Distribuição das lesões esquistossomóticas extra-hepáticas em camundongos infectados pelas linhagens BH e SJ do Schistosoma mansoni

Abstract

Foi estudada a distribuição dos granulomas esquistossomóticos no baço, pulmões, rins, coração e intestinos de camundongos parasitados pelas linhagens BH e SJ de S. mansoni. Verificou-se que a distribuição de granulomas produzidos pelos esquistossomos das duas linhagens é semelhante, sendo que a linhagem BH produziu número significativamente maior de lesões no baço e nos pulmões.

Experimental Evaluation of the Pathogenicity of Different Strains ofSchistosoma mansoni

“…The fragments were fixed in aqueous Bouin's solution, and 5 μ m thick histological sections were stained with Masson's trichrome stain. The sections were analyzed with an optical microscope to calculate the number of granulomas per tissue area (1.2469 mm 2 ) based on the method described by Magalhães et al [ 6 ]. The sizes of the granulomatous reactions were determined based on their area.…”

A Study of the Granulomatous Responses Induced by Different Strains ofSchistosoma mansoni

“…The number of granulomas per tissue area (0.984704 mm 2 ) and the extent of the granulomatous response were determined using the techniques described by Magalhães et al [17]. Only granulomas that contained an S. mansoni egg at the center were measured.…”

“…In order to count and measure the schistosome granulomas in the liver (Groups III and IV) and in the lungs (Groups I, II, III, and IV) of the euthanized mice, histological slices (5 μ m thick) were fixed in Bouin's solution, stained with Masson's trichrome and examined using an optical microscope. The number of granulomas per tissue area (0.984704 mm 2 ) and the extent of the granulomatous response were determined using the techniques described by Magalhães et al [ 17 ]. Only granulomas that contained an S. mansoni egg at the center were measured.…”

“…In mice, fewer parasites of the BH strain were necessary to obtain equivalent pathogenesis than those with the SJ strain [ 6 ]. These experiments demonstrated an increased pathogenicity of the BH strain [ 6 , 10 , 17 , 18 ]. In areas that are considered endemic for the BH strain, there are frequent cases of decompensated hepatosplenomegaly, which does not occur in regions where the SJ strain is present.…”

Avaliação da patogenicidade decorrente da infecção pelo Schistosoma mansoni Sambon, 1907, agente de infecções unissexuais em Mus musculus

“…Discordando ainda em parte desses resultados, Magalhães e col. 12 (1979), estudando a distribuição das lesões esquistossomóticas e extra-hepáticas em camundongos infectados pelas linhagens BH e SJ de S. mansoni, verificaram que os segmentos que apresentaram maior número de granulomas eram o íleo, jejuno e ceco.…”