Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

Kirkegaard, Kirstine; Villesen, Palle; Jensen, James C.; Hindkjær, Johnny; Kølvraa, Steen; Ingerslev, Hans Jakob; Lykke‐Hartmann, Karin

doi:10.1016/j.gene.2015.06.057

Cited by 21 publications

(25 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several gene families with similar functions were shared with the Kirkegaard (*) study (Kirkegaard et al 2015), including ATP binding cassette subfamilies (ABCG2, ABCB10*), cytochrome 450 family members, including CYP11A1, CYP51A1, CYP2F1*, and the hydroxysteroid dehydrogenases HSD17B1 and HSD17B11*. A full list of shared gene families is available in Supplemental Table 4.…”

Section: Discussionmentioning

confidence: 99%

“…Two studies in humans attempted to link specific TE RNA profiles and implantation potential following embryo-transfer. The studies employed either multiple (Jones et al 2008) or single (Kirkegaard et al 2015) blastocyst transfers with two possible outcomes recorded (implantation success or failure) per blastocyst transfer. The first study used a microarray-based approach to examine gene expression in 153 samples from either single or pooled blastocyst biopsies.…”

Section: Gene Expression Profiling Of Trophectoderm Biopsiesmentioning

confidence: 99%

See 1 more Smart Citation

Can trophectoderm RNA analysis predict human blastocyst competency?

Ntostis

Kokkali²,

Iles

et al. 2019

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

A systematic review of the literature showed that trophectoderm biopsy could assist in the selection of healthy embryos for uterine transfer without affecting implantation rates. However, previous studies attempting to establish the relationship between trophectoderm gene expression profiles and implantation competency using either microarrays or RNA sequencing strategies, were not sufficiently optimized to handle the exceptionally low RNA inputs available from biopsied material. In this pilot study, we report that differential gene expression in human trophectoderm biopsies assayed by an ultra-sensitive next generation RNA sequencing strategy could predict blastocyst implantation competence. RNA expression profiles from isolated human trophectoderm cells were analysed with established clinical pregnancy being the primary endpoint. Following RNA sequencing, a total of 47 transcripts were found to be significantly differentially expressed between the trophectoderm cells from successfully implanted (competent) versus unsuccessful (incompetent) blastocysts. Of these, 36 transcripts were significantly down-regulated in the incompetent blastocysts, including Hydroxysteroid 17-Beta Dehydrogenase 1 (HSD17B1) and Cytochrome P450 Family 11 Subfamily A Member 1 (CYP11A1), while the remaining 11 transcripts were significantly up-regulated, including BCL2 Antagonist/Killer 1 (BAK1) and KH Domain Containing 1 Pseudogene 1 (KHDC1P1) of which the latter was always detected in the incompetent and absent in all competent blastocysts. Ontological analysis of differentially expressed RNAs revealed pathways involved in steroidogenic processes with high confidence. Novel differentially expressed transcripts were also noted by reference to a de novo sequence assembly. The selection of the blastocyst with the best potential to support full-term pregnancy following single embryo transfer could reduce the need for multiple treatment cycles and embryo transfers. The main limitation was the low sample size (N = 8). Despite this shortcoming, the pilot suggests that trophectoderm biopsy could assist with the selection of healthy embryos for embryo transfer. A larger cohort of samples is needed to confirm these findings.Abbreviations: AMA: advanced maternal age; ART: assisted reproductive technology; CP: clinical pregnancy; DE: differential expression; FDR: false discovery rate; IVF: in vitro fertilization; LD PCR: long distance PCR; qRT-PCR: quantitative real-time PCR; SET: single embryo transfer; TE: trophectoderm

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Gene Expression Profiling Of Trophectoderm Biopsiesmentioning

confidence: 99%

Can trophectoderm RNA analysis predict human blastocyst competency?

Ntostis

Kokkali²,

Iles

et al. 2019

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…Limitations derive from the variability of commercial culture media, high metabolic plasticity of the embryos which can adapt to different culture conditions and from the fact that embryo development and metabolism vary under different culture conditions [300]. Recently, novel strategies based on gene expression profiling of trophectoderm biopsies have been developed and have linked gene expression patterns with developmental competence [301][302][303]. Although complementary approaches may be used to select the best embryos to be transferred, it should be considered that it has been recently proposed that it is the endometrium itself that selectively discriminates between high-and low-quality embryos in order to guarantee a successful implantation.…”

Section: Blastocyst Competence Profiling In Artsmentioning

confidence: 99%

Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk

Massimiani

Lacconi

Civita

et al. 2019

IJMS

135

View full text Add to dashboard Cite

Implantation of the embryo into the uterine endometrium is one of the most finely-regulated processes that leads to the establishment of a successful pregnancy. A plethora of factors are released in a time-specific fashion to synchronize the differentiation program of both the embryo and the endometrium. Indeed, blastocyst implantation in the uterus occurs in a limited time frame called the “window of implantation” (WOI), during which the maternal endometrium undergoes dramatic changes, collectively called “decidualization”. Decidualization is guided not just by maternal factors (e.g., estrogen, progesterone, thyroid hormone), but also by molecules secreted by the embryo, such as chorionic gonadotropin (CG) and interleukin-1β (IL-1 β), just to cite few. Once reached the uterine cavity, the embryo orients correctly toward the uterine epithelium, interacts with specialized structures, called pinopodes, and begins the process of adhesion and invasion. All these events are guided by factors secreted by both the endometrium and the embryo, such as leukemia inhibitory factor (LIF), integrins and their ligands, adhesion molecules, Notch family members, and metalloproteinases and their inhibitors. The aim of this review is to give an overview of the factors and mechanisms regulating implantation, with a focus on those involved in the complex crosstalk between the blastocyst and the endometrium.

show abstract

“…The scoring system is mostly subjective in nature, which makes it difficult to standardize. As a result, the discovery of biomarkers which can be quantified (thus being more objective) has led to focusing on the levels of genomics , transcriptomics , proteomics , and metabolomics . However it must be highlighted that in this moment the morphological evaluation by light microscopy remains the primary approach of embryo assessment during IVF cycles .…”

Section: Introductionmentioning

confidence: 99%

MEKC‐LIF method for analysis of amino acids after on‐capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

et al. 2016

View full text Add to dashboard Cite

Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo's physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo's surroundings can determine the embryo's physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo's surroundings was developed. AAs were analyzed by means of MEKC-LIF after on-capillary derivatization by naphthalene-2,3-dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology. The resulting derivatives of all the standard AAs were baseline resolved in the BGE comprised of 35 mM sodium tetraborate, 55 mM SDS, 2.7 M urea, 1 mM BIS-TRIS propane, and 23 mM NaOH within 50 min. The method was applied on an analysis of spent culture media used in assisted reproduction to culture embryos after in vitro fertilization.

show abstract

Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

Cited by 21 publications

References 65 publications

Can trophectoderm RNA analysis predict human blastocyst competency?

Can trophectoderm RNA analysis predict human blastocyst competency?

Molecular Signaling Regulating Endometrium–Blastocyst Crosstalk

MEKC‐LIF method for analysis of amino acids after on‐capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

Contact Info

Product

Resources

About