Distribution and Evolution of the Xylanase Genes
 xynA
 and
 xynB
 and Their Homologues in Strains of
 Butyrivibrio fibrisolvens

Dalrymple, Brian P.; Swadling, Yolande; I, Layton; Gobius, Kari S.; Gong, Xue

doi:10.1128/aem.65.8.3660-3667.1999

Cited by 18 publications

(20 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. ¢brisolvens strain H17c(SA) contains a GH family 5 endoglucanase (endI [93]), a GH family 9 cellodextrinase (cedI [94]), a GH family 3 L-glucosidase (bglA [95]) and three xylanase genes (xynB [96], xynE and xynF [89]. B.…”

Section: Butyrivibrio/pseudobutyrivibriomentioning

confidence: 99%

Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics

et al. 2003

View full text Add to dashboard Cite

The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host animal that provides an anaerobic fermentation chamber. Increasing the efficiency with which the rumen microbiota degrades fiber has been the subject of extensive research for at least the last 100 years. Fiber digestion in the rumen is not optimal, as is supported by the fact that fiber recovered from feces is fermentable. This view is confirmed by the knowledge that mechanical and chemical pretreatments improve fiber degradation, as well as more recent research, which has demonstrated increased fiber digestion by rumen microorganisms when plant lignin composition is modified by genetic manipulation. Rumen microbiologists have sought to improve fiber digestion by genetic and ecological manipulation of rumen fermentation. This has been difficult and a number of constraints have limited progress, including: (a) a lack of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c) a poor understanding of which glycolyl hydrolases need to be manipulated, and (d) a lack of knowledge of the functional genomic framework within which fiber degradation operates. In this review the major fibrolytic organisms are briefly discussed. A more extensive discussion of the enzymes involved in fiber degradation is included. We also discuss the use of plant genetic manipulation, application of free-living lignolytic fungi and the use of exogenous enzymes. Lastly, we will discuss how newer technologies such as genomic and metagenomic approaches can be used to improve our knowledge of the functional genomic framework of plant cell wall degradation in the rumen.

show abstract

Section: Butyrivibrio/pseudobutyrivibriomentioning

confidence: 99%

Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics

et al. 2003

View full text Add to dashboard Cite

show abstract

“…1987; Anantharam et al . 1989; Hespell and Whitehead 1990; Hespell and Cotta 1995) and genetic (Amzel and Pedersen 1983; Lin and Thomson 1991; Dalrymple et al . 1999) studies of xylanase expression by this strain.…”

Section: Resultsmentioning

confidence: 99%

Transformation and expression of an anaerobic fungal xylanase in several strains of the rumen bacteriumButyrivibrio fibrisolvens

Gobius

Gong

Aylward

et al. 2002

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: To obtain reliable transformation of a range of Butyrivibrio fibrisolvens strains and to express a Neocallimastix patriciarum xylanase gene in the recipients. Methods and Results: Eight strains (H17c, E14, LP1309, LP1028, AR11a, OB156, LP210B and LP461A) of Bu. fibrisolvens were transformed by the Gram‐positive vector pUB110. A xylanase expression/secretion cassette containing Bu. fibrisolvens promoter and signal peptide elements fused to catalytic domain II of the N. patriciarum xylanase A cDNA (xynANp) was inserted into pUB110 to create the plasmid pUBxynA. pUBxynA was used to transform seven of the Bu. fibrisolvens strains transformed by pUB110. In strain H17c pUBxynA, which produced native xylanase, 2·46 U mg−1 total xylanase activity was produced with 45% extracellular xylanase. In strain H17c pUMSX, 0·74 U mg−1 total xylanase activity was produced with 98% extracellular xylanase. H17c pUBxynA exhibited increased (28·7%) degradation of neutral detergent fibre compared with unmodified H17c; however, progressive loss of pUBxynA was observed in long‐term cultivation. Conclusions: A stable transformation system was developed that was applicable for a range of Bu. fibrisolvens strains and high levels of expression of a recombinant xylanase were obtained in H17c which lead to increased fibre digestion. Significance and Impact of the Study: This stable transformation system with the accompanying recombinant plasmids will be a useful tool for further investigation aimed at improving ruminal fibre digestion.

show abstract

“…By using Prb156 and FP27, we could amplify the 213‐bp target sequence from strain OB156 and a phylogenetically closely related group of B. fibrisolvens consisting of other 9 strains (Table 1), all of which are isolates from the rumen of a deer that form a distinct subgroup (IIb) in group II represented by strain 49 [11,14–16]. No amplification was observed for 19 other Butyrivibrio strains and 13 other rumen bacterial species examined.…”

Section: Resultsmentioning

confidence: 99%

Development of a competitive polymerase chain reaction assay for the ruminal bacteriumButyrivibrio fibrisolvensOB156 and its use for tracking an OB156-derived recombinant

Kobayashi

Forster

Teather

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

A competitive polymerase chain reaction assay targeting the 16S rDNA was developed for quantitating the rumen bacterium Butyrivibrio fibrisolvens OB156. A competitor DNA, serving as an internal control in the competitive polymerase chain reaction reaction, was constructed by polymerase chain reaction using a looped oligo longer than the normal primer. Coamplification of the target DNA with known amounts of the competitor DNA allowed quantitation of the target DNA in both pure culture and mixed culture systems, where minimum quantifiable level of OB156 was 1.7x10(2) and 5.6x10(4) cells, respectively. When an erythromycin-resistant recombinant derived from OB156 was inoculated into a rumen fluid culture, its numbers decreased with time. The rate of decrease measured by the competitive polymerase chain reaction assay was much slower than the rate determined by culture enumeration using erythromycin selection. The competitive polymerase chain reaction assay also showed 48 h persistence of the recombinant at 10(4) ml(-1) even after disappearance of culturable recombinant, suggesting maintenance of the target DNA from uncultivable cells. In an in vivo tracking trial, the recombinant became undetectable within 72 h with either assay, indicating rapid hydrolysis and/or outflow of the cells from the rumen.

show abstract

Distribution and Evolution of the Xylanase Genes xynA and xynB and Their Homologues in Strains of Butyrivibrio fibrisolvens

Cited by 18 publications

References 36 publications

Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics

Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics

Transformation and expression of an anaerobic fungal xylanase in several strains of the rumen bacteriumButyrivibrio fibrisolvens

Development of a competitive polymerase chain reaction assay for the ruminal bacteriumButyrivibrio fibrisolvensOB156 and its use for tracking an OB156-derived recombinant

Contact Info

Product

Resources

About