Distribution and molecular diversity of three cucurbit-infecting poleroviruses in China

Shang, Qiaoxia; Xiang, Hai-Ying; Han, Chenggui; Li, Dawei; Yu, Jialin

doi:10.1016/j.virusres.2009.07.017

Cited by 41 publications

(32 citation statements)

References 18 publications

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“…This important percentage reflected the high incidence of CABYV in the fields (Table 1), and demonstrated the prevalence of CABYV in squash-growing fields of Egypt. Such a high incidence of CABYV was reported also in other countries like Lebanon, Spain, Tunisia and China (Abou-Jawdah et al 2000;Kassem et al 2007;Mnari-Hattab et al 2009;Shang et al 2009). In accord with our observations, all these regions had an abundance of aphids in open fields.…”

Section: Resultssupporting

confidence: 64%

Cucurbit aphid-borne yellows virus in Egypt

Omar

Bagdady

2012

Phytoparasitica

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During 2010, yellowing symptoms were frequently observed in cultivated squash fields in Egypt. A total of 717 symptomatic squash leaf samples were collected from four regions where squash cultivation is of economic importance for the country: Kafrelsheikh, El-Behira, El-Sharkia and El-Ismailia. Serological analysis showed that 95.6% of the symptomatic squash samples were infected by Cucurbit aphid-borne yellows virus (CABYV), and visual estimation of the incidence of yellowing symptoms suggested a very high incidence of CABYV in the fields. Twelve CABYV isolates were characterized by sequencing two regions of the viral genome, open reading frame (ORF) 3 and ORFs 4/5. Overall, Egyptian isolates were very similar among them, and had higher similarity values with a French than with a Chinese isolate. The average nucleotide diversity for ORF 3 was significantly higher than for the other two regions, indicating that variability is not evenly distributed along the viral genome. The ratios between nucleotide diversity values in non-synonymous (d N ) and synonymous (d S ) positions (d N /d S ) for each ORF showed that the three ORFs are evolving under different pressures, although predominantly under purifying selection. Phylogenetic analyses revealed that these Egyptian isolates, with only one exception, shared the same clade with a French isolate. Moreover, these analyses suggested that Egyptian isolates belong to the Mediterranean group described previously.

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Section: Resultssupporting

confidence: 64%

Cucurbit aphid-borne yellows virus in Egypt

Omar

Bagdady

2012

Phytoparasitica

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“…The isolate used in the current study was then designated the Saudi Arabian isolate of WMV (WMV-SA). Molecular methods had been used in the identification and characterization of WMV and other Potyviruses and cucurbits-infecting viruses in plants and their aphid vectors in earlier studies [27][28][29][30][31][32][33].…”

Section: Serological Proprieties Of Wmvmentioning

confidence: 99%

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Santosa¹,

Al-Shahwan²,

Abdalla³

et al. 2018

JAST-A

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In one of the field visits to cucurbits-growing areas in vicinity of Riyadh city during 2013-2015, severe virus disease-like symptoms were observed on watermelon in Al-Ammariyah area. Mechanical inoculation of the different plant species used in the host range study, from the collected symptomatic watermelon samples, produced mosaic symptoms on Citrullus lanatus, Cucumis sativus, Cucurbita pepo, C. melo, C. melo subsp. melo and Nicotiana benthamiana, but chlorotic local lesions on Chenopodium amaranticolor. No symptoms were observed on the rest of the other inoculated plant species. The virus was transmitted by Aphis gossypii and A. craccivora in a non-persistent manner. Transmission electron microscopic examination of watermelon samples using the leaf dip method revealed only microscopic filamentous shaped virus particles measuring 750 nm in length and 12 nm in diameter in average. ELISA revealed positive results only to Watermelon mosaic virus (WMV) and negative to Zucchini yellow mosaic virus (ZYMV), Papaya ringspot virus (PRSV), Cucumber mosaic virus (CMV) and Squash mosaic virus (SqMV). Specific bands of approximately 825 bp were formed on agarose gel following electrophoresis of the reverse transcriptase-polymerase chain reaction (RT-PCR) products of each of the naturally infected C. lanatus, and artificially infected C. lanatus, C. pepo, C. sativus, and N. benthamiana. The homology tree that was constructed from multiple sequence alignments of the detected Saudi Arabian isolate of WMV (WMV-SA) with 18 other isolates of WMV from nine different countries indicated close relationships between them. Two isolates from Spain and two other isolates from Iran were more closely related to the WMV-SA whereas the isolate from Poland was the least.

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“…Various plant viruses infecting cucurbits cause significant economic losses, which has become a limiting factor for the production of cucurbits [1][2][3]. Cucurbit aphid-borne yellows virus (CABYV), Melon aphid-borne yellows virus, (MABYV) and Suakwa aphid-borne yellows virus (SABYV) are important viruses infecting cucurbits, belonging to the genus polerovirus, the family Luteoviridae [4][5][6]. CABYV was first reported to infect cucurbit crops in France in 1992 and later found in many other countrie including China [4,[7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, CABYV can also infect passion fruit and pepper naturally, which causes a large area of yellowing symptoms in fields [15][16][17]. MABYV and SABYV were first reported in China in 2008 and 2009, respectively [5,6]. MABYV is widely distributed in many provinces in China and Thailand; however, SABYV has been only detected in coastal provinces such as Fujian and Guangdong in southern China [2,6].…”

Section: Introductionmentioning

confidence: 99%

Development of polyclonal antisera against movement proteins of the three poleroviruses infecting cucurbits and their serological relationships

Zhang¹,

Zhao²,

Shi³

et al. 2020

Preprint

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Background: Cucurbit aphid-borne yellows virus (CABYV), Melon aphid-borne yellows virus (MABYV) and Suakwa aphid-borne yellows virus (SABYV) are three critical viruses infecting cucurbit crops. The preparation of specific antiserum against the virus is crucial for both the detection of virus and understanding the functions of the related genes. However, there is no report about detecting the three viruses using antisera against movement proteins (MP). Methods: In this study, we constructed prokaryotic expression vectors of the three viral movement proteins and transferred them into Escherichia coli strain Rosetta to purify the fusion proteins. Then the polyclonal antisera were obtained by immunizing New Zealand white rabbits. Western blotting was used to demonstrate the applicability of the three antisera. Results: We discovered that the titer of antiserum against MP CABYV reached to 1: 512000, and the titers of antisera against MP MABYV and MP SABYV reached to 1:256000. The optimized working concentration range for the three antisera was from 1:10000 to 1:64000. Both antisera against MP CABYV and MP MABYV could only react with the corresponding MP. The antiserum against MP SABYV not only had the strongest reaction with its MP but also could react with MP CABYV and MP MABYV at relative weaker levels and all the three antisera had no serological reactions with other poleroviruses tested. Furthermore, our results showed that the three antisera could specifically detect movement proteins both in Nicotiana benthamiana and cucumber leaves. Conclusions: We have established a sensitive system for detecting three poleroviruses infecting cucurbits by antisera against movement proteins, providing a material foundation for the future research on both the serological detection of viruses and the interaction mechanisms between the virus and host plants.

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Distribution and molecular diversity of three cucurbit-infecting poleroviruses in China

Cited by 41 publications

References 18 publications

Cucurbit aphid-borne yellows virus in Egypt

Cucurbit aphid-borne yellows virus in Egypt

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Development of polyclonal antisera against movement proteins of the three poleroviruses infecting cucurbits and their serological relationships

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