Distinct susceptibility and applicability of MDCK derivatives for influenza virus research

Lin, Shih‐Chao; Kappes, Matthew A.; Chen, Mei-Chun; Lin, Chi-Chen; Wang, Tony T.

doi:10.1371/journal.pone.0172299

Cited by 33 publications

(25 citation statements)

References 24 publications

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“…Presence of IAV from nasal swabs was assessed by isolation and propagation of the virus in Madin-Darby canine kidney (MDCK) cells with added trypsin according to standard protocol [ 25 ]. The choice of this technique was based on recent studies that showed that MDCK cell lines proved to be highly sensitive for IAV isolation [ 26 , 27 ]. Virus replication was confirmed based on the cytopathic effect (CPE) produced in the cells and also assessed by hemagglutination assay according to standard protocol [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Longitudinal study of influenza A virus circulation in a nursery swine barn

Ferreira

Grgić

Friendship

et al. 2017

Vet Res

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Commercial production of swine often involves raising animals in large groups through the use of multi-stage production systems. In such systems, pigs can experience different degrees of contact with animals of the same or different ages. Population size and degree of contact can greatly influence transmission of endemic pathogens, including influenza A virus (IAV). IAV can display high genetic variability, which can further complicate population-level patterns. Yet, the IAV transmission in large multi-site swine production systems has not been well studied. The objectives of this study were to describe the IAV circulation in a multi-source nursery facility and identify factors associated with infection in nursery pigs. Pigs from five sow herds were mixed in one all-in/all-out nursery barn, with 81 and 75 pigs included in two longitudinal studies. Virus isolation was performed in Madin-Darby canine kidney cells and serology was performed using hemagglutination inhibition assays. Risk factor analysis for virological positivity was conducted using logistic regression and stratified Cox’s regression for recurrent events. In Study 1, at ≈30 days post-weaning, 100% of pigs were positive, with 43.2% of pigs being positive recurrently over the entire study period. In study 2, 48% of pigs were positive at the peak of the outbreak, and 10.7% were positive recurrently over the entire study period. The results suggest that IAV can circulate during the nursery phase in an endemic pattern and that the likelihood of recurrent infections was associated in a non-linear way with the level of heterologous (within-subtype) maternal immunity (p < 0.05). High within-pen intracluster correlation coefficients (> 0.75) were also observed for the majority of sampling times suggesting that pen-level factors played a role in infection dynamics in this study.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-017-0466-x) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Longitudinal study of influenza A virus circulation in a nursery swine barn

Ferreira

Grgić

Friendship

et al. 2017

Vet Res

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“…MDCK cells modify cell-surface proteins with both α-2,6-linked and α-2,3-linked sialic acids (Ilyushina et al, 2012;Ito et al, 1997;Lin, Kappes, Chen, Lin, & Wang, 2017).…”

Section: Discussionmentioning

confidence: 99%

Chronic exposure to arsenite enhances influenza virus infection in cultured cells

Amouzougan

Lira

Klimecki

2020

J of Applied Toxicology

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Arsenic is a ubiquitous environmental toxicant that has been associated with human respiratory diseases. In humans, arsenic exposure has been associated with increased risk of respiratory infection. Considering the existing epidemiological evidence and the well‐established impact of arsenic on epithelial cell biology, we posited that the effect of arsenic exposure in epithelial cells could enhance viral infection. In this study, we characterized influenza virus A/WSN/33 (H1N1) infection in Madin‐Darby Canine Kidney (MDCK) cells chronically exposed to low levels of sodium arsenite (75 ppb). We observed a 27.3‐fold increase in viral matrix (M2) protein (24 hours postinfection [p.i.]), a 1.35‐fold increase in viral mRNA levels, and a 126% increase in plaque area in arsenite‐exposed MDCK cells (48 hours p.i.). Arsenite exposure resulted in 114% increase in virus attachment‐positive cells (2 hours p.i.) and 224% increase in α‐2,3 sialic acid‐positive cells. Interestingly, chronic exposure to arsenite reduced the effect of the antiviral drug, oseltamivir in MDCK cells. We also found that exposure to sodium arsenite resulted in a 4.4‐fold increase in viral mRNA levels and significantly increased cytotoxicity in influenza A/Udorn/72 (H3N2) infected BEAS‐2B cells. This study suggests that chronic arsenite exposure could result in enhanced influenza infection in epithelial cells, and that this may be mediated through increased sialic acid binding. Finally, the decreased effectiveness of the anti‐influenza drug, oseltamivir, in arsenite‐exposed cells raises substantial public health concerns if this effect translates to arsenic‐exposed, influenza‐infected people.

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“…In this study, entry efficiencies of Scotophilus bat CoV-512 and SARS-CoV in 11 different cells were evaluated using a lentivirus-based pseudovirus system. HEK-293T cells were selected as they have been used for the production of pseudoviruses; additionally, several cell lines were selected because they were used for the isolation and maintenance of viruses in other studies, such as Vero and PK15 cells for PEDV [17], MDCK cells for influenza viruses [18], and Fcwf-4 cells for FCoV [19]. Given that CoVs tend to replicate in the epithelial cells of enteric and respiratory tracts, Caco-2 and IEC-6 were selected.…”

Section: Discussionmentioning

confidence: 99%

Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells

et al. 2019

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Bats are natural reservoirs of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Scotophilus bat CoV-512 demonstrates potential for cross-species transmission because its viral RNA and specific antibodies have been detected in three bat species of Taiwan. Understanding the cell tropism of Scotophilus bat CoV-512 is the first step for studying the mechanism of cross-species transmission. In this study, a lentivirus-based pseudovirus was produced using the spike (S) protein of Scotophilus bat CoV-512 or SARS-CoV as a surface protein to test the interaction between coronaviral S protein and its cell receptor on 11 different cells. Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of Scotophilus bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. Scotophilus bat CoV-512 pseudovirus had significantly higher entry efficiencies in Madin Darby dog kidney epithelial cells (MDCK), black flying fox brain cells (Pabr), and rat small intestine epithelial cells (IEC-6). SARS-CoV pseudovirus had significantly higher entry efficiencies in human embryonic kidney epithelial cells (HEK-293T), pig kidney epithelial cells (PK15), and MDCK cells. These findings demonstrated that Scotophilus bat CoV-512 had a broad host range for cross-species transmission like SARS-CoV.Bats are the natural reservoirs of SARS-CoV, MERS-CoV, and HCoV-NL63. SARS-CoV and HCoV-NL63 gain cellular entry through angiotensin-converting enzyme 2 (ACE2) [9], whereas MERS-CoV utilizes dipeptidyl peptidase-4 (DPP4) as its entry receptor [10]. The entry receptor is considered a hallmark of coronaviral cross-species transmissibility. SARS-related CoV isolated from Rhinolophus bats can use the ACE2 of humans, civets, and Rhinolophus bats as its cell receptor to infect cells originated from human and many other animal species [11]. Both MERS-CoV and bat CoV-HKU4 can use bat and human DPP4 to infect cells originating from humans, camels, bats, and other animal species [12,13] and HCoV-NL63 can replicate in the lung cell line from tricolored bats [8].Little information is available regarding cross-species events for animal CoVs. A previous study detected Scotophilus bat CoV-512 in four different bat species along with Miniopterus bat CoV-1A and Rhinolophus SARS-related CoV in Taiwan through the reverse transcription polymerase chain reaction (RT-PCR) [14]. Antibodies specific to the nucleocapsid (N) protein fragments of Scotophilus bat CoV-512 were detected in serum collected from three bat species, namely Scotophilus kuhlii, Miniopterus fuliginosus, and Rhinolophus monoceros [15]. A close relationship and possible gene recombinants between Scotophilus bat CoV-512 and PEDV were observed through sequence alignments [14,16]. The results of the cell entry assay also indicated that PEDV can infe...

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Distinct susceptibility and applicability of MDCK derivatives for influenza virus research

Cited by 33 publications

References 24 publications

Longitudinal study of influenza A virus circulation in a nursery swine barn

Longitudinal study of influenza A virus circulation in a nursery swine barn

Chronic exposure to arsenite enhances influenza virus infection in cultured cells

Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells

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