2020
DOI: 10.1101/2020.05.18.101659
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Distinct activities of bacterial condensins for chromosome management inPseudomonas aeruginosa

Abstract: Bacteria encompass three types of structurally related SMC complexes referred to as condensins. Smc-ScpAB is present in most bacteria while MukBEF is found in enterobacteria and MksBEF is scattered over the phylogenic tree. The contributions of these condensins to chromosome management were characterized in Pseudomonas aeruginosa that carries both Smc-ScpAB and MksBEF. In this bacterium, SMC-ScpAB controls chromosome disposition by juxtaposing chromosome arms. In contrast, MksBEF is critical for chromosome seg… Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
5
0

Year Published

2020
2020
2022
2022

Publication Types

Select...
2
1

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(5 citation statements)
references
References 49 publications
0
5
0
Order By: Relevance
“…Given the primary function of condensins in chromosome folding, the location of condensin‐dependent genes could be linked to the fine structure of the chromosome. Recently, a DNA interaction map of P. aeruginosa chromosome has been constructed using chromosome conformation capture, Hi‐C (Lioy et al, 2020). In Figure 9, we mapped the genes significantly affected by smc or mksB mutations (>2‐ fold, FDR < 0.1) to the interaction map of the PAO1 chromosome.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Given the primary function of condensins in chromosome folding, the location of condensin‐dependent genes could be linked to the fine structure of the chromosome. Recently, a DNA interaction map of P. aeruginosa chromosome has been constructed using chromosome conformation capture, Hi‐C (Lioy et al, 2020). In Figure 9, we mapped the genes significantly affected by smc or mksB mutations (>2‐ fold, FDR < 0.1) to the interaction map of the PAO1 chromosome.…”
Section: Resultsmentioning
confidence: 99%
“…Hi-C maps of P. aeruginosa (GEO: GSM4560142) were analyzed using MATLAB. The map represents a table of normalized contact frequencies of each 10 kb bin of the PAO1-Orsay genome (Lioy et al, 2020). Each column of the table was fit to a Gaussian distribution, and the width of the distribution used as a measure of the interaction range.…”
Section: Bioinformatic Analysismentioning
confidence: 99%
“…In eukaryotes, the cohesin and condensin SMC complexes are loaded at multiple chromosomal loci, at estimated densities between ~1/200 kb - 1/40 kb and extrude loops of 100’s of kilobases (reviewed in 18 ). In many bacteria, condensin SMC complexes are loaded by the protein ParB primarily at centromeric sequences called parS sites 13–17 . These sites often exist in multiple copies close to one another 20 .…”
Section: Main Textmentioning
confidence: 99%
“…Here, we set up a “crash-course track” system to study what happens when SMC complexes encounter one another. Using the parS /ParB system, which loads SMC complexes in a targeted manner 13–17 , we engineered the Bacillus subtilis chromosome to have multiple SMC loading sites. Chromosome conformation capture (Hi-C) analyses of over 20 engineered strains show an amazing variety of never-before-seen chromosome folding patterns.…”
mentioning
confidence: 99%
“…The simplest model to tackle this issue is the bacterial cell due to its relatively small genome size and chromosome number. The bacterial chromosome is tightly organized within the confined space of the cytoplasm and along the cell cycle (6)(7)(8)(9)(10)(11)(12)(13). This is physiologically relevant since DNA simultaneously serves as template for replication, transcription, segregation and the repair of the genetic material (1,4,14).…”
Section: Introductionmentioning
confidence: 99%