Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia

Ondrušková, Emília; Hečková, Z.; Horáková, Miriam Kádasi; Koltay, A.; Ostrovský, Radovan; Pažitný, Jozef; Adamčíková, Katarína

doi:10.1007/s10658-016-1088-2

Cited by 17 publications

(34 citation statements)

References 24 publications

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“…Square shapes indicate newly confirmed locations in this study, while circles are locations previously published by Ondrušková et al. (). Locations where Dothistroma septosporum , Dothistroma pini or both were identified are marked in blue, red and yellow, respectively…”

Section: Resultssupporting

confidence: 61%

“…The number of positive DNB localities (confirmed by DNA‐based diagnostic tools) in the country doubled. The number of positive DNB sites has increased from 35 (34 P. mugo sites published in Ondrušková et al., ; one P. nigra locality in Barnes, Wingfield, et al., ) to 73. The results indicate that Dothistroma species could be detected across the entire country, wherever the susceptible hosts are present.…”

Section: Discussionmentioning

confidence: 99%

“…Both Dothistroma species (D. septosporum and D. pini) were confirmed by species-specific primer-based PCR on introduced P. nigra, native Pinus sylvestris L. (Barnes, Wingfield, Carbone, Kirisits, & Wingfield, 2014;Barnes et al, 2004; and native P. mugo in planted, ornamental stands (Ondrušková et al, 2017).…”

Section: Introductionmentioning

confidence: 90%

“…at the species level made without molecular methods are not reliable (Barnes, Walla, Bergdahl, & Wingfield, ). Both Dothistroma species ( D. septosporum and D. pini ) were confirmed by species‐specific primer‐based PCR on introduced P. nigra , native Pinus sylvestris L. (Barnes, Wingfield, Carbone, Kirisits, & Wingfield, ; Barnes et al., ; Barnes, Wingfield, et al., ) and native P. mugo in planted, ornamental stands (Ondrušková et al., ).…”

Section: Introductionmentioning

confidence: 94%

“…Fungal cultures were obtained as described previously by Ondrušková et al (2017). Needles were gently surface-sterilized by wiping with a 96% ethyl alcohol-soaked tissue and then placed in damp chambers for 24 hr.…”

Section: Fungal Culture Preparationmentioning

confidence: 99%

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The hosts and geographic range of Dothistroma needle blight in Slovakia

et al. 2018

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Summary The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species‐specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species.

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Section: Resultssupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 94%

Section: Fungal Culture Preparationmentioning

confidence: 99%

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The hosts and geographic range of Dothistroma needle blight in Slovakia

et al. 2018

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Population structure and genetic diversity of Dothistroma septosporum in Slovakia

et al. 2021

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Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection

Jánošíková,

Kobza,

Ondrušková

et al. 2023

Eur J Plant Pathol

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Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables that reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in the susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors.

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Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia

Cited by 17 publications

References 24 publications

The hosts and geographic range of Dothistroma needle blight in Slovakia

The hosts and geographic range of Dothistroma needle blight in Slovakia

Population structure and genetic diversity of Dothistroma septosporum in Slovakia

Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection

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