Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2

Kalica, Anthony R.; Greenberg, Harry B.; Espejo, Romilio T.; Flores, Jorge; Wyatt, Richard G.; Kapikian, Albert Z.; Chanock, R. M.

doi:10.1128/iai.33.3.958-961.1981

Cited by 129 publications

(73 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eight different profiles were observed and all were typical for Group A rotaviruses (Pedley et al, 1983), except for the C7 type which was typified by the lack of a band in the Segment 11 region and the appearance of a band between Segments 6 and 7. Variation in the position of Segment 11 has been reported for Subgroups 1 and 2 of human Group A rotaviruses (Kalica et al, 1981), although not to the extent seen with the C7 isolates. Rotaviruses with unusual genome profiles have been detected in man and animals (Pedley et al, 1983;Snodgrass et al, 1984), but these isolates have distinct group antigens and their genome profiles lack a triplet of Segments 7, 8 and 9, having similar electrophoretic mobilities, characteristic of Group A viruses.…”

Section: Discussionmentioning

confidence: 93%

Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis

Pocock

1987

Veterinary Microbiology

View full text Add to dashboard Cite

Rotaviruses isolated from 43 sub-clinically infected calves from a single farm were analysed by genome profile analysis. The isolates showed genomic variation and eight different profiles were observed, including one which was atypical for Group A rotaviruses. The 3' terminal labelling method for the analysis of genome profiles used in this study required only 1 ng of viral RNA, an increase of 1000-fold in sensitivity over ethidium bromide staining for detecting all rotavirus genome segments. However, dual infections involving two rotaviruses with distinct profiles could not be detected if the concentrations of the viruses differed by greater than 10-fold.

show abstract

Section: Discussionmentioning

confidence: 93%

Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis

Pocock

1987

Veterinary Microbiology

View full text Add to dashboard Cite

show abstract

“…However, there is no genetic base for this correlation [Garbarg-Chenon et al, 1986;Urasawa et al, 19861. In addition to the two antigenic specificities, subgroup and serotype, which are assigned to the gene products of either RNA segment 6 or of segment 9 (or 8) Kalica et al, 1981;Chanock et al, 19831, there is evidence that Vp3, the product of the 4th gene, has an antigenic specificity involved in the neutralization of rotavirus infectivity [Hoshino et al, 19851. So far, only one HRV strain detected under natural conditions has been reported to have unusual features conflicting with the above rules; a strain called 82A001 with a "long" RNA pattern belonged to subgroup I [Nakagomi et al, 19851. This HRV strain was propagated in tissue culture, designated as AU-1 and confirmed as having unusual genetic and serological features.…”

Section: Introductionmentioning

confidence: 99%

Serologic characteristics of a human rotavirus isolate, AU‐1, which has a “long” RNA pattern and subgroup I specificity

Kitaoka

Nakagomi

Fukuhara

et al. 1987

Journal of Medical Virology

View full text Add to dashboard Cite

An unusual human rotavirus (HRV) isolate, designated AU-1, has a "long" RNA electrophoretic pattern and subgroup I specificity, in contrast to properties common to HRVs previously isolated. Hemagglutination activity of the AU-1 strain was demonstrated using erythrocytes of 1-day-old chicken. Neutralization assays revealed that the AU-1 strain was crossneutralized by hyperimmune antisera against the MO strain (serotype 3). However, antiserum directed against a reassortant in which the gene for Vp7 was replaced by the corresponding AU-1 gene neutralized the AU-1 strain to a significantly higher titer than the MO strain.

show abstract

“…On the basis of differences in the migration of RNA segments on gels, Espejo et al have demonstrated the presence of two distinct RNA patterns among human rotaviruses obtained from different epidemics (1)(2)(3)(4)(5). Most recently, the existence of a correlation between the RNA patterns of human rotaviruses and their serotypes (subgroups) has been demonstrated (6).…”

mentioning

confidence: 99%

“…In collaboration with Dr. A.Z. Kapikian and his colleagues (National Institutes of Health, Bethesda, U.S.A.), 11 rotavirus strains with various RNA patterns were serotyped by the enzyme-linked immunosorbent assay (ELISA) and immune adherence hemagglutination (IAHA) (6,7). The results showed that all the viruses with an RNA pattern of the 8 type should be classified as subgroup I and the viruses with RNA pattern of the L type as subgroup 2 (Table I).…”

mentioning

confidence: 99%

Two Distinct Electrophoretic Migration Patterns of RNA Segments of Human Rotaviruses Prevalent in Japan in Relation to Their Serotypes

Kutsuzawa

Konno

Sano

et al. 1982

Microbiology and Immunology

View full text Add to dashboard Cite

Polyacrylamide gel electrophoretic analysis of viral nucleic acids has revealed II segments of double-stranded RNA patterns in rotaviruses (8). On the basis of differences in the migration of RNA segments on gels, Espejo et al have demonstrated the presence of two distinct RNA patterns among human rotaviruses obtained from different epidemics (1-5). Most recently, the existence of a correlation between the RNA patterns of human rotaviruses and their serotypes (subgroups) has been demonstrated (6).This communication describes the existence of two distinct RNA patterns among rotaviruses obtained from infants hospitalized with acute diarrhea between 1975 and 1980 in Sendai and Yamagata. Eighteen rotavirus strains were isolated and examined in this study. Extraction and purification of the rotaviruses were carried out by the method developed by Espejo et al (I). As a standard strain, the Wa strain of human rotavirus type 2, which was kindly provided by Dr. R.C. Wyatt (National Institutes of Health, U.S.A.) was used. The Wa strain was grown in MAI04 cells. The purified virus was disrupted at 100 C for 2 min in 0.0625 M Tris-HCl, pH 6.8, containing 1% (w/v) sodium dodecyl sulfate, 4 M urea and 5% (v/v) 2-mercaptoethanol. For electrophoresis of the RNA, 10% (w/v) polyacrylamide-0.27% (w/v) bisacrylamide slab gels were prepared. Appropriate amounts of samples were applied to the gels and electrophoresis was carried out at the constant current of 12 m Ajslab gel for 8 hr. The gels were subsequently stained for 15 min with ethidium bromide solution (I !,g/ml in 0.02 M Tris-HCl, pH 7.4) and photographed. Figure 1 shows the RNA gel electrophoretic profiles of rotavirus strains obtained in Sendai (80S1, 80SR002; 80S2, 80SR004) and in Yamagata (80Yl, 80Y003; 80Y2, 80YOOI) in 1980 and the Wa strain. When compared with the RNA pattern of the Wa strain, the most striking difference in mobility was found in RNA segments 10 and II of the 80S1 and 80S2 strains, which moved significantly more slowly than those of the Wa strain. In contrast, as far as RNA segments 10 and 11 are concerned, no difference was found between the Wa strain and 80Yl or 80Y2. The RNA pattern with slower moving 10th and 11th seg-271

show abstract

Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2

Cited by 129 publications

References 10 publications

Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis

Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis

Serologic characteristics of a human rotavirus isolate, AU‐1, which has a “long” RNA pattern and subgroup I specificity

Two Distinct Electrophoretic Migration Patterns of RNA Segments of Human Rotaviruses Prevalent in Japan in Relation to Their Serotypes

Contact Info

Product

Resources

About