Distribution and colocalization of NGF and GDNF family ligand receptor mRNAs in dorsal root and nodose ganglion neurons of adult rats

Identification of a Key Motif That Determines the Differential Surface Levels of RET and TrkB Tyrosine Kinase Receptors and Controls Depolarization Enhanced RET Surface Insertion

“…Levels in DRG Neurons-Although RET and TrkB receptors are both expressed in non-peptidergic DRG neurons, direct investigations of their co-expression are rarely reported (20,21). We confirmed the co-expression of RET and TrkB in a subset of P7 rat DRG neurons by immunohistochemical staining (Fig.…”

“…First, we found that RET and TrkB receptors exhibit differential surface levels and identified a key motif (Box1) in the RET juxtamembrane region responsible for this difference. When RET and TrkB were co-expressed in nonpeptidergic DRG neurons (20,21), we found that the cell surface levels of RET were higher than TrkB. An increasing number of studies indicate that receptor trafficking from the biosynthetic pathway to the cell surface is tightly regulated (12,13,31) and that the targeting and sorting of membrane receptors are directed by their intrinsic sequence based signal motifs (32,33).…”

Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity

“…The hub genes in the green module encode BAI1-associated protein 2-like 1 (Baiap2l1, k ME = 0.922) and neurotrophic TRK type 2 (Ntrk2, k ME = 0.911), whereas the MEs include genes for 5-hydroxytryptamine receptor 1D (Htr1d), potassium channel subfamily K member 1 (Kcnk1), Ca 2+ -dependent activator protein for secretion 2 (Cadps2), calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h) and N-terminal EF-hand calcium-binding protein 2 (Necab2). The green module thus represents large neurons expressing Ntrk2 [16]. The purple module contain the parvalbumin gene (Pvalb), a marker of large proprioceptive neurons [38], and the MEs include neurexophilin 1 (Nxph1).…”

“…Defined nociceptor subtypes have been termed C-fiber (C-) or Aδ-fiber (A-) mechanoheat nociceptors (MHNs), mechanical nociceptors (MNs), mechanically insensitive (MI) or sensitive afferents, and mechanoheat-cold nociceptors [5,6]. The molecular properties of DRG neurons have been extensively studied, including their expression of various receptors and ion channels such as neuropeptide Y (NPY) receptors [7], MAS-related G-protein-coupled receptors (MRGPRs) [8,9], voltage-gated Na + channels [10], transient receptor potential (TRP) channels [11,12], ATP receptors [13], acid-sensing ion channels (ASICs) [14,15] and tyrosine kinase receptors (TRKs) [16]. Gene expression profiles of DRG tissue have been also analyzed by microarray and RNA-sequencing (RNA-seq) techniques [17,18].…”

Parasympathetic Innervation and Function of Endocrine Pancreas Requires the Glial Cell Line–Derived Factor Family Receptor α2 (GFRα2)

“…A vast majority of sensory neurons that project into the pancreas are calcitonin gene-related peptide positive (21,22), whereas virtually all spinal sensory neurons that express GFR␣2 are CGRP Ϫ and do not project into the pancreas (M.S.A., unpublished data) (23). In addition, although some sensory neurons in the nodose ganglion project into the islets (24), few nodose ganglion neurons express GFR␣2 (25). Thus, the afferent sensory innervation of the islets is predicted to be intact in the GFR␣2-KO mice.…”