Distribution and incidence of calcified lesions in DBA/2NCrj and BALB/cAnNCrj mice.

Doi, Kunio; Maeda, Naoyuki; Doi, Chiyo; Isegawa, Naohisa; Sugano, Shigeru; Mitsuoka, Tomotari

doi:10.1292/jvms1939.47.479

Cited by 18 publications

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“…Finally, no influence of SZ-induced diabetes on the development of spontaneous cardiovascular lesions in DBN2N mice (Doi et al, 1985;Maeda, Doi & Mitsuoka, 1986) was detected under the present experimental conditions. More prolonged hyperglycaemia might be necessary for the production of such cardiovascular lesions.…”

Section: Discussioncontrasting

confidence: 44%

“…This suggests that full attention should be paid to histopathological changes in other organs as well as the pancreas when SZ-treated animals are used for studies on diabetes. There are only a few reports, however, on the systemic histopathology of SZ-induced diabetic animals (Arison et al, 1967) generally said that diabetes accelerates the progression of existing cardiovascular lesions in humans.In this paper we report the histopathology of the main organs in SZ-induced diabetic DBA/2N and CD-l mice and the influence of hyperglycaemia on the development of spontaneous cardiovascular lesions in DBAl2N mice (Doi et al, 1985;Maeda, Doi & Mitsuoka, 1986) up to 12 weeks after SZ treatment.Materials and methods 43 female DBA/2NCrj (DBA/2N) mice weighing 10-14 g each and 20 female CRJ:CD-l (CD-I) mice weighing 17-21 g each were obtained from Charles River Japan Inc., Atsugi, Kanagawa, at 4 weeks of age. The mice were housed in an animal room at a temperature of 23 ± 2°C with a relative humidity of 55 ± 5% and were fed CRF-l (Charles River Japan Inc.) and water ad libitum.…”

mentioning

confidence: 99%

“…In this paper we report the histopathology of the main organs in SZ-induced diabetic DBA/2N and CD-l mice and the influence of hyperglycaemia on the development of spontaneous cardiovascular lesions in DBAl2N mice (Doi et al, 1985;Maeda, Doi & Mitsuoka, 1986) up to 12 weeks after SZ treatment.…”

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Histopathology of streptozotocin-induced diabetic DBA/2N and CD-1 mice

Honjo

Doi

et al. 1986

Lab Anim

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SummaryHistopathological examinations were carried out on femaie DBA/2N and CD·l mice which were autopsied 4 and 12 weeks after six daily intraperitoneal injections of streptozotocin (SZ). Histopathological changes related to SZ treatment were found in the pancreas, liver and kidneys. Little difference was observed between the two strains in the histological changes of the pancreas (a decrease in size of the islets, and degranulation and a decrease in the number of B cells) and liver (hypertrophy of hepatocytes and cytoplasmic invagination into hepatocyte nuclei). With regard to the changes in the kidneys, DBA/2N mice showed characteristic inclusions positive to periodic acid-Schiff reagent in the distal tubule epithelial cells, while CD-l mice showed remarkable luminal dilatation and epithelial cell deformation of distal tubules. SZ-induced diabetes had no innuence on the development of spontaneous cardiovascular lesions in DBA/2N mice under the present experimental conditions. Keywords: Mice; Streptozotocin; Diabetes mellitus, experimental; Pancreatic diseases; Liver diseases; Kidney diseasesStreptozotocin (SZ), which was originally developed as an antibiotic and antitumour agent, is now widely used to induce insulin-dependent diabetes mellitus in rodents because of its toxic action on islet B cells (Like et al., 1978). However, it is also known that SZ has acute toxic effects on many organs in some animal species (Levine et al., 1980) and leads to delayed-type renal toxicity in rats (Rakieten et al., 1968;Okawa & Doi, 1983). This suggests that full attention should be paid to histopathological changes in other organs as well as the pancreas when SZ-treated animals are used for studies on diabetes. There are only a few reports, however, on the systemic histopathology of SZ-induced diabetic animals (Arison et al., 1967) generally said that diabetes accelerates the progression of existing cardiovascular lesions in humans.In this paper we report the histopathology of the main organs in SZ-induced diabetic DBA/2N and CD-l mice and the influence of hyperglycaemia on the development of spontaneous cardiovascular lesions in DBAl2N mice (Doi et al., 1985;Maeda, Doi & Mitsuoka, 1986) up to 12 weeks after SZ treatment.Materials and methods 43 female DBA/2NCrj (DBA/2N) mice weighing 10-14 g each and 20 female CRJ:CD-l (CD-I) mice weighing 17-21 g each were obtained from Charles River Japan Inc., Atsugi, Kanagawa, at 4 weeks of age. The mice were housed in an animal room at a temperature of 23 ± 2°C with a relative humidity of 55 ± 5% and were fed CRF-l (Charles River Japan Inc.) and water ad libitum.At 5 weeks of age, 23 DBA/2N and 10 CD-l mice received SZ at 50 mg/kg of bodyweight per day for six consecutive days. SZ (Sigma Chemical Company, St. Louis, MO, USA) was dissolved in citrate buffer (pH 4·5) immediately before daily intraperitoneal injection. The remaining mice of each strain received citrate buffer alone in the same way and served as controls. Bodyweight and food and water intake for 24 h were measured weekly...

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Section: Discussioncontrasting

confidence: 44%

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Histopathology of streptozotocin-induced diabetic DBA/2N and CD-1 mice

Honjo

Doi

et al. 1986

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“…They reported that this relationship does not occur in CBA mice. Doi et al [2) showed the calcification in the heart and aorta of DBA/2NCrj and BALB/cAnNCrj mice and in the tongue of DBA/2NCrj mice and suggested that DBA/ 2NCrj mice were useful models for spontaneous soft tissue mineralization.…”

Section: Discussionmentioning

confidence: 99%

The Activity of Alkaline Phosphatase in the Sera of DBA/2NCrj and CBA/BrA Mice with Calcified Tongue Lesions

Matsushima

Ikeno²

1987

Experimental Animals

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Spontaneously occurring calcified lesions were found in the tongues of DBA/2NCrj and CBA/BrA mice. In the DBA/2NCrj strain, the frequency of the lesion was 80% (males) and 88% (females). The youngest age of a mouse with this lesion was 18 days after birth, and 3-4 lesions were found in the tongue of 6-to 8-week-old mice. In CBA/BrA mice, 20% of females and 48% of males had the lesions. No significant differences were found in the serum calcium concentrations in high and low lesiondeveloping strains, but the alkaline phosphatase activities in the high-developing DBA/ 2NCrj, DBA/LiA, and CBA/BrA strains were higher than in strains with no calcified lesions.

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“…The intact hearts were also stored in formol. Dystrophic cardiac calcification in mice is often associated with calcification of other tissues (Doi et al 1985). To further characterize the mice used, lungs, stomach, left kidney, tongue, diaphragm and femoral extensor muscles were excised from selected mice and stored in formol until histological examination.…”

Section: Dissectionmentioning

confidence: 99%

Co-variance of chemically and histologically analysed severity of dystrophic cardiac calcification in mice

Broek¹,

Beems

Tintelen

et al. 1997

Lab Anim

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SummaryDystrophic cardiac calcification (DCC) is a post-mortem finding in mice of various strains frequently used in biomedical research. The major aim of this study was to see whether DCC severity can be assessed by chemical analysis of calcium in the heart. Histological examination was used as the method of reference. Hearts of mice of four strains (BALB/c,C3H, C57BL/6 and DBA/2) were halved and the two resulting parts were subsequently subjected to histology or chemical analysis. Within hearts, the halves generally yielded similar results. The DCC scores and calcium contents were directly correlated within hearts. Thus, calcium analysis could serve as an alternative to histological examination in the assessment of DCC severity in mice.DBA/2 and C3H mice were found to be affected by DCC. Plasma magnesium concentrations were lower in these strains than in the DCC-free C57BL/6 and BALB/cstrains. The tongue, lungs and diaphragm were also found to be calcified in DCC positive animals. Possibly, DCC is just one component of a generalized soft tissue calcification.

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Distribution and incidence of calcified lesions in DBA/2NCrj and BALB/cAnNCrj mice.

Cited by 18 publications

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Histopathology of streptozotocin-induced diabetic DBA/2N and CD-1 mice

Histopathology of streptozotocin-induced diabetic DBA/2N and CD-1 mice

The Activity of Alkaline Phosphatase in the Sera of DBA/2NCrj and CBA/BrA Mice with Calcified Tongue Lesions

Co-variance of chemically and histologically analysed severity of dystrophic cardiac calcification in mice

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