Distinct gibberellin functions during and after grapevine bud dormancy release

Zheng, Chuanlin; Acheampong, Atiako Kwame; Shi, Zhaowan; Halaly, Tamar; Kamiya, Yûji; Ophir, Ron; Galbraith, David W.; Or, Etti

doi:10.1093/jxb/ery022

Cited by 70 publications

(46 citation statements)

References 72 publications

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“…Interestingly, we highlighted PavGASA6 and PavGA20ox1, two genes associated with the gibberellin pathway. In particular, PavGA20ox1, that encodes an enzyme required for the biosynthesis of active GA (Plackett et al 2012), is markedly upregulated after endodormancy release, associated with H3K4me3 enrichment at the locus, therefore, suggesting that epigenetic and transcriptomic states facilitate an increase in active GA levels during ecodormancy, as previously shown in hybrid aspen (Rinne et al 2011) and grapevine (Zheng et al 2018).…”

Section: Genes Involved In Bud Dormancysupporting

confidence: 55%

ChIP-seq and RNA-seq for complex and low-abundance tree buds reveal chromatin and expression co-dynamics during sweet cherry bud dormancy

Vimont

Quah

Schoepfer

et al. 2019

Tree Genetics & Genomes

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Chromatin immunoprecipitation-sequencing (ChIP-seq) is a robust technique to study interactions between proteins, such as histones or transcription factors and DNA. This technique in combination with RNA-sequencing (RNA-seq) is a powerful tool to better understand biological processes in eukaryotes. We developed a combined ChIP-seq and RNA-seq protocol for tree buds (Prunus avium L., Prunus persica L Batch, Malus x domestica Borkh.) that has also been successfully tested on Arabidopsis thaliana and Saccharomyces cerevisiae. Tree buds contain phenolic compounds that negatively interfere with ChIP and RNA extraction. In addition to solving this problem, our protocol is optimised to work on small amounts of material. Furthermore, one of the advantages of this protocol is that samples for ChIP-seq are cross-linked after flash freezing, making it possible to work on trees growing in the field and to perform ChIP-seq and RNA-seq on the same starting material. Focusing on dormant buds in sweet cherry, we explored the link between expression level and H3K4me3 enrichment for all genes, including a strong correlation between H3K4me3 enrichment at the DORMANCY-ASSOCIATED MADS-BOX 5 (PavDAM5) loci and its expression pattern. This protocol will allow analysis of chromatin and transcriptomic dynamics in tree buds, notably during its development and response to the environment. Keywords Tree buds. ChIP-seq/chromatin Immunoprecipitation-sequencing. RNA-seq/RNA-sequencing. Prunus avium L.. Prunus persica L batch. Malus x domestica Borkh Key message: We developed a combined ChIP-seq and RNA-seq protocol for tree buds; optimised to work on small amount of material and on samples flash frozen in the field, and explored the link between expression level and H3K4me3 enrichment.

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Section: Genes Involved In Bud Dormancysupporting

confidence: 55%

ChIP-seq and RNA-seq for complex and low-abundance tree buds reveal chromatin and expression co-dynamics during sweet cherry bud dormancy

Vimont

Quah

Schoepfer

et al. 2019

Tree Genetics & Genomes

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“…Along with the chilling accumulation, ABA levels increased, which confirmed a relationship with bud dormancy maintenance [31,32]. While the buds chilling accumulation was enough, endogenous ABA levels were down-regulated and GA 3 level in flower buds were up-regulated during the dormancy release [33][34][35]. Our previous studies showed that hydrogen cyanamide improved GAs:ABA ratio, resulting in the endodormancy release and blooming in sweet cherry [36], and hydrogen cyanamide could down-regulate the expression levels of DAMs in peach [15].…”

Section: Discussionmentioning

confidence: 54%

Dormancy-Associated MADS-Box (DAM) Genes Influence Chilling Requirement of Sweet Cherries and Co-Regulate Flower Development with SOC1 Gene

Wang

Gao

et al. 2020

IJMS

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Floral bud dormancy release of fruit tree species is greatly influenced by climate change. The lack of chilling accumulation often results in the occurrence of abnormal flower and low yields of sweet cherries (Prunus avium L.) in warm regions. To investigate the regulation of dormancy in sweet cherries, six DAM genes with homology to peach DAM, designated PavDAM1-6, have been identified and characterized. Phylogenetic analysis indicate that these genes are similar to DAMs in peach, apple and pear. The expression patterns of the PavDAMs in the low-chill cultivar ‘Royal Lee’ were different from that in the high-chill cultivar ‘Hongdeng’. ‘Royal Lee’ exhibits lower transcriptional level of PavDAM1 compared to ‘Hongdeng’, especially at the stage of chilling accumulation, and transcriptional levels of PavDAM4/5 were high in both cultivars during the endodormancy. Ectopic expression of PavDAM1 and PavDAM5 in Arabidopsis resulted in plants with abnormal flower and seed development, especially the PavDAM5. Higher transcriptional levels of SOC1 were observed in transgenic PavDAM1/5 lines, and ectopic expression of PavSOC1 had the similar floral phenotype. Further, protein interaction analysis demonstrated that PavDAM1/5 could interact with PavSOC1 in vivo and in vitro, which will help clarify the molecular mechanism of the flower development in sweet cherry or other fruit trees.

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“…GA 3 has been shown to modulate the growth and development of plants, mainly by stimulating mitotic division and cell elongation 39,40 . The positive effects of GA 3 on bud break have been reported in the tissue culture of woody plant species [41][42][43][44] . However, adding the improper concentration of GA 3 has a negative effect.…”

Section: Resultsmentioning

confidence: 99%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

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Magnolia sirindhorniae Noot. & Chalermglin is an endangered species with high ornamental and commercial value that needs to be urgently protected and judiciously commercialized. In this study, a protocol for efficient regeneration of this species is standardized. The lateral buds of the M. sirindhorniae plant were used as an explant. Half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 2.0 mg/L gibberellic acid (GA 3 ) was found to be the optimal medium for shoot induction. The maximum shoot multiplication rate (310%) was obtained on Douglas-fir cotyledon revised medium (DCR) fortified with 0.2 mg/L BA, 0.01 mg/L NAA, and additives. The half-strength DCR medium supplemented with 0.5 mg/L NAA and 0.5 mg/L indole-3-butyric acid (IBA) supported the maximum rate (85.0%) of in vitro root induction. After a simple acclimatization process, the survival rate of plantlets in a substrate mixture of sterile perlite and peat soil (1:3; v/v ) was 90.2%. DNA markers were used for assessment of genetic uniformity, confirming the genetic uniformity and stability of regenerated plants of M. sirindhorniae . Thus, the described protocol can safely be applied for large scale propagation of this imperative plant.

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Distinct gibberellin functions during and after grapevine bud dormancy release

Abstract: Gibberellin has an inhibitory effect during initial activation of dormant grapevine buds, and at this stage its level is down-regulated. At a later stage, gibberellin level increases and enhances bud regrowth.

Cited by 70 publications

References 72 publications

ChIP-seq and RNA-seq for complex and low-abundance tree buds reveal chromatin and expression co-dynamics during sweet cherry bud dormancy

ChIP-seq and RNA-seq for complex and low-abundance tree buds reveal chromatin and expression co-dynamics during sweet cherry bud dormancy

Dormancy-Associated MADS-Box (DAM) Genes Influence Chilling Requirement of Sweet Cherries and Co-Regulate Flower Development with SOC1 Gene

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

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