Distribution and hormonal regulation of membrane progesterone receptors β and γ in ciliated epithelial cells of mouse and human fallopian tubes

Nutu, Magdalena; Weijdegård, Birgitta; Thomas, Peter; Thurin‐Kjellberg, Ann; Billig, Håkan; Larsson, D. G. Joakim

doi:10.1186/1477-7827-7-89

Cited by 64 publications

(48 citation statements)

References 64 publications

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“…Furthermore, we found a weak positive expression for PR in the nuclei and cytoplasm of ciliated cells of the follicular phase, whereas they were negative in the mid-luteal phase. Nutu et al (2009) detected PRβ within the cilia and PRγ at the base of the ciliated cell of the mouse and human oviductal cells. Studies using knockout mice (either PRα or PRβ knockout) described that PRβ does not affect ovarian and uterine response to P4 (Conneely et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Kenngott

Vermehren

Sauer

et al. 2011

J Histochem Cytochem.

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The importance of using techniques that allow the study of pure populations of cells has been increasingly recognized. The authors used laser-assisted microdissection (LAM) in combination with quantitative real-time PCR (qPCR) to assess the relative expression of mRNAs encoding estrogen receptor α (ERα) and progesterone receptor (PR) in the different compartments of the bovine oviduct (epithelium, stroma, smooth muscle coat) during the follicular and mid-luteal phases of the estrus cycle. The localization of receptor mRNA was further studied using non-radioactive in situ hybridization (NISH). A special focus was on whether formalin fixation and paraffin embedding influence the quality and quantity of mRNA obtained from microdissected material. Distinct cyclic changes of the mRNA in the bovine oviduct were observed with elevated levels of PR mRNA transcripts in the epithelium and smooth muscle coat during the follicular phase. The expression of PR mRNA did not vary significantly in the stroma of the bovine oviduct during follicular and mid-luteal phases. In conclusion, the authors found that LAM with qPCR can precisely locate and accurately quantify mRNA expression in specific cell populations from formalin-fixed and paraffin-embedded oviductal tissue.

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Section: Discussionmentioning

confidence: 99%

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Kenngott

Vermehren

Sauer

et al. 2011

J Histochem Cytochem.

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“…These processes are controlled by changing levels of ovarian steroid hormones in the blood, which consequently signifi cantly change the morphology and activity of (not only) the surface epithelial cells of Fallopian tubes. So, all above-mentioned actions have a common denominator -regulation by hormones, the most important, estradiol, progesterone, prostaglandins, oxytocin (Wånggren et al, 2008;Nutu et al, 2009;Kowalik et al, 2013). In the light of this information, the several years old discovery of possible "sensory and pacemaker" function of telocytes inside Fallopian tubes is remarkable.…”

Section: For This Reason Detection Of Telocytes Via Single Antibodiementioning

confidence: 99%

Telocytes (interstitial Cajal-like cells) in human Fallopian tubes

Urban¹,

Miko²,

Kajanova³

et al. 2016

BLL

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BACKGROUND: Telocytes represent a relatively newly discovered population of cells found within the various tissues and organs, including Fallopian tubes. It is presumed that telocytes could serve as a sensor of hormone levels or regulate activity of muscle peristaltic movement. METHODS: Tissue sections from anatomically different parts of Fallopian tubes of 48 women (age 48.8 ± 9.1) were stained for the expression of fi ve different antigens: c-kit (CD117), CD34, vimentin, podoplanin (D2-40) and Dog-1. RESULTS: Telocytes form a network associated with the smooth muscle cells. From the mentioned antibodies, only anti-c-kit (CD117) seems to be relatively selective specifi c to the telocytes, others react also with numerous other cells and tissue structures. Our results when using antibodies against podoplanin and Dog-1 are in dissonance with recent literature -with regards to our results, they are not suitable for detection of telocytes. CONCLUSION: Methods of immunohistochemistry are suitable for identifi cation of telocytes in Fallopian tubes. C-kit (CD117) antigens are useful for routine identifi cation of telocytes in histological sections. This antigen can be combined with CD34 or vimentin in cases of double staining immunohistochemistry (Tab. 1, Fig. 6, Ref. 44). Text in PDF www.elis.sk.

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“…Both classic (cytoplasmic) progesterone receptors [50], as well as membrane-bound progesterone receptor gamma [51] have been implicated to be involved in signal transduction to ciliated cells.…”

Section: Ovarian Steroidsmentioning

confidence: 99%

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

Ezzati

Djahanbakhch

Arian³

et al. 2014

J Assist Reprod Genet

105

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With the advent of assisted reproductive technology in the past three decades, the clinical importance of fallopian tubes has been relatively overlooked. However, successful spontaneous conception requires normal function of the tube to provide not only a conduit for the gametes to convene and embryo to reach the uterine cavity, but also a physiologically optimized environment for fertilization and early embryonic development. In this review, after a brief description of normal human tubal anatomy and histology, we will discuss tubal transport and its principal effectors, including ciliary motion, muscular contractility and tubal fluid. Furthermore, we will discuss the ciliary ultrastructure and regulation of ciliary beat frequency by ovarian steroids, follicular fluid, angiotensin system, autonomic nervous system and other factors such as adrenomedullin and prostaglandins. In the last section, we describe the adverse impact of various pathological conditions, such as endometriosis, infection and smoking on tubal function and ciliary motility.

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Distribution and hormonal regulation of membrane progesterone receptors β and γ in ciliated epithelial cells of mouse and human fallopian tubes

Cited by 64 publications

References 64 publications

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Telocytes (interstitial Cajal-like cells) in human Fallopian tubes

Tubal transport of gametes and embryos: a review of physiology and pathophysiology

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