentrifugal analysis. Treatment with 0.5% SDS results in the reduction of the average particle size and in the appearance of new units which sediment homogeneously. However, in the presence of SDS the greatest part of the specific activity is lost, while extraction with butanol drastically reduces the At specific activity and to some extent the B activity has no influence on the A and probably none on the H activity.

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Dissociation of M- and N-group mucoproteins in subunits in detergen solutions

Cited by 33 publications

References 5 publications

Blood Group Antigens in Relation to Chemical and Structural Properties of the Red Cell Membrane

Blood Group Antigens in Relation to Chemical and Structural Properties of the Red Cell Membrane

New Form of A‐, B‐, and H‐Blood‐Group‐Active Substances Extracted from Erythrocyte Membranes

Extraction and Partial Purification of Blood Group Substances A, B and H from Erythrocyte Stroma

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