Distribution and Metabolism of I131 Labeled Human Serum Albumin in Congestive Heart Failure With and Without Proteinuria

Albumin and gamma-globulin tracer studies in protein depletion states.

“…In plasmapheresis experiments,-on the other hand, we confirmed the finding of Matthews (10) that this rate was greatly increased. Matthews (12) (34) did not find increased albumin synthesis in a series of patients with albuminuria and regarded decreased catabolism as the major compensatory reaction to the urinary loss. In contrast, Blahd, Fields, and Goldman (41) found increased albumin production in the nephrotic syndrome when dietary protein intake was high.…”

“…Waterlow (30) has questioned whether changes in protein metabolism vary with the level of protein intake at the time of the test or with the state of depletion; he concluded that both factors play a part in determining albumin catabolism. Reduction of dietary protein is obviously not the only cause of low albumin catabolic rates, since they are found in human subjects with proteinuria (34) or cirrhosis of the liver (7) as well as in experimental animals subjected to plasmapheresis, where ad libitum feeding is permitted [(10) and our own study]. In our series of hypoalbuminemic subjects (Table I) (34) and Friedberg (35) appears to be more closely related to reduction in the albumin pool than to protein intake.…”

“…Reduction of dietary protein is obviously not the only cause of low albumin catabolic rates, since they are found in human subjects with proteinuria (34) or cirrhosis of the liver (7) as well as in experimental animals subjected to plasmapheresis, where ad libitum feeding is permitted [(10) and our own study]. In our series of hypoalbuminemic subjects (Table I) (34) and Friedberg (35) appears to be more closely related to reduction in the albumin pool than to protein intake. Our study B provides further evidence that this is so, as these subjects showed the lowest catabolic rates in the third week, when the dietary protein intake, although low, was actually higher than in the second week; this seems to reflect the continued fall in albumin pool size.…”

Insulin-I131 Metabolism in Human Subjects: Demonstration of Insulin Binding Globulin in the Circulation of Insulin Treated Subjects 1

“…The methods developed during this study have permitted quantitative evaluation of the rate of insulin-I'13 metabolism and have led to the discovery of an insulin-transporting globulin in the blood of insulin treated subjects. Regular crystalline insulin 4 was iodinated by the method of Pressman and Eisen (3) (Method A) or by Newerly's modification of their method as previously described (4) (Method B). Insulin-I13.…”

“…The radioactivity bound to serum proteins disappears from plasma much more slowly than unaltered insulin-Il31, so that within a short period of time following intravenous administration most of the protein precipitable radioactivity in plasma is not insulin-Il31. 4. During paper electrophoresis of plasma containing insulin-Ils', the labeled insulin is adsorbed to the paper at the site of application, thus permitting separation from plasma protein bound radioactivity.…”