Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli

Song, Yajun; Yang, Ruifu; Guo, Zhaobiao; Zhang, Minli; Wang, Xiaohui; Zhou, F

doi:10.1016/s0167-7012(99)00123-2

Cited by 56 publications

(37 citation statements)

References 23 publications

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“…Song et al (2000) reported that the highest cellular fatty acid composition of B. licheniformis CCTCC AB 94036 T , B. licheniformis 170513 and B. licheniformis 170514 were anteiso-C15:0 which is very close to our finding. Silva et al (2006) obtained similar results with our findings that nine B. licheniformis strains were close and highest content of anteiso-C15:0 was determined in those strains.…”

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confidence: 91%

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Isolation and identification of alkaline protease producer halotolerantBacillus licheniformis strain BA17

et al. 2007

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An alkaline protease producer Bacillus licheniformis strain was isolated from Van Lake in Turkey. The strain is Gram positive, aerobic, motile, sporulating rod-shaped bacterium. Spores were ellipsoidal and positioned central in nonswollen sporangium. The cells were able to grow well at a pH range of 5.7-10. The optimal growth temperature was found to be 37 °C. Growth at a wide range of NaCl concentration (from 0 to 20%) showed that BA17 is halotolerant. Main fatty acid composition of BA17 was anteiso-C15:0 and iso-C15:0. The strain was presumptively identified as B. licheniformis according to 16S rDNA gene sequence analysis. The most appropriate medium for the growth and protease production is composed of 0.5% yeast extract, 0.5% NaNO 3 , 0.02% MgSO 4 ·7H 2 O, 0.1% K 2 HPO 4 and 0.5% maltose. The optimum temperature and pH of the alkaline protease of strain BA17 were found to be 60 o C and pH 11, respectively. The activity was completely lost in the presence of PMSF, suggesting that the preparation contains serine-alkaline protease(s).

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confidence: 91%

“…The major fatty acid component of BA17, type species of B. licheniformis CCTCC AB94036 T , B. licheniformis 170513 and B. licheniformis 170514 are predominant fatty acids in the genus Bacillus strains examined (Song et al, 2000).…”

Section: Morphological and Physiological Characteristicsmentioning

confidence: 93%

Isolation and identification of alkaline protease producer halotolerantBacillus licheniformis strain BA17

et al. 2007

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“…Some phenotypic characteristics of the novel strains in comparison with strains of several Rhizobium species are shown in Table 1. Growth on LB medium + + + + + 2 + Tolerance of 2 % NaCl 2 2 2 2 + 2 + Growth at 37 u C + + + 2 + 2 2 Growth at pH 10.0 2 2 2 + + 2 2 Utilization of sole carbon sources Aniline-degrading ability + + + 2 2 2 2 A few features differentiate these strains from other Rhizobium species: these strains had the ability to degrade aniline and are resistant to dyes (0.06 % amaranth, 0.038 % crystal violet, 0.070 % reactive brilliant blue X-BR were incubated for 36 h in YMA medium at 28 u C. Sample preparation was performed according to the method described by Song et al (2000). Extraction and analysis of cellular fatty acid methyl esters were carried out as described by Xu et al (2005).…”

Section: Cells Of Strain Dn316mentioning

confidence: 99%

Rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge

Zhang

Ren

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Three aniline-degrading bacteria, strains DN316T , DN316-1 and DN365, were isolated from activated sludge. According to 16S rRNA gene sequence-based phylogenetic analysis, the isolates belonged to the genus Rhizobium, with Rhizobium (5Agrobacterium) radiobacter LMG 140 T as the closest relative, with 96.5 % sequence similarity. Phylogenetic analysis of the representative strain DN316 T using sequences of the glnA, thrC and recA genes and the 16S-23S intergenic spacer region confirmed the phylogenetic arrangement obtained from analysis of the 16S rRNA gene. DNA-DNA relatedness between DN316 T and R. radiobacter LMG 140 T was 43.7 %, clearly indicating that the representative strain DN316 T represents a novel species. Phenotypic and biochemical characterization of the isolates and insertion sequence-PCR fingerprinting patterns showed several distinctive features that differentiated them from closely related species. The major components of the cellular fatty acids were C 18 : 1 v7c (57.10 %), C 16 : 0 (11.31 %) and C 19 : 0 cyclo v8c (10.13 %). Based on our taxonomic analysis, the three isolates from activated sludge represent a novel species of the genus Rhizobium, for which the name Rhizobium borbori sp. nov. is proposed. The type strain is DN316 T (5CICC 10378 T 5LMG 23925 T ).The genus Rhizobium was described by Frank (1889). After a series of taxonomic changes that have been proposed since the 1980s, the genus Rhizobium consists of 43 species at the time of writing, including the recently described species Rhizobium selenitireducens (Hunter et al., 2007), R. pisi (Ramírez-Bahena et al., 2008) García-Fraile et al., 2007;Hunter et al., 2007;Quan et al., 2005).During the course of collection of micro-organisms from environmental sources, three pure bacterial cultures were obtained from aniline-polluted activated sludges. Their taxonomic position was determined by a polyphasic taxonomic analysis, and the results suggest that these strains belong to a novel Rhizobium species.For many years, anilines have been among the most important industrially produced amines. They are used widely in the production of polyurethanes, rubber, azo dyes, drugs, photographic chemicals, varnishes and pesticides (Gheewala & Annachhatre, 1997;Kearney & Kaufman, 1969). Aniline is a harmful substance that pollutes the environment and seriously endangers human health; it has been included on a Chinese blacklist for priority control in environmental protection. Aniline is biodegraded mainly by micro-organisms in soils and water bodies, and many aniline-degrading strains have been reported, such as strains of Delftia (Boon et al., 2001;Liu et al., 2002;Zhang et al., 2008;Liang et al., 2005); Pseudomonas (Meyers, 1992; Fukumori & Saint, 1997;The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene, glnA, thrC, recA and 16S-23S intergenic spacer region sequences of strain DN316 T are respectively EF125187, GQ289384, GQ289386, GQ289385 and EU256385; those for the 16S rRNA gene sequences of strains DN316-1 and DN365 are EU256384 and GU3566...

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“…FAMEs have long been recognized as useful biochemical markers for bacterial classification and characterization (38,53,54). The types and relative abundances of fatty acids produced within a cell are largely determined by an organism's genotype and can be used for identification of different species (50) and strains (34,51) and for discriminating between free spores and vegetative cells (43,47). Commercial systems that streamline fatty acid extraction and detection procedures (42) have facilitated the widespread use of fatty acid profiling to identify bacteria in clinical, agricultural, and biodefense settings (29,53,55).…”

mentioning

confidence: 99%

Use of Fatty Acid Methyl Ester Profiles for Discrimination ofBacillus cereusT-Strain Spores Grown on Different Media

Ehrhardt

Chu

Brown

et al. 2010

Appl Environ Microbiol

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The goal of this study was to determine if cellular fatty acid methyl ester (FAME) profiling could be used to distinguish among spore samples from a single species (Bacillus cereus T strain) that were prepared on 10 different medium formulations. To analyze profile differences and identify FAME biomarkers diagnostic for the chemical constituents in each sporulation medium, a variety of statistical techniques were used, including nonmetric multidimensional scaling (nMDS), analysis of similarities (ANOSIM), and discriminant function analysis (DFA). The results showed that one FAME biomarker, oleic acid (18:1 9c), was exclusively associated with spores grown on Columbia agar supplemented with sheep blood and was indicative of blood supplements that were present in the sporulation medium. For spores grown in other formulations, multivariate comparisons across several FAME biomarkers were required to discern profile differences. Clustering patterns in nMDS plots and R values from ANOSIM revealed that dissimilarities among FAME profiles were most pronounced when spores grown with disparate sources of complex additives or protein supplements were compared (R > 0.8), although other factors also contributed to FAME differences. DFA indicated that differentiation could be maximized with a targeted subset of FAME variables, and the relative contributions of branched FAME biomarkers to group dissimilarities changed when different media were compared. When taken together, these analyses indicate that B. cereus spore samples grown in different media can be resolved with FAME profiling and that this may be a useful technique for providing intelligence about the production methods of Bacillus organisms in a forensic investigation.

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Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli

Cited by 56 publications

References 23 publications

Isolation and identification of alkaline protease producer halotolerantBacillus licheniformis strain BA17

Isolation and identification of alkaline protease producer halotolerantBacillus licheniformis strain BA17

Rhizobium borbori sp. nov., aniline-degrading bacteria isolated from activated sludge

Use of Fatty Acid Methyl Ester Profiles for Discrimination ofBacillus cereusT-Strain Spores Grown on Different Media

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