Population structure and genetic diversity of Dothistroma septosporum in Slovakia

Jánošíková, Zuzana; Dutech, Cyril; Ondrušková, Emília; Adamčíková, Katarína; Mullett, Martin

doi:10.1007/s10658-021-02266-z

Cited by 6 publications

(20 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The exact origin of D. septosporum remains elusive. Population genetic studies using the same set of microsatellite markers across many different studies of individual countries (e.g., Boroń et al, 2021 ; Jánošíková et al, 2021 ; Oskay et al, 2020 ) and intercontinental comparisons have, however, enhanced our understanding of where the pathogen is well established and/or naturalized, and where it has clearly been introduced (Adamson et al, 2018 ; Barnes et al, 2014 ; Drenkhan et al, 2013 ; Mullett et al, 2017 ). Specific pathways of migration, natural range expansions, and anthropogenic introductions are now becoming more evident.…”

Section: Progress In Understanding D Septosporum ...mentioning

confidence: 99%

Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants

Mesarich

Barnes

Bradley

et al. 2023

Molecular Plant Pathology

View full text Add to dashboard Cite

Fulvia fulva and Dothistroma septosporum are closely related apoplastic pathogens with similar lifestyles but different hosts: F. fulva is a pathogen of tomato, whilst D. septosporum is a pathogen of pine trees. In 2012, the first genome sequences of these pathogens were published, with F. fulva and D. septosporum having highly fragmented and near‐complete assemblies, respectively. Since then, significant advances have been made in unravelling their genome architectures. For instance, the genome of F. fulva has now been assembled into 14 chromosomes, 13 of which have synteny with the 14 chromosomes of D. septosporum , suggesting these pathogens are even more closely related than originally thought. Considerable advances have also been made in the identification and functional characterization of virulence factors (e.g., effector proteins and secondary metabolites) from these pathogens, thereby providing new insights into how they promote host colonization or activate plant defence responses. For example, it has now been established that effector proteins from both F. fulva and D. septosporum interact with cell‐surface immune receptors and co‐receptors to activate the plant immune system. Progress has also been made in understanding how F. fulva and D. septosporum have evolved with their host plants, whilst intensive research into pandemics of Dothistroma needle blight in the Northern Hemisphere has shed light on the origins, migration, and genetic diversity of the global D. septosporum population. In this review, we specifically summarize advances made in our understanding of the F. fulva –tomato and D. septosporum –pine pathosystems over the last 10 years.

show abstract

Section: Progress In Understanding D Septosporum ...mentioning

confidence: 99%

Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants

Mesarich

Barnes

Bradley

et al. 2023

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Lately, Jánošíková-Hečková et al (2018) reported the host and geographic range of DNB in Slovakia when both native and planted stands were investigated. The disease was widespread and confirmed in 73 localities in Slovakia, while negative results were recorded in natural and naturally regenerated P. mugo Turra and P. cembra L. stands (Ondrušková et al 2017, Jánošíková-Hečková et al 2018.…”

Section: Introductionmentioning

confidence: 99%

New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization

Adamčíková,

Pastirčáková,

Jánošíková

et al. 2023

AFR

View full text Add to dashboard Cite

Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease were investigated in Slovakia, and new hosts and stand types were identified at the regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_ HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles.

show abstract

“…Both mating types of D. septosporum were identified in Slovakia, with different distributions and ratios in individual localities (Barnes et al, 2014;Ondrušková et al, 2017). The even distribution of both mating types and the high genotypic diversity suggests sexual recombination plays a significant role in the pathogen's life cycle in Slovakia (Jánošíková et al, 2021). The high genetic diversity of D. septosporum suggests the pathogen has been established in Slovakia for a long period of time (Jánošíková et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The even distribution of both mating types and the high genotypic diversity suggests sexual recombination plays a significant role in the pathogen's life cycle in Slovakia (Jánošíková et al, 2021). The high genetic diversity of D. septosporum suggests the pathogen has been established in Slovakia for a long period of time (Jánošíková et al, 2021). Such information has not yet been determined for D. pini.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia

et al. 2021

Self Cite

View full text Add to dashboard Cite

Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species‐specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.

show abstract

Population structure and genetic diversity of Dothistroma septosporum in Slovakia

Cited by 6 publications

References 46 publications

Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants

Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants

New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization

Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia

Contact Info

Product

Resources

About