Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

Muravyov, Maxim; Panyushkina, Anna

doi:10.3390/microorganisms8030386

Cited by 18 publications

(15 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acquired metabolic capacity of organic compounds and hydrocarbon in Acidiphilium suggested a mutualistic interaction of autotrophic acidophiles in AMD. For instance, metabolotoxic organic byproducts excreted by autotrophs (e.g., Acidithiobacillus ) might be utilized by chemoorganotrophs (e.g., Acidiphilium ), a process that in turn might stimulate the metabolic processes of the autotrophs ( 78 – 80 ). AMD microbial communities tend to form biofilms on mineral substrates for better metabolic cooperation and enhanced resistance against harsh environments ( 80 – 82 ).…”

Section: Discussionmentioning

confidence: 99%

Insights into the Metabolism and Evolution of the Genus Acidiphilium , a Typical Acidophile in Acid Mine Drainage

Liu

Zhang

et al. 2020

mSystems

View full text Add to dashboard Cite

Here, we report three new Acidiphilium genomes, reclassified existing Acidiphilium species, and performed the first comparative genomic analysis on Acidiphilium in an attempt to address the metabolic potential, ecological functions, and evolutionary history of the genus Acidiphilium. In the genomes of Acidiphilium, we found an abundant repertoire of horizontally transferred genes (HTGs) contributing to environmental adaption and metabolic expansion, including genes conferring photosynthesis (puf, puh), CO2 assimilation (rbc), capacity for methane metabolism (mmo, mdh, frm), nitrogen source utilization (nar, cyn, hmp), sulfur compound utilization (sox, psr, sqr), and multiple metal and osmotic stress resistance capacities (czc, cop, ect). Additionally, the predicted donors of horizontal gene transfer were present in a cooccurrence network of Acidiphilium. Genome-scale positive selection analysis revealed that 15 genes contained adaptive mutations, most of which were multifunctional and played critical roles in the survival of extreme conditions. We proposed that Acidiphilium originated in mild conditions and adapted to extreme environments such as acidic mineral sites after the acquisition of many essential functions. IMPORTANCE Extremophiles, organisms that thrive in extreme environments, are key models for research on biological adaption. They can provide hints for the origin and evolution of life, as well as improve the understanding of biogeochemical cycling of elements. Extremely acidophilic bacteria such as Acidiphilium are widespread in acid mine drainage (AMD) systems, but the metabolic potential, ecological functions, and evolutionary history of this genus are still ambiguous. Here, we sequenced the genomes of three new Acidiphilium strains and performed comparative genomic analysis on this extremely acidophilic bacterial genus. We found in the genomes of Acidiphilium an abundant repertoire of horizontally transferred genes (HTGs) contributing to environmental adaption and metabolic ability expansion, as indicated by phylogenetic reconstruction and gene context comparison. This study has advanced our understanding of microbial evolution and biogeochemical cycling in extreme niches.

show abstract

Section: Discussionmentioning

confidence: 99%

Insights into the Metabolism and Evolution of the Genus Acidiphilium , a Typical Acidophile in Acid Mine Drainage

Liu

Zhang

et al. 2020

mSystems

View full text Add to dashboard Cite

show abstract

“…The chemical composition of the concentrate (iron, arsenic, and other elements) was determined using an inductively coupled plasma atomic emission spectrometer (Optima-4300 DV, PerkinElmer, Waltham, MA, USA) according to the manufacturer’s recommendations. The sulfur content was measured by the gravimetric analysis [ 10 ]. The sulfate concentration was measured turbidimetrically as described [ 8 ].…”

Section: Methodsmentioning

confidence: 99%

“…One of the species, Sb. thermotolerans , has been found to dominate different ACM communities during bioleaching of sulfide raw materials containing gold, silver, zinc, and some other nonferrous metals at 35–45 °C [ 5 , 6 , 7 , 8 , 9 , 10 ]. Sb.…”

Section: Introductionmentioning

confidence: 99%

Understanding Stress Response to High-Arsenic Gold-Bearing Sulfide Concentrate in Extremely Metal-Resistant Acidophile Sulfobacillus thermotolerans

2020

Self Cite

View full text Add to dashboard Cite

Biooxidation of gold-bearing arsenopyrite concentrates, using acidophilic microbial communities, is among the largest commercial biohydrometallurgical processes. However, molecular mechanisms of microbial responses to sulfide raw materials have not been widely studied. The goal of this research was to gain insight into the defense strategies of the acidophilic bacterium Sulfobacillus thermotolerans, which dominates microbial communities functioning in industrial biooxidation processes at >35 °C, against the toxic effect of the high-arsenic gold-bearing sulfide concentrate. In addition to extreme metal resistance, this acidophile proved to be one of the most As-tolerant microorganisms. Comparative proteomic analysis indicated that 30 out of 33 differentially expressed proteins were upregulated in response to the ore concentrate, while the synthesis level of the functional proteins required for cell survival was not negatively affected. Despite a high level of cellular metal(loid) accumulation, no specific metal(loid)-resistant systems were regulated. Instead, several proteins involved in the metabolic pathways and stress response, including MBL fold metallo-hydrolase, sulfide:quinone oxidoreductase, and GroEL chaperonin, may play crucial roles in resistance to the sulfide ore concentrate and arsenic, in particular. This study provides the first data on the microbial responses to sulfide ore concentrates and advances our understanding of defense mechanisms against toxic compounds in acidophiles.

show abstract

“…For bioleaching experiments at 35, 40, and 50 • C, three acidophilic microbial consortia were formed from the stirred-tank biotreatment of the waste containing zinc [25], as well as copper-zinc and copper-nickel concentrates [23]. The original community contained chemolithoautotrophic and mixotrophic strains of iron and sulfur oxidizers assigned to various species: At.…”

Section: Microbial Communities and Cultivation Conditionsmentioning

confidence: 99%

“…During 22 days of bioleaching, the cell populations were monitored using microscopy and metabarcoding analysis at the beginning and the end of the process. Although closely related microorganisms are usually not differentiated only based on the differences in the V3-V4 region, some bacterial and archaeal species were predicted due to the high variability of the V3-V4 regions within the corresponding genera [25]. While the initial cell numbers at 35 and 40 • C were similar (0.8 × 10 9 cells/mL), the original microbial community was more diverse in genera at 40 C. At 50 • C, the cell number was approximately one order of magnitude lower (0.1 × 10 9 cells/mL), and the microbial diversity was the lowest among all three variants (Table 5).…”

Section: Microbial Community Structurementioning

confidence: 99%

Effect of Temperature on Biobeneficiation of Bulk Copper-Nickel Concentrate with Thermoacidophilic Microbial Communities

Panyushkina

Фомченко

Babenko

et al. 2021

Metals

Self Cite

View full text Add to dashboard Cite

Bioleaching of the bulk copper–nickel sulfide concentrate was proposed as a method to remove nickel from it and to obtain a concentrate containing copper as chalcopyrite. This approach is based on the different refractoriness of sulfide minerals in ferric sulfate solutions and oxidation by acidophilic microorganisms. The bulk concentrate contained 10.8% copper in the form of chalcopyrite (CuFeS2) and 7.2% nickel that occurred in pentlandite ((Ni,Fe)9S8) and violarite (FeNi2S4). Three microbial communities grown at 35, 40, and 50 °C were used for bioleaching. The microbial community at 40 °C was the most diverse in the genus and species composition. At all temperatures of the process, the key roles in bioleaching belonged to mixotrophic and heterotrophic acidophiles. The highest levels of nickel leaching of 97.2 and 96.3% were observed in the case of communities growing at 40 and 50 °С, respectively. At the same time, the bioleach residue, which could be characterized as a marketable high-grade copper (chalcopyrite) concentrate, was obtained only at 40 °С. This solid contained 15.6% copper and 0.54% nickel. Thus, the biobeneficiation of bulk sulfide concentrates can be a promising field of biohydrometallurgy.

show abstract

Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

Cited by 18 publications

References 66 publications

Insights into the Metabolism and Evolution of the Genus Acidiphilium , a Typical Acidophile in Acid Mine Drainage

Insights into the Metabolism and Evolution of the Genus Acidiphilium , a Typical Acidophile in Acid Mine Drainage

Understanding Stress Response to High-Arsenic Gold-Bearing Sulfide Concentrate in Extremely Metal-Resistant Acidophile Sulfobacillus thermotolerans

Effect of Temperature on Biobeneficiation of Bulk Copper-Nickel Concentrate with Thermoacidophilic Microbial Communities

Contact Info

Product

Resources

About