Dissociation of the behavioural and endocrine effects of lysine vasopressin by tryptic digestion

Wied, D. de; Greven, H. M.; Lande, Saul; Witter, A.

doi:10.1111/j.1476-5381.1972.tb09582.x

Cited by 170 publications

(36 citation statements)

References 14 publications

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“…Another possible mechanism by which peripherally administered oxytocin and vasopressin may affect the CNS is through the formation of active fragments that may cross the BBB. Fragments of both oxytocin and vasopressin have been shown to cross the BBB and affect memory processes (de Wied et al, 1972(de Wied et al, , 1987de Wied, 1976;de Wied and Versteeg, 1979;Walter et al, 1975;Burbach et al, 1983a,b;Tanabe et al, 1997;Rainbow et al, 1979). One promising strategy for peptide delivery to the brain is through chemical modification resulting in a peptide or fragment with favorable BBB permeability, which has important implications for the development of clinically relevant treatments.…”

Section: Discussionmentioning

confidence: 99%

Oxytocin Infusion Reduces Repetitive Behaviors in Adults with Autistic and Asperger's Disorders

Hollander

Novotny

Hanratty

et al. 2003

Neuropsychopharmacol

624

403

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Autism is a neurodevelopmental disorder characterized by dysfunction in three core behavioral domains: repetitive behaviors, social deficits, and language abnormalities. There is evidence that abnormalities exist in peptide systems, particularly the oxytocin system, in autism spectrum patients. Furthermore, oxytocin and the closely related peptide vasopressin are known to play a role in social and repetitive behaviors. This study examined the impact of oxytocin on repetitive behaviors in 15 adults with autism or Asperger's disorder via randomized double-blind oxytocin and placebo challenges. The primary outcome measure was an instrument rating six repetitive behaviors: need to know, repeating, ordering, need to tell/ask, self-injury, and touching. Patients with autism spectrum disorders showed a significant reduction in repetitive behaviors following oxytocin infusion in comparison to placebo infusion. Repetitive behavior in autism spectrum disorders may be related to abnormalities in the oxytocin system, and may be partially ameliorated by synthetic oxytocin infusion.

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Section: Discussionmentioning

confidence: 99%

Oxytocin Infusion Reduces Repetitive Behaviors in Adults with Autistic and Asperger's Disorders

Hollander

Novotny

Hanratty

et al. 2003

Neuropsychopharmacol

624

403

View full text Add to dashboard Cite

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“…Central mediation has been assumed on the basis that nanogram quantities of vasopressin and its behaviorally active fragments have similar effects when injected directly into the ventricular system (de Wied 1976;Bohus et al, 1978aBohus et al, , 1978b) and on recent evidence showing alterations in catecholamines from discrete brain nuclei following ventricular vasopressin injections (Tanaka, de Kloet, de Wied, & Versteeg 1977). Pressor and antidiuretic effects of the peptide were not thought to contribute significantly to its behavioral effects (Lande et al, 1971;de Wied et al, 1972). Recent evidence has shown that doses of AVP which prolong extinction responding also induce an early pressor response; conversely, injections of the antagonist peptide (dPTyr-(Me)AVP) block both the pressor and behavioral effects of vasopressin (Le Moal et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…Recent evidence has shown that doses of AVP which prolong extinction responding also induce an early pressor response; conversely, injections of the antagonist peptide (dPTyr-(Me)AVP) block both the pressor and behavioral effects of vasopressin (Le Moal et al, 1981). Although the receptors which mediate the behavioral effects of vasopressin appear to be similar to those involved in the mediation of pressor effects the role of the short-term pressor response per se in mediating the behavioral effects of the peptide is controversial but seems unlikely in view of findings that des-glycinamide vasopressin analogs lack pressor potency despite their behavioral activity (Lande et al, 1971;de Wied et al, 1972). Furthermore, post-training peripheral injections of antivasopressin serum which abolish the endogenous peripheral peptide, as shown by diuresis, did not alter inhibitory (passive) avoidance behavior whereas much smaller quantities of ventricularly injected antivasopressin serum altered behavior while not affecting peripheral vasopressin levels (van .…”

Section: Discussionmentioning

confidence: 99%

“…Further support for the memory hypothesis stems from observations that the des-glycinamide analogs of vasopressin, which lack virtually all antidiuretic and pressor activity, retain their behavioral potency (Lande, Witter, & de Wied 1971;de Wied, Greven, Lande, & Witter 1972) and protect against retrograde amnesias induced by puromycin (Walter~ Hoffmann, Flexner, & Flexner 1975;Flexner, Ftexner, Hoffmann, & Walter 1977;Flexner, Flexner, Walter, & Hoffmann 1978), anoxia (Rigter, van Reizen, & de Wied 1974), pentylenetetrazole (Bookin & Pfeifer 1977), and electroconvulsive shock (ECS) (Pfeifer and Bookin 1978). That these effects reflect a physiological role for the peptide is suggested by reports that nanogram quantities of arginine vasopressin delayed pole-jump extinction (de Wied 1976) and attenuated passive avoidance extinction (Bohus et al, 1978b;Bohus et al, 1978a) when injected into the lateral ventricles of the brain.…”

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confidence: 99%

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Post-training vasopressin injections may facilitate or delay shuttle-box avoidance extinction

Hagan

Bohus

Wied

1982

Behavioral and Neural Biology

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After training to avoid footshock in a two-way shuttle box rats were injected with lysine vasopressin (LVP) and returned to the shuttle box 24 hr later for 10 extinction trials. Experiment 1 shows that when injected 30 min after training subsequent extinction responding varied as an inverted "U"-shaped function of the LVP dose within the range tested (0.036 to 2.97 /xg/rat). Responding was increased with 0.11 /~g/rat whereas 2.97 tzg/rat reduced responding. Experiment 2 shows that these two doses also have opposite effects when injected 30 min after training at a higher shock level (0.45 mA/2 sec). Experiment 3 examined time-dependent effects for these oppositely acting doses and shows that whereas 0.11 txg/rat increased extinction responding when injected 0 or 60 min after training, 2.97 /zg/rat was ineffective when injected immediately and increased responding when injected 60 min after training. The data are discussed in relation to the hypothesis that vasopressin facilitates memory consolidation and with respect to anomalous time × dose interactions with various post-training drug treatments.

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“…The behavioural effects of these hormones are not due to their endocrine actions, for fragments of these peptides such as ACTH4-~0 and DG-LVP which are practically devoid of endocrine activity produce very similar effects (De Wied, 1967;De Wied et al, 1972). ACTH4-~0 has also been shown to affect visual attention in healthy humans Miller et al, 1974).…”

Section: Introductionmentioning

confidence: 99%

Effects of ACTH4–;10 on synaptic transmission in frog sympathetic ganglion

Wouters

Bercken

1979

European Journal of Pharmacology

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W. WOUTERS and J. VAN DEN BERCKEN, Effects of ACTH4-1o on synaptic transmission in frog sympathetic ganglion, European J. Pharmacol. 57 (1979) 353--363.The influence of ACTH4.10, a behaviourally active fragment of adrenocorticotropic hormone (ACTH)devoid of endocrine activity, on synaptic transmission in the paravertebral sympathetic ganglion of the frog was investigated. Postsynaptic potentials evoked by electrical stimulation of preganglionic nerves were recorded using a sucrose gap method. Fast excitatory postsynaptic potentials (EPSPs), which are mediated via nicotinic cholinergic synapses, were not affected by 10 -6 M ACTH4.10. Application of ACTH4.10 in a concentration as low as 10 -8 M for 60 rain caused a marked augmentation of the amplitude of slow inhibitory postsynaptic potentials (IPSPs) which are mediated via dopaminergic synapses. The increase in amplitude developed gradually after a latency of 60--90 rain and outlasted the application of the peptide. In addition, ACTH4.10 at 10 -6 M increased the hyperpolarising response of the ganglion to exogenous dopamine, as studied by a micro-application method. There was no significant effect of ACTH4-10 on the muscarinic cholinergic depolarising response of the ganglion towards exogenous acetylcholine. The behaviourally active vasopressin fragment DG-LVP (10 -6 M) had no effect on slow IPSPs. The results demonstrate that ACTH4-10 specifically affects slow synaptic inhibition in frog sympathetic ganglion, probably by acting upon the postsynaptic membrane. The possibility is discussed that ACTH4-10 affects one of the intermediate steps between dopaminergic receptor interaction and generation of the slow IPSP.Frog sympathetic ganglion Dopamine-induced hyperpolarisations Excitatory/inhibitory synaptic transmisson Sucrose gap method ACTH4.10 Neuropeptides

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Dissociation of the behavioural and endocrine effects of lysine vasopressin by tryptic digestion

Cited by 170 publications

References 14 publications

Oxytocin Infusion Reduces Repetitive Behaviors in Adults with Autistic and Asperger's Disorders

Oxytocin Infusion Reduces Repetitive Behaviors in Adults with Autistic and Asperger's Disorders

Post-training vasopressin injections may facilitate or delay shuttle-box avoidance extinction

Effects of ACTH4–;10 on synaptic transmission in frog sympathetic ganglion

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