Distribution of blood-group-related carbohydrate antigens on oral endothelial cells

Abstract

Recent studies indicate that carbohydrate structures are involved in various endothelial functions such as inflammatory processes, adhesion of metastatic cells to endothelium and endothelial differentiation. In this paper we report the endothelial expression of various blood-group-related carbohydrate structures in normal oral mucosa using 15 different monoclonal antibodies reacting with type 1, 2 or 3 carbohydrate chains. Twenty biopsies, including normal oral mucosa, from secretor individuals comprised nine blood group O, nine A, one B and one AB. Endothelial staining was compared with epithelial staining in the same biopsies. Five blood-group-related carbohydrate antigens were detected on endothelial cells. The H type 2 antigen, which is the precursor of A, B and AB antigens, has previously been believed to be a universal marker for endothelial cells. All blood group O individuals (n = 9) showed strong H antigen staining in the endothelium of most vessels. However, of blood group A, B and AB individuals (n = 11), four showed heterogenous H antigen staining. In addition, we found that six out of ten blood group A or AB individuals, who expressed A or A and B antigens on spinous squamous cells and glandular epithelium, showed either heterogenous or no staining for these structures on their endothelial cells. It is concluded that there are differences between the biosynthesis of blood-group-related carbohydrate antigens in oral endothelium and epithelium.

Human Umbilical Vein Endothelial Cells Differ from Other Endothelial Cells in Failing to Express ABO Blood Group Antigens

“…Most adult endothelial cells have been shown to express appropriate AB antigens in vivo [24]. Oral endothelium is the only other endothelial cell which has been described as lacking blood-group-related carbohydrate antigens [25], although weak AB expression has also been demonstrated for intra-mammary lymphatic endothelium in breast carcinomas [26]. …”

Molecular Deciphering of the ABO System as a Basis for Novel Diagnostics and Therapeutics in ABO Incompatible Transplantation

“…Most of the information on EC expression of ABH antigens is derived from immunohistochemical studies [37,[90][91][92], and very few investigations on the chemical structure of EC ABH determinants have been performed. However, proteomics and glycomics techniques are likely to change that in a near future [93].…”

“…Vascular EC, the barrier between the host's immune system and the grafted organ, mainly carry ABH determinants on the type 2 core chain [90]. Again, this knowledge was based on immunohistochemical studies [90] and isolation of glycosphingolipidbased ABH antigens [36,111,112].…”

“…Again, this knowledge was based on immunohistochemical studies [90] and isolation of glycosphingolipidbased ABH antigens [36,111,112]. Recently, vascular EC at sites of inflammation were shown to aberrantly express A type 3 determinants [113].…”

Inhibition of adherence of corynebacterium diphtheriae to human buccal epitelium by glycoside hydrolases of marine hydrobiontes

“…На клетках буккального эпителия идентифицированы различные варианты АВН изотипов углеводных цепей, соответствующие АВН антигенам групп крови эритроцитов индивидуума [26]. Принимая во внимание способность АВН антигенов участвовать в различных межклеточных взаимодействиях и взаимодействиях клеток с матриксом, результаты, демонстрирующие высокую антиадгезивную активность a-галактозидазы в нашем случае (использование эпителиоцитов донора с B (III) группой крови), могут быть обусловлены, в числе прочих возможных факторов, специфичностью фермента, обеспечивающей нарушение углеводных структур, входящих в состав В-антигенов.…”