Dissolution behavior of silk fibroin in a low concentration CaCl2-methanol solvent: From morphology to nanostructure

Shen, Tingting; Wang, Tao; Cheng, Guotao; Huang, Lang; Chen, Lei; Deng-jun, WU

doi:10.1016/j.ijbiomac.2018.02.022

Cited by 43 publications

(15 citation statements)

References 40 publications

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“…В рамках данного исследования ткани подвергали обработке раствором в течение 45 минут при температуре 37 °С. Обработка водно-спиртовым раствором хлорида кальция на водяной бане используется различными научными группами как одна из стадий получения водного раствора фиброина шелка для осуществления перехода молекул фиброина шелка в состояние α-конформации, которая способствует растворению белка [12,20,21]. Также известно, что скорость деградации конструкций на основе фибро- ина шелка зависит от конформации молекул белка в составе конструкций.…”

Section: Discussionunclassified

Biodegradable materials based on natural silk fabric as promising scaffolds for tissue engineering and regenerative medicine

Safonova¹,

Bobrova²,

Ефимов³

et al. 2021

RJTAO

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ФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России, Москва, Российская Федерация Целью исследования была разработка методики получения скаффолдов на основе ткани из натурального шелка и изучение их биосовместимости in vitro. Для получения биодеградируемых скаффолдов на основе ткани из натурального шелка предложена обработка тканей из натурального шелка водно-спиртовым раствором хлорида кальция. Методом сканирующей электронной микроскопии выявлены различия в структуре полученных скаффолдов. Полученные скаффолды не являются токсичными для клеток, а также поддерживают адгезию и пролиферацию клеток. Проведенные исследования позволяют рассматривать полученные биодеградируемые скаффолды как перспективные конструкции для тканевой инженерии и регенеративной медицины.

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Section: Discussionunclassified

Biodegradable materials based on natural silk fabric as promising scaffolds for tissue engineering and regenerative medicine

Safonova¹,

Bobrova²,

Ефимов³

et al. 2021

RJTAO

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“…Those observations indicate that WPI + MD + LA + CT microparticles demonstrated higher thermal stability compared to WPI + LA + CT and MD + LA + CT microparticles. Moreover, the DSC curves of all microparticles revealed a broad endothermic peak ranging from 30 to approximately 130 • C which was assigned to evaporation of absorbed water [58].…”

Section: Microparticles Thermal Stabilitymentioning

confidence: 98%

Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin

Bora

Lü

2021

Foods

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The identification of novel sources of synbiotic agents with desirable functionality is an emerging concept. In the present study, novel encapsulates containing probiotic L. acidophilus LA-05® (LA) and Charantin (CT) were produced by freeze-drying technique using pure Whey Protein Isolate (WPI), pure Maltodextrin (MD), and their combination (WPI + MD) in 1:1 core ratio, respectively. The obtained microparticles, namely WPI + LA + CT, MD + LA + CT, and WPI + MD + LA + CT were tested for their physicochemical properties. Among all formulations, combined carriers (WPI + MD) exhibited the highest encapsulation yields for LA (98%) and CT (75%). Microparticles showed a mean d (4, 3) ranging from 50.393 ± 1.26 to 68.412 ± 3.22 μm. The Scanning Electron Microscopy revealed uniformly amorphous and glass-like structures, with a noticeably reduced porosity when materials were combined. In addition, Fourier Transform Infrared spectroscopy highlighted the formation of strong hydrogen bonds supporting the interactions between the carrier materials (WPI and MD) and CT. In addition, the thermal stability of the combined WPI + MD was superior to that of pure WPI and pure MD, as depicted by the Thermogravimetric and Differential Scanning Calorimetry analysis. More interestingly, co-encapsulation with CT enhanced LA viability (8.91 ± 0.3 log CFU/g) and Cells Surface Hydrophobicity (82%) in vitro, in a prebiotic-like manner. Correspondingly, CT content was heightened when co-encapsulated with LA. Besides, WPI + MD + LA + CT microparticles exhibited higher antioxidant activity (79%), α-amylase inhibitory activity (83%), and lipase inhibitory activity (68%) than single carrier ones. Furthermore, LA viable count (7.95 ± 0.1 log CFU/g) and CT content (78%) were the highest in the blended carrier materials after 30 days of storage at 4 °C. Synbiotic microparticle WPI + MD + LA + CT represents an effective and promising approach for the co-delivery of probiotic culture and bioactive compounds in the digestive tract, with enhanced functionality and storage properties.

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“…However, only a limited number of solvents are able to dissolve silk fibroin. Examples of these are strong acidic solutions (phosphoric, formic, sulfuric, hydrochloric) or aqueous/organic solutions concentrated in salts (LiCNS, LiBr, CaCl 2 , Ca (CNS) 2 , ZnCl 2 , NH 4 CNS, CuSO 4 , NH 4 OH, Ca(NO 3 ) 2 ) those that are capable of completing the dissolution of fibroin fibers [64][65][66][67][68][69][70]. This is due to the presence of the great network of intra-and intermolecular hydrogen bonds and the high crystallinity derived from its secondary structure.…”

Section: Solubilization Of Silk Fibroinmentioning

confidence: 99%

“…In general, the literature describes the dissolution of fibroin in concentrated salt solutions as a two-step process [64,67]. In the first step, the compact, crystalline structure of fibroin fibers swells due to the diffusion of solvent molecules.…”

Section: Solubilization Of Silk Fibroinmentioning

confidence: 99%

Silk Fibroin Nanoparticles: Synthesis and Applications as Drug Nanocarriers

Carissimi¹,

Montalbán²,

Fuster³

et al. 2022

21st Century Nanostructured Materials - Physics, Chemistry, Classification, and Emerging Applications in Industry, Biomedicine,

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The use of nanoparticles in biomedical fields is a very promising scientific area and has aroused the interest of researchers in the search for new biodegradable, biocompatible and non-toxic materials. This chapter is based on the features of the biopolymer silk fibroin and its applications in nanomedicine. Silk fibroin, obtained from the Bombyx mori silkworm, is a natural polymeric biomaterial whose main features are its amphiphilic chemistry, biocompatibility, biodegradability, excellent mechanical properties in various material formats, and processing flexibility. All of these properties make silk fibroin a useful candidate to act as nanocarrier. In this chapter, the structure of silk fibroin, its biocompatibility and degradability are reviewed. In addition, an intensive review on the silk fibroin nanoparticle synthesis methods is also presented. Finally, the application of the silk fibroin nanoparticles for drug delivery acting as nanocarriers is detailed.

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Dissolution behavior of silk fibroin in a low concentration CaCl2-methanol solvent: From morphology to nanostructure

Cited by 43 publications

References 40 publications

Biodegradable materials based on natural silk fabric as promising scaffolds for tissue engineering and regenerative medicine

Biodegradable materials based on natural silk fabric as promising scaffolds for tissue engineering and regenerative medicine

Physicochemical and Functional Characterization of Newly Designed Biopolymeric-Based Encapsulates with Probiotic Culture and Charantin

Silk Fibroin Nanoparticles: Synthesis and Applications as Drug Nanocarriers

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