Distribution of antibiotic resistance genes in glacier environments

Segawa, Tomio; Takeuchi, Nozomu; Rivera, Andrés; Yamada, Akinori; Yoshimura, Yoshitaka; Barcaza, Gonzalo; Shinbori, Kunio; Motoyama, Hideaki; Kohshima, Shiro; Ushida, Kazunari

doi:10.1111/1758-2229.12011

Cited by 180 publications

(119 citation statements)

References 46 publications

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“…UPL probes have been used successfully in a variety of qPCR studies, including the quantification of bacterial antibiotic resistance genes in environmental samples (42,43). Using UPL probes, we can easily design TaqMan qPCR assays that can be run in identical PCR conditions, which is essential when applying qPCR assays to microfluidic qPCR.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Quantification of Multiple Food- and Waterborne Pathogens by Use of Microfluidic Quantitative PCR

Ishii

Segawa

Okabe

2013

Appl Environ Microbiol

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127

159

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bThe direct quantification of multiple pathogens has been desired for diagnostic and public health purposes for a long time. In this study, we applied microfluidic quantitative PCR (qPCR) technology to the simultaneous detection and quantification of multiple food-and waterborne pathogens. In this system, multiple singleplex qPCR assays were run under identical detection conditions in nanoliter-volume chambers that are present in high densities on a chip. First, we developed 18 TaqMan qPCR assays that could be run in the same PCR conditions by using prevalidated TaqMan probes. Specific and sensitive quantification was achieved by using these qPCR assays. With the addition of two previously validated TaqMan qPCR assays, we used 20 qPCR assays targeting 10 enteric pathogens, a fecal indicator bacterium (general Escherichia coli), and a process control strain in the microfluidic qPCR system. We preamplified the template DNA to increase the sensitivity of the qPCR assays. Our results suggested that preamplification was effective for quantifying small amounts of the template DNA without any major impact on the sensitivity, efficiency, and quantitative performance of qPCR. This microfluidic qPCR system allowed us to detect and quantify multiple pathogens from fecal samples and environmental water samples spiked with pathogens at levels as low as 100 cells/liter. These results suggest that the routine monitoring of multiple pathogens in food and water samples is now technically feasible. This method may provide more reliable information for risk assessment than the current fecal contamination indicator approach.

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Section: Discussionmentioning

confidence: 99%

Simultaneous Quantification of Multiple Food- and Waterborne Pathogens by Use of Microfluidic Quantitative PCR

Ishii

Segawa

Okabe

2013

Appl Environ Microbiol

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127

159

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“…Antibiotic resistance genes are often located on plasmids that are able to be horizontally transferred into diverse bacterial popula− tions contributing to the widespread dissemination of antibiotic resistance in the en− vironment (Davison 1999;Michaud et al 2004). Antibiotic−resistant bacteria have also detected in cold environments such as sandstones in McMurdo valley, Antarc− tica (Siebert et al 1996); the Arctic permafrost subsoil in Siberia (Mindlin et al 2008); near Palmer Station, Antarctica (Miller et al 2009); West Antarctica King George Island (Wong et al 2011); glacier environments in the Arctic, Antarctic, Central Asia, North and South America (Segawa et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Tomova¹,

Stoilova−Disheva²,

Vasileva−Tonkova³

2014

Polish Polar Research

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Abstract:In this study, selected heavy metals resistant heterotrophic bacteria isolated from soil samples at the Windmill Islands region, Wilkes Land (East Antarctica), were character− ized. Phylogenetic analysis revealed affiliation of isolates to genera Bacillus, Lysini− bacillus, Micrococcus and Stenotrophomonas. The strains were found to be psychrotolerant and halotolerant, able to tolerate up to 10% NaCl in the growth medium. The Minimum In− hibitory Concentration of the seven heavy metals Cr, Cu, Ni, Co, Cd, Zn, and Pb was deter− mined in solid media for each bacterial strain. Gram−positive Vi−2 strain and Gram−negative Vi−4 strain showed highest multiply heavy metals resistance, and Vi−3 and Vi−4 strains showed multi−antibiotic resistance to more than a half of the 13 used antibiotics. Plasmids were detected only in Gram−negative Vi−4 strain. The bacteria were able to produce differ− ent hydrolytic enzymes including industrially important proteases, xylanases, cellulases, and b−glucosidases. High heavy metals resistance of the Antarctic bacteria suggests their potential application for wastewater treatment in cold and temperate climates. Highly sensi− tive to Cd and Co ions Vi−1, Vi−5 and Vi−7 strains would be promising for developing biosensors to detect these most toxic heavy metals in environmental samples.

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“…In freshwater, a strong relationship between the source of pollution and the quantitative and spatial distribution of ARGs has been shown (Czekalski et al, 2014). Moreover many reports hint to a non-negligible amount of ARGs even in areas that are not directly impacted, such as permafrost and caves (D'Costa et al, 2011;Bhullar et al, 2012;Segawa et al, 2013).…”

Section: Antibiotic Resistance: Mechanisms Spread and Relations Betwmentioning

confidence: 99%

Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

2016

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Distribution of antibiotic resistance genes in glacier environments

Cited by 180 publications

References 46 publications

Simultaneous Quantification of Multiple Food- and Waterborne Pathogens by Use of Microfluidic Quantitative PCR

Simultaneous Quantification of Multiple Food- and Waterborne Pathogens by Use of Microfluidic Quantitative PCR

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

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