Distribution of biotypes and antimicrobial susceptibility of <i>Actinobacillus actinomycetemcomitans</i>

Ávila-Campos, Mário Júlio; Carvalho, Maria Auxiliadora Roque de; Zelante, F

doi:10.1111/j.1399-302x.1995.tb00171.x

Cited by 13 publications

(17 citation statements)

References 15 publications

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“…In this study, by culture, A. actinomycetemcomitans was isolated from 18% of the periodontal patients and 2% of the healthy subjects, in accordance with Asikainen et al (1) and Malheiros and AvilaCampos (22). Moreover, studies have shown that the occurrence of A. actinomycetemcomitans varied from 20% to 80% in adult periodontitis patients (3,22,33). On the other hand, by using a PCR method, this microorganism was detected in 44% of the periodontal patients, and in 16% of the healthy subjects.…”

Section: Discussionsupporting

confidence: 87%

“…In healthy subjects, biotypes II, VI and X were observed. Few studies have shown the presence of biotypes in A. actinomycetemcomitans and the biotype II appears to be the most prevalent in Brazilian periodontal patients (3,22).…”

Section: Discussionmentioning

confidence: 99%

“…This microorganism is grouped into 10 different biotypes, and their distribution depends on several factors, such as, geographic location and clinical condition of the patients (3,28). However, there is a need to confirm this statement in Brazilian population, which presents several peculiarities, such as racial miscegenation and cultural aspects.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Gaetti‐Jardim¹,

Wahasugui²,

Tomazinho³

et al. 2008

Braz. J. Microbiol.

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Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18%) of the periodontal patients and one (2%) healthy subject. However, by PCR, this organism was detected in 44% of the periodontal patients and in 16% of the healthy subjects. It was verified a great discrepancy between PCR detection of the ltx operon promoter directly from crude subgingival biofilm and from bacterial DNA. Only one periodontal sample harbored highly leukotoxic A. actinomycetemcomitans. Moreover, biotype II was the most prevalent and no correlation between biotypes and leukotoxic activity was observed. The diversity of leukotoxin expression by A. actinomycetemcomitans suggests a role of this toxin in the pathogenesis of periodontal disease and other infectious diseases.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Gaetti‐Jardim¹,

Wahasugui²,

Tomazinho³

et al. 2008

Braz. J. Microbiol.

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“…13,8 Para profundizar en el estudio de A. actinomycetemcomitans se puede biotipar haciendo pruebas de fermentación de carbohidratos, dextrina, maltosa, manitol y xilosa. 23,25 Las pruebas serológicas se realizan para identificar los 7 serotipos (A -G). 26,28 Pruebas moleculares como PCR convencional y en tiempo real, son usados por ser muy sensibles y específicos para el diagnóstico de microorganismos.…”

Section: Fisiopatologíaunclassified

“…6 Es de importancia mencionar la transmisión del A. actinomycetemcomitans de padres a hijos, ya que estudios de biotipos y serotipos bacterianos en los componentes de la familia, han demostrado un contagio de tipo intrafamiliar. 25,28,31 Tratamiento mecánico y antimicrobiano para A. actinomycetemcomitans El tratamiento mecánico se basa en la fase I de la terapia periodontal, la cual consiste en la eliminación de placa y tártaro supra y subgingival, así como un alisado de la superficie radicular, para evitar superficies rugosas que permitan mayor retención de placa. Instruir al paciente en el control de placa por debajo del 20 % del índi-ce de higiene según O'leary y de una reevaluación periódica de su caso.…”

Section: A Actinomycetemcomitans Pertenece Al Grupo Hacek (H Aphropunclassified

Aggregatibacter actinomycetemcomitans: Patógeno importante en la periodontitis

Perfecto

Nakata

Cadillo

et al. 2014

Odontol. sanmarquina

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Aggregatibacter actinomycetemcomitans es un patógeno muy estudiado en los cuadros clínicos de periodontitis, siendo ya identificada a principio del siglo XX; con los años ha sufrido cambios en su denominación y se han descubierto múltiples factores de virulencia que lo hacen un patógeno importante en la enfermedad periodontal, específicamente en la periodontitis agresiva localizada. Esta revisión tratará de explicar su morfología, factores de virulencia, cultivo y demás características de importancia.

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Endarteritis and mycotic aortic aneurysm caused by an oral strain ofActinobacillus actinomycetemcomitans

Martín¹,

Andrés²,

Fierro³

et al. 1998

Eur. J. Clin. Microbiol. Infect. Dis.

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Actinobacillus actinomycetemcomitans was isolated from blood cultures of a 33-year-old febrile patient with a previously undiagnosed coarctation of the aorta. Subgingival samples from diseased periodontal pockets revealed the presence of A. actinomycetemcomitans. An infected (mycotic) aortic aneurysm and endarteritis were diagnosed and surgically treated. The identity of blood and oral clinical isolates of A. actinomycetemcomitans was supported by genetic analysis, including fingerprinting by restriction fragment length polymorphism, ribotyping, and random amplified polymorphic DNA; biotyping; and antibiogram typing. These data strongly suggest that the periodontal pockets were the primary source of A. actinomycetemcomitans endarteritis in this case.

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Distribution of biotypes and antimicrobial susceptibility of Actinobacillus actinomycetemcomitans

Cited by 13 publications

References 15 publications

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis

Aggregatibacter actinomycetemcomitans: Patógeno importante en la periodontitis

Endarteritis and mycotic aortic aneurysm caused by an oral strain ofActinobacillus actinomycetemcomitans

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