Distribution of 37 human papillomavirus types in parotid gland tumor tissues

Abstract

Human papillomavirus (HPV) infection has been shown to be associated with human tumorigenesis. The aim of the present study was to demonstrate the association between HPV infection and parotid gland tumors. Paraffin-embedded tissue sections from 59 cases of parotid gland tumors and 20 normal oral mucosa were subjected to DNA extraction and flow-through hybridization and gene chip technology to detect infection of 37 HPV types. The HPV-positive rate was 57.6% in parotid gland tumor paraffin-embedded tissue specimens, whereas, the normal control group was negative for HPV. The HPV-positive rate was 59.6% in parotid gland benign tumor tissues and 42.9% in parotid malignant tissues. HPV infection in parotid gland tumors was dominated by the high-risk subtypes (80.7%), which mainly consisted of HPV 16, 18 and 52 (61.4%). In addition, parotid gland tumor tissues were found to be infected by multiple or single types of HPV, but were predominantly infected by mixed HPV types. In this study, we found that the occurrence of parotid gland tumor is correlated with HPV infection.

Distribution characteristics of different human papillomavirus genotypes in women in Wuhan, China

“…This method, called HybriMax, can be used to simultaneously identify 21 different HPV genotypes, including 14, 5, and 2 genotypes of HR‐HPVs, LR‐HPVs and unknown‐risk HPVs, respectively. This identification is particularly useful for detecting multiple or coinfections of different HPV genotypes, and HybriMax is a reasonable or even ideal technique for performing HPV genotyping in a clinical setting …”

“…All the DNA samples were amplified using the Real‐Time PCR system ABI 7500 (Applied Biosystems, USA) using a PCR reagent kit (Chaozhou Hybribio Biochemical Co., Ltd.). HPV genotyping was performed with a HybriMax HPV GenoArray Test Kit (HybriBio Ltd., Chaozhou, China) according to the manufacturer’s instructions . Positive and negative controls were run in each PCR analysis process to control for possible contamination and accuracy.…”

A proportion of primary squamous cell carcinomas of the parotid gland harbour high‐risk human papillomavirus

“…showed that HPV DNA was absent in Warthin's tumour, a benign salivary epithelial neoplasm. In contrast, Teng et al . detected the presence of HR‐HPVs, including HPV16 and HPV18, in four of 12 (33%) Warthin's tumours.…”

“…detected the presence of HR‐HPVs, including HPV16 and HPV18, in four of 12 (33%) Warthin's tumours. Similarly, the reported incidence of HR‐HPV in mucoepidermoid carcinoma, the most common malignant salivary gland epithelial tumour, varied from 0% to 47–100% . With regard to ParSCC, the only case with a documented HPV status was reported by Fischer and Von Winterfeld, who detected the presence of HPV in a single case of ParSCC.…”

“…A handful of published studies have investigated the effects of HPV, including HR-HPVs, in epithelial neoplasms, benign or malignant, of salivary glands. [7][8][9][10][11][12][13][14][15] The results were somewhat inconsistent, with a documented incidence of HPV ranging from 0% to 100%. In addition, the majority of prior published studies have employed polymerase chain reaction (PCR)-based assays.…”