Distribution and biosynthesis of stearidonic acid in leaves of Borago officinalis

Griffiths, Gareth; Brechany, Elizabeth Y.; Jackson, Frances; Christie, William W.; Stymne, Sten; Stobart, A. K.

doi:10.1016/0031-9422(96)00305-6

Cited by 37 publications

(34 citation statements)

References 16 publications

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“…The composition of the leaf lipids with palmitic, α-linolenic and linoleic + linolelaidic acids as the major components and with approximate content of myristic and lauric acids was similar to those stated by Griffiths et al [1996], Sayanova et al [1999] and del Río-Celestino et al [2008]. A level of stearic, oleic + elaidic and palmitoleic acids was much higher in the present research.…”

Section: Discussionsupporting

confidence: 90%

“…Griffiths et al [1996] and del Río-Celestino et al [2008] reported a substantial content of stearidonic acid but this component was not determined in this experiment. Griffiths et al [1996], Sayanova et al [1999] and del Río-Celestino et al [2008] also found a few percentage content of γ-linolenic acid, however this component was not detected in this study. Alike, erucic acid established in very low quantities in the borage leaf lipids by del Río-Celestino et al [2008] was not detected in the present research.…”

Section: Discussioncontrasting

confidence: 76%

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BIOLOGICALLY ACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF BORAGE (Borago officinalis L.) FLOWERS AND LEAVES

Borowy¹,

Chwil²,

Kapłan³

2017

Acta Sci. Pol. Hortorum Cultus

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Recently the interest in borage as a vegetable and medicinal plant has increased, yet the knowledge about a content of biologically active compounds in borage grown in Poland remains very scanty. In the experiment carried out in the south-eastern part of the country, fresh borage flowers and leaves contained 0.02% and 0.12% of lipids with the highest level of palmitic acid, i.e. 44.5% and 33.4%, respectively. Flower lipids contained stearic, oleic, elaidic, linoleic, linolelaidic, arachidonic, myristic and lauric acid as well. Besides, leaf lipids were rich in α-linolenic acid (17.7%), less in palmitoleic acid with absence of arachidonic acid. Leaves were found to have 0.16% of essential oil, 1.0% of flavonoids, 9.2 mg vitamin C·100 g f.w.-1 , 1.9 mg carotenoids·100 g f.w.-1 , 0.77 mg chlorophyll a·1 g f.w.-1 and 0.22 mg chlorophyll b·1 g f.w. -1 . Flowers contained more essential oil and vitamin C while less carotenoids, chlorophyll a and b and flavonoids. 45 components were found in the flower essential oil and among them 16 were identified with cumene (62.9%) as a major one. The content of other components was below 3%. As for the leaf essential oil, 18 compounds were detected, and 5 were identified with the highest level of cumene (58.5%) and hexenyl (13.3%). Generally, flowers had more polyphenols and their ferric reducing ability was higher than that of leaves. Besides, a content of remaining unreduced DPPH radical was higher in the flowers. Notably, the time necessary for 50% reduction of the initial concentration of DPPH radical was 2.5 times longer in flowers, whereas their antiradical efficiency 3 fold lower compared to leaves.

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Section: Discussionsupporting

confidence: 90%

Section: Discussioncontrasting

confidence: 76%

BIOLOGICALLY ACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF BORAGE (Borago officinalis L.) FLOWERS AND LEAVES

Borowy¹,

Chwil²,

Kapłan³

2017

Acta Sci. Pol. Hortorum Cultus

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“…Indeed, all of the data in Table I and the corresponding GC/MS data ( Fig. 2 and data not shown) are consistent with the regioselectivity of the B. napus FAD3 being described as -3 as opposed to the ⌬-15 or ϩ 3 possibilities, which had been suggested previously (Heinz, 1993;Griffiths et al, 1996;Reed et al, 1997).…”

Section: Regioselectivity and Substrate Specificity Of Fad3supporting

confidence: 89%

“…Of particular interest is the 18:3(6,9,12) experiment. Previously, it was found that linseed microsomes were capable of desaturating 18:2(9,12), but not ␥-linolenic acid (18:3[6,9,12]) (Griffiths et al, 1996). In the yeast expression system described herein, the B. napus desaturase appears to catalyze both reactions ( Fig.…”

Section: Regioselectivity and Substrate Specificity Of Fad3mentioning

confidence: 78%

“…Until recently, relatively little was known about the substrate specificity and regioselectivity of this enzyme, which is called variously the extraplastidial (or microsomal or endoplasmic reticulum) linoleate or ⌬-15 or -3 desaturase. It has been suggested that the enzyme measures from the carboxyl end or from an existing double bond (Heinz, 1993;Griffiths et al, 1996), but analysis of hydroxy fatty acid metabolism in developing oilseeds argues against the former and favors either u ϩ x or -3 regioselectivity (Reed et al, 1997).…”

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confidence: 99%

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Characterization of the Brassica napusExtraplastidial Linoleate Desaturase by Expression in Saccharomyces cerevisiae

2000

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The substrate specificity and regioselectivity of the Brassica napus extraplastidial linoleate desaturase (FAD3) was investigated in vivo in a heterologous expression system. A strain of the yeast Saccharomyces cerevisiae producing the plant enzyme was constructed and cultured in media containing a variety of fatty acids. The products of desaturation of these potential substrates were determined by gas chromatographic and mass spectrometric analysis of the yeast cultures. The results indicate that the enzyme has: (a) -3, as opposed to ⌬-15 or double-bond-related regioselectivity, (b) the ability to desaturate substrates in the 16 to 22 carbon range, (c) a preference for substrates with -6 double bonds, but the ability to desaturate substrates with -6 hydroxyl groups or -9 or -5 double bonds, and (d) a relative insensitivity to double bonds proximal to the carboxyl end of the substrate.

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Distribution of fatty acids in edible organs and seed fractions of borage (Borago officinalis L.)

2007

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BACKGROUND: Borage (Borago officinalis L.) is currently used as a vegetable in the north of Spain. The edible parts of the plant are the petioles, leaves and stems. The objective of this study was to determine the oil and fatty acids content in the edible tissues (leaves, petioles and stems) and seed fractions (endosperm + cotyledon, seed coat and elaiosome) of white-and blue-flowered borage accessions.

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Distribution and biosynthesis of stearidonic acid in leaves of Borago officinalis

Cited by 37 publications

References 16 publications

BIOLOGICALLY ACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF BORAGE (Borago officinalis L.) FLOWERS AND LEAVES

BIOLOGICALLY ACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF BORAGE (Borago officinalis L.) FLOWERS AND LEAVES

Characterization of the Brassica napusExtraplastidial Linoleate Desaturase by Expression in Saccharomyces cerevisiae

Distribution of fatty acids in edible organs and seed fractions of borage (Borago officinalis L.)

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