Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype

Yamasaki, Yoshie; Nomura, Ryota; Nakano, Kazuhiko; Inaba, Hiroaki; Kuboniwa, Masae; Hirai, Norio; Shirai, Mitsuyuki; Kato, Yukio; Murakami, Masaru; Naka, Shuhei; Iwai, Shinichi; Matsumoto‐Nakano, Michiyo; Ooshima, Takashi; Amano, Atsuo; Asai, Fumitoshi

doi:10.1016/j.vetmic.2012.07.026

Cited by 14 publications

(34 citation statements)

References 18 publications

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“…Periodontal condition was evaluated by measuring several parameters of a representative tooth (mandibular left canine), as previously described [13]. Briefly, periodontal pocket depth was measured to the nearest millimeter around the circumference of each tooth from the gingival margin to the deepest probing point, using a round-ended probe tip 0.4 mm in diameter, with the maximum value recorded.…”

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Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs

Hirai

Shirai

Kato

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. We analyzed the distribution of 11 periodontitis-related bacterial species in dental plaque collected from 176 Japanese dogs divided into young (less than 2 years of age), middle-aged (2-7 years of age) and elderly (more than 8 years of age) groups using a polymerase chain reaction method. Clinical examination revealed that no dogs in the young group were affected by periodontitis, whereas the rates for gingivitis and periodontitis were high in the middle-aged and elderly groups. In addition, the total numbers of bacterial species in the middle-aged and elderly groups were significantly greater than in the young group. Our findings suggest that age is an important factor associated with the distribution of periodontitis-related bacteria and periodontal conditions in dogs. KEY WORDS: age, canine, dental plaque, periodontitis, periodontitis-related bacteria.doi: 10.1292/jvms.13-0041; J. Vet. Med. Sci. 75 (7): [999][1000][1001] 2013 Periodontal diseases are quite commonly identified in dogs. The diseases are composed of gingivitis, a reversible condition that features limited inflammation of gingival tissue, and periodontitis, which indicates destruction of periodontal tissues, such as the cementum, periodontal ligaments and supportive bone [8]. It has been summarized that the distribution frequency of gingivitis in dogs aged from 0 to 14 years old (average; approximately 6 years old) ranges from 95-100%, while that of periodontitis is from 50-70% [3].Our previous study analyzed the distribution of 10 human periodontitis-related species in dogs and found that specific species, such as Tannerella forsythia and Campylobacter rectus, were frequently detected [5]. In addition, Porphyromonas gulae, a major pathogen of periodontitis in dogs [2], was also detected at a high frequency [5]. On the other hand, the detection rates of other species, including Porphyromonas gingivalis, Treponema denticola, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans and Eikenella corrodens, were quite low [5]. In addition, several periodontopathic species were shown to be possibly transmitted between dogs and their owners, although the distribution of periodontopathic species in both is generally different [12].In the present study, to investigate the influence of the age of dogs on the periodontal conditions and the distribution of the periodontitis-related species, 176 Japanese dogs were analyzed. On the basis of the age classifications of young (less than 2 years of age), middle-aged (2-7 years of age) and elderly (more than 8 years of age) dogs [6], the 176 dogs were classified into the young (n=39), middle-aged (n=62) and elderly (n=75) groups. The protocols used in this study were approved by

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Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs

Hirai

Shirai

Kato

et al. 2013

The Journal of Veterinary Medical Science

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“…PCR amplification was performed in a total volume of 20 μl with 1 μl of bacterial DNA using Ex Taq polymerase (Takara Bio, Shiga, Japan) with the following cycling parameters: initial denaturation at 95°C for 4 minutes and then 30 cycles consisting of 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds, with a final extension at 72°C for 7 minutes. Specificity with this method was shown in our previous study, with the minimum detection level reported to be 10-100 cells Yamasaki et al, 2012). The dominant genotypes were determined using samples with multiple fimA genotypes detected.…”

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confidence: 88%

“…Detection of P. gulae and fimA genotypes in addition to Tannerella forsythia and Campylobacter rectus were performed by PCR using speciesspecific sets of primers (Kato et al, 2011;Nomura et al, 2012;Yamasaki et al, 2012). PCR amplification was performed in a total volume of 20 μl with 1 μl of bacterial DNA using Ex Taq polymerase (Takara Bio, Shiga, Japan) with the following cycling parameters: initial denaturation at 95°C for 4 minutes and then 30 cycles consisting of 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds, with a final extension at 72°C for 7 minutes.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…The cell surface component Fimbrillin (FimA), a 41-kDa fimbriae subunit, of P. gulae has also been characterized and shown to be a possible factor related to periodontal disease in dogs (Hamada et al, 2008). In our recent study, FimA of P. gulae was classified into 3 major genotypes; A, B, and C, with type C shown to have a high virulence for periodontal disease, followed by B and A Yamasaki et al, 2012).…”

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Short communication: Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation

Shirai

Nomura

Kato

et al. 2015

Research in Veterinary Science

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“…Based on its nucleotide sequence variation, the fimA gene has been classified into six types (I, Ib, II, III, IV, V) (99,100). fimA genotypes II, Ib, and IV were shown to cause inflammatory changes in animal models (101,102,103).…”

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confidence: 99%

Tobacco-gene activity profile in Porphyromonas gingivalis, Filifactor alocis and Treponema denticola.

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Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype

Cited by 14 publications

References 18 publications

Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs

Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs

Short communication: Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation

Tobacco-gene activity profile in Porphyromonas gingivalis, Filifactor alocis and Treponema denticola.

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