1997
DOI: 10.1074/jbc.272.39.24617
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Dissociation of the Protein Primer and DNA Polymerase after Initiation of Adenovirus DNA Replication

Abstract: Initiation of adenovirus DNA replication occurs by a jumping back mechanism in which the precursor terminal priming protein (pTP) forms a pTP⅐trinucleotide complex (pTP⅐CAT) catalyzed by the viral DNA polymerase (pol). This covalent complex subsequently jumps back 3 bases to permit the start of chain elongation. Before initiation, pTP and pol form a tight heterodimer. We investigated the fate of this pTP⅐pol complex during the various steps in replication. Employing in vitro initiation and elongation on both n… Show more

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Cited by 26 publications
(35 citation statements)
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“…In this regard, it is informative to compare protein-primed DNA synthesis in hepadnaviruses to that in other viruses (such as the adenovirus and the phage 29) that also employ protein priming to initiate DNA synthesis. Due to the special nature of the protein primer, all protein-primed DNA syntheses studied so far require the polymerase to undergo some structural changes as it switches from an initiation mode, when the primer for DNA synthesis is a protein, to an elongation mode, when the primer for DNA synthesis is the newly formed DNA oligomer (18,21). However, the structural transition from the protein-primed initiation mode to the DNA-primed elongation mode has been shown to occur only after the synthesis of a DNA oligomer of at least several (from 3 to 9) nucleotides in the case of adenoviruses and the bacteriophages (5,18,21).…”
Section: Vol 76 2002 Two Steps In Protein Priming Of Hepadnavirus 5863mentioning
confidence: 99%
“…In this regard, it is informative to compare protein-primed DNA synthesis in hepadnaviruses to that in other viruses (such as the adenovirus and the phage 29) that also employ protein priming to initiate DNA synthesis. Due to the special nature of the protein primer, all protein-primed DNA syntheses studied so far require the polymerase to undergo some structural changes as it switches from an initiation mode, when the primer for DNA synthesis is a protein, to an elongation mode, when the primer for DNA synthesis is the newly formed DNA oligomer (18,21). However, the structural transition from the protein-primed initiation mode to the DNA-primed elongation mode has been shown to occur only after the synthesis of a DNA oligomer of at least several (from 3 to 9) nucleotides in the case of adenoviruses and the bacteriophages (5,18,21).…”
Section: Vol 76 2002 Two Steps In Protein Priming Of Hepadnavirus 5863mentioning
confidence: 99%
“…The transition from initiation to elongation in adenovirus DNA replication is characterized by a jumping-back mechanism, which recovers the terminal three nucleotides, resulting in DNA primer-template elongation and dissociation of pTP (3,4). To study elongation after protein priming, truncated replication reactions were performed in the presence of 50 nM dCTP and additionally 40 M dATP and 40 M dTTP but without dGTP.…”
Section: The (I/y)xgg Motif Of Adenovirus Dna Polymerasementioning
confidence: 99%
“…Ad pol initiates replication opposite the fourth base of the template strand and synthesizes a pTP-CAT intermediate. For elongation to occur, this intermediate jumps back to be paired with template residues 1-3, after which pTP dissociates from Ad pol and elongation starts (3,4). Elongation occurs via a strand displacement mechanism that requires the viral DNAbinding protein (reviewed in Refs.…”
mentioning
confidence: 99%
“…A terminal protein (TP) covalently bound to the 5Ј end of the phage DNA forms a heterodimer with the phage-encoded DNA polymerase, catalyzing covalent linkage of the TP to the first nucleotide of the nascent DNA strand and priming strand-displacing synthesis of full-length 29 DNA (Salas 1991;Salas et al 1995;Mendez et al 1997;Gonzales-Huici et al 2000). In eukaryotic cells, replication of adenoviruses, which also have protein bound covalently to 5Ј DNA termini, occurs by a fundamentally similar proteinprimed strand-displacement mechanism (van der Vliet 1995;Hay 1996;King et al 1997;de Jong and van der Vliet 1999;Liu et al 2000).…”
mentioning
confidence: 99%