Distinctiveness and Similarities Between Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated from Cattle and the Community in Israel

Abstract

The goal of this study was to compare the molecular features of bovine- and human community-acquired extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in Israel. Bovine ESBL-producing E. coli were isolated during a point-prevalence study from the main farming locations throughout Israel. Human ESBL-producing E. coli isolates were collected from community-acquired urinary tract infection cases. Molecular typing was done initially by repetitive extragenic palindromic-PCR. Representative isolates were subjected to next-generation sequencing (NGS) and analyzed for multilocus sequence typing (MLST), core genome MLST (cgMLST), bla gene allele, and mobile genetic elements (MGEs) surrounding it. Out of the 287 bovine- and 104 community-derived ESBL-producing E. coli isolates, 44 and 26 isolates were subjected to NGS, respectively. Both populations exhibited a diverse but distinct clonal structure with predominance of several sequence types (STs); two clones, ST-10/167 (n = 13) and ST-38 (n = 8), were present. cgMLST analysis of these clones revealed that the majority of isolates exhibited phylogenetic distance (PD) of >178 gene difference from their closest isolate, with the exception of five isolates that exhibited PD of <24 gene difference, including two bovine- to three community-derived isolates. Hence, clonal transmission of ESBL-producing E. coli between cattle and the community, although uncommon, is likely to have occurred. The bla gene was identified in 52/70 (74%) isolates from both cattle and the community and was surrounded by MGEs that were composed mostly of either the Tn3 or IS1380 families. Thus, MGEs are likely to play an important role in the exchange of resistance genes.

Commonality of Multidrug-Resistant Klebsiella pneumoniae ST348 Isolates in Horses and Humans in Portugal

“…Recent cut-offs advocate that clonal relatedness is accepted for the K. pneumoniae isolates when allele difference are less than 10 (Schürch et al, 2018); using this cutoff we could assume that there is no genetic link between the horse and human isolates. Despite that, other authors have interpreted these cutoffs from a different perspective as they advocate that 16 to 21 allele differences between isolates from carriers from different species suggest that transmission has occurred (Adler et al, 2017; Lifshitz et al, 2018). Moreover, most of the few studies that define cutoffs for cgMLST analysis are based on human isolates only (Schürch et al, 2018) and do not take into account interspecies transmission.…”

Short communication: Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase–producing Escherichia coli clone on a Greek dairy farm

“…However, our finding carries public health implications. First, ESBLproducing E. coli lineages found in food-producing animals have also been found in human infections (Carattoli, 2008;Lifshitz et al, 2018). Moreover, the widespread use of β-lactams in dairy cattle may coselect ESBL-producing colistin-resistant strains, increasing the abundance and mobilization of the mcr-1 gene between strains, or even between bacterial species, potentially increasing human exposure to the gene through various pathways (European Medicines Agency, 2013).…”

Multi-Locus Sequence Typing and Drug Resistance Analysis of Swine Origin Escherichia coli in Shandong of China and Its Potential Risk on Public Health

“…The cluster analysis of swine E. coli isolates showed that the 25 different ST types can be classified into 3 CCs, namely CC10 [ST10 (7) 5), and TDY2 (1)] and ST48 [JN (1)]. The three ST58 swine E. coli strains derived from TDY2 belonged to the group CC155, while the other two ST410 swine E. coli strains derived from TDY2 belonged to the group CC23 (Supplementary Table 4).…”