Distinctive sequence of human mitochondrial ribosomal RNA genes

Eperon, Ian C.; Anderson, Stephen; Nierlich, Donald P.

doi:10.1038/286460a0

Cited by 177 publications

(93 citation statements)

References 78 publications

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“…Availability of the small subunit RNA sequences of z. mays chloroplasts (Schwarz & Kossel, .1980), P. vulgaris ( Carb9n et al , 1981 , S. cerevisiae (Ruptsov et al, 1980), X. laevis (Salim & Maden, 1981) and various ·mitochondria (Eperon et al, 1980;Van Etten et al, 1980;Sor & Fukuhara, 1980) has made phylogenetic comparisons possible. On the basis of these data, Noller & Woese (1981) and Stiegler et al (1981) have proposed a general secondary structure model for all small subunit RNAs.…”

Section: Introductionmentioning

confidence: 99%

Structure of E. coli 16S RNA elucidated by psoralen crosslinking

Thompson

Hearst

1983

Cell

103

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E. coli 16S RNA in solution was photoreacted With hydro%7Methyltrimethyl-psoralen and long wave ultraviolet light. Positions of crosslinks were determined to high resolution by partially digesting the RNA with r 1 RNase, separating the crosslinked fragme~ts by two-dimensional gel electrophoresis, reversing the crosslink, and sequencing the separated frag-This method yielded the locations of crosslinks to ;t15 nucleotides.Even finer placement has been made on the basis of our knowledge of psoralen reactivity. · Thirteen unique crosslinks were mapped. Seven crosslinks confirmed regions of secondary structure which had been predicted in published phylogenetic models, three crosslinks discriminated between phylogenetic 1110dels, and three proved ~he existence of new structures. The new structures were all long range interactions which appear to be in dynamic equilibrium with local secondary structure. Because this technique yields direct information \ about the secondary structure of large RNAs, it should prove invaluable in studying the structure of other RNAs of all sizes.

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Section: Introductionmentioning

confidence: 99%

Structure of E. coli 16S RNA elucidated by psoralen crosslinking

Thompson

Hearst

1983

Cell

103

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“…No significant homology was found between the 3'-terminal sequences of bacterial 23S rRNA and those of mouse and human mitochondrial 16S rRNAs (16,17). We did not find any significant homology at the level of secondary structure either.…”

Section: Resultsmentioning

confidence: 39%

“…As the 3'-terminal sequences of the large ribosomal rRNAs from yea6t and X.Ztaevi cytoplasmic ribosomes (14,15) and from mouse and human mitochondrial ribosomes (16,17) have recently been sequenced, we checked if these may have common structural features with the 3'-terminus of bacterial 23S rRNAs.…”

Section: Introductionmentioning

confidence: 99%

The 3′-terminal region of bacterial 23S ribosomal RNA: structure and homology with the 3′-terminal region of eukaryotic 28S rRNA and with chloroplast 4.5S rRNA

1981

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The sequence of the 110 nucleotide fragment located at the 3'-end of E.coli, P.vuZgaiz and A.puncata 23S rRNAs has been determined. The homology between the E.coti and P.vutga&ii6 fragments is 90%, whereas that between the E.coti and A.punctata fragments is only 60%. The three rRNA fragments have sequences compatible with a secondary structure consisting of two hairpins. Using chemical and enzymatic methods recently developed for the study of the secondary structure of RNA, we demonstrated that one of these hairpins and part of the other are actually present in the three 3'-terminal fragments in solution. This supports the existence of these two hairpins in the intact molecule. Indeed, results obtained upon limited digestion of intact 23S RNA with T1 RNasewere in good agreement with the existence of these two hairpins.We observed that the primary structures of the 3'-terminal regions of yea6t 26S rRNA and X.&aeviZ 28S rRNA are both compatible with a secondary structure similar to that found at the 3'-end of bacterial 23S rRNAs. Furthermore, both tobacco and wheW chloroplast 4.5S rRNAs can also be folded in a similar way as the 3'-terminal region of bacterial 23S rRNA , the 3'-end of chloroplast 4.5S rRNAs being complementary to the 5'-end of chloroplast 23S rRNA. This strongly reinforces the hypothesis that chloroplast 4.5S rRNA originates from the 3'-end of bacterial 23S rRNA and suggests that this rRNA may be base-paired with the 5'-end of chloroplast 23S rRNA. Invariant oligonucleotides are present at identical positions in the homologous secondary structures of E.Cofi 23S, yea6t 26S , X.Ztaevi 28S and Whew and tobacco 4.5S rRNAs. Surprisingly, the sequences of these oligonucleotides are not all conserved in the 3'-terminal regions of A.putctata or even P.Vuwga&lZ 23S rRNAs. Results obtained upon mild methylation of E.colL 50S subunits with dimethylsulfate strongly suggest that these invariant oligonucleotides are involved in RNA tertiary structure or in RNA-protein interactions.

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“…The location of the major blocks of homology, either between all living systems or only between eukaryotes is summarized in fig.4. All the organelle small ribosomal subunit RNA sequences available so far [32][33][34][35], including mouse mitochondrial 12 S rRNA [35] show several deviations from the 'universally' conserved blocks of sequence depicted in fig.4 (unshaded boxes). When the mouse sequence is compared to the other vertebrate sequence available (Xenopus [ 19]) a striking conservation is observed: only 5 mutations and 1 addition have occurred in the 231 nucleotide sequence (97% homology).…”

Section: Sequence Homologymentioning

confidence: 99%