Distribution of a Guinea Pig Lymphocyte Cell Surface Antigen of Defined Function, as Detected by the Mouse Monoclonal Antibody MSgp 1

Healey, Dan; Parker, Darien; Turk, J.L.

doi:10.1159/000234175

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…A further immunization with 2 x 10s cells obtained as above was given via the same route on day 14. Five days later, the sensitized spleen was excised and the splenocytes fused to NS-1 mouse myeloma cells (Flow Laboratories) as previously described [Healey, et al, 1987]. The resultant hybridomas were screened by immunocytochemistry on normal guinea pig lymph node sections and positive hybridomas cloned by limiting dilution.…”

Section: Mitogenmentioning

confidence: 99%

Accessory Cell Function of Dendritic Cells from Lymph Nodes containing <i>Mycobacterium leprae</i> Induced Granulomas

Verghese

Healey

Curtis

et al. 1988

Int Arch Allergy Immunol

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Dendritic cells were enriched from guinea pig auricular lymph nodes containing Mycobacterium leprae induced granulomas by immunomagnetic depletion of other cells. These cells were strongly positive for major histocompatibility complex class II antigens and labelled with an antidendritic cell monoclonal antibody, but not with an antimacrophage antibody. Interdigitating dendritic cells were identified in the granulomatous lymph node by staining with the antidendritic cell antibody and by transmission electron microscopy. When cultured in vitro with purified T lymphocytes, these cells acted as accessory cells for both purified protein derivative and concanavalin A induced proliferation. Although previous studies have shown that macrophages from these lymph nodes do not act as accessory cells, the present results indicate that dendritic cells from M. leprae granuloma containing lymph nodes may act as antigen-presenting cells.

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Section: Mitogenmentioning

confidence: 99%

Accessory Cell Function of Dendritic Cells from Lymph Nodes containing <i>Mycobacterium leprae</i> Induced Granulomas

Verghese

Healey

Curtis

et al. 1988

Int Arch Allergy Immunol

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“…T Lymphocytes were enriched by separation of whole lymph node cells on the basis of T cell adherence to nylon wool as previ ously described by Healey et al [1987b],…”

Section: T Lymphocytesmentioning

confidence: 99%

“…This system was used to quantitate the expres sion of the three monoclonal antibodies on B cells (i.e., those cells expressing surface immunoglobulin). A detailed methodology has been published previously [Healey et al, 1987b]. …”

Section: Double Immunofluorescence O F Lymph Node Cellsmentioning

confidence: 99%

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Behaviour of Guinea Pig T Cells Stimulated by Antigen, Allo-Antigen, and Mitogen

Healey

Agha

Turk

1988

Int Arch Allergy Immunol

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The expression of major histocompatibility (MHC) antigens on guinea pig T cells was used as a functional marker for lymphocyte activation. Antigen-stimulated lymphocytes were recovered from guinea pigs responding to the contact sensitizer DNFB, and isolated T cells were then phenotyped using a new anti-guinea pig monoclonal antibody, MSgp7. The level of expression of MHC class II, as defined by the monoclonal antibody, MSgp8, was increased on T cells recovered 4 days after sensitization, as compared with unsensitized controls. The value of this experiment was extended by measuring MHC class II expression on T cells stimulated in vitro by the mitogen concanavalin A, where a clear increase in MSgp8 binding was also observed. Confirmation of the specificity of MSgp8 for guinea pig MHC class II antigens was achieved by studying the inhibitory capacity of this antibody on an MHC class II restricted mixed leucocyte reaction. The combination of antibodies MSgp7 and MSgp8 with flow cytometry could be applied to other guinea pig experimental models to quantitate the expression of MHC class II antigens on T cells to determine their putative value in disease manifestation.

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“…Our results showed that the increased proportion of small lymphocytes was limited to Vac animals only. In this regard, Healey, Parker and Turk (1987) have described two distinct populations of SLN T cells in guineapigs based on FSC of flow cytometry. Activated T cells that mediate contact sensitivity in the DNFB (2,4-dinitrofluorobenzene) model are of low FSC, i.e.…”

Section: Discussionmentioning

confidence: 99%

Accelerated influx of dendritic cells into the lymph nodes draining skin sites exposed to attenuated cercariae of Schistosoma mansoni in guinea‐pigs

Sato

Kamiya

1998

Parasite Immunology

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We compared temporal changes in the cell composition of the skin-draining lymph nodes (SLN) in guinea-pigs exposed percutaneously to normal or attenuated cercariae of Schistosoma mansoni. Different populations were analysed by flow cytometry of double-stained cells by monoclonal antibodies to the major histocompatibility complex class II molecule and lymph node cells of guinea-pigs. Exposure to S. mansoni caused a marked increase in the proportion of B cells and dendritic cells (DC) on day 2, reaching a peak number on day 4. These changes were comparable in both infected and vaccinated animals exposed to normal or attenuated parasites, respectively. Total number of DC, however, showed different kinetics; in infected animals, the number peaked on day 2 and then gradually declined, whereas it reached a higher peak on day 4 in vaccinated animal. Daily injection of bromo-deoxyuridine after exposure to the parasite reduced the total number of DC in the SLN on day 4. A reduction in DC counts in the contralateral side SLN was also evident in vaccinated animals. Our results indicate that a significant number of newly formed DC are recruited to the skin by 4th day of vaccination, followed by increased efflux to the SLN. It is possible that retention of attenuated S. mansoni in the skin may cause accelerated recruitment of newly formed DC from the bone marrow, and facilitate transport and processing of antigens highly expressed on attenuated parasites.

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Distribution of a Guinea Pig Lymphocyte Cell Surface Antigen of Defined Function, as Detected by the Mouse Monoclonal Antibody MSgp 1

Cited by 4 publications

References 13 publications

Accessory Cell Function of Dendritic Cells from Lymph Nodes containing <i>Mycobacterium leprae</i> Induced Granulomas

Accessory Cell Function of Dendritic Cells from Lymph Nodes containing <i>Mycobacterium leprae</i> Induced Granulomas

Behaviour of Guinea Pig T Cells Stimulated by Antigen, Allo-Antigen, and Mitogen

Accelerated influx of dendritic cells into the lymph nodes draining skin sites exposed to attenuated cercariae of Schistosoma mansoni in guinea‐pigs

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