Distribution of Corticotropin-Releasing Factor and Urocortin 1 in the Vole Brain

Lim, Miranda M.; Tsivkovskaia, Natalia O.; Bai, Yaohui; Young, Larry J.; Ryabinin, Andrey E.

doi:10.1159/000094360

Cited by 40 publications

(50 citation statements)

References 83 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with this hypothesis are data from rats showing a more pronounced habituation of corticosterone responses to chronic restraint stress in males versus females (Galea et al, 1997). Sex differences in the central synthesis or effects of other neuropeptides, including vasopressin (DeVries & Simerly, 2002;DeVries & Panzica, 2006) or CRF (Lim et al, 2005;Lim et al, 2006), may also mediate the neuroendocrine responses described here.…”

Section: Discussionsupporting

confidence: 84%

Social isolation induces behavioral and neuroendocrine disturbances relevant to depression in female and male prairie voles

Grippo

Gerena

Huang

et al. 2007

Psychoneuroendocrinology

302

279

View full text Add to dashboard Cite

SummarySupportive social interactions may be protective against stressors and certain mental and physical illness, while social isolation may be a powerful stressor. Prairie voles are socially monogamous rodents that model some of the behavioral and physiological traits displayed by humans, including sensitivity to social isolation. Neuroendocrine and behavioral parameters, selected for their relevance to stress and depression, were measured in adult female and male prairie voles following 4 weeks of social isolation versus paired housing. In Experiment 1, oxytocin-immunoreactive cell density was higher in the hypothalamic paraventricular nucleus (PVN) and plasma oxytocin was elevated in isolated females, but not males. In Experiment 2, sucrose intake, used as an operational definition of hedonia, was reduced in both sexes following 4 weeks of isolation. Animals then received a residentintruder test, and were sacrificed either 10 minutes later for the analysis of circulating hormones and peptides, or two hours later to examine neural activation, indexed by c-Fos expression in PVN cells immunoreactive for oxytocin or corticotropin-releasing factor (CRF). Compared to paired animals, plasma oxytocin, ACTH and corticosterone were elevated in isolated females and plasma oxytocin was elevated in isolated males, following the resident-intruder test. The proportion of cells doublelabeled for c-Fos and oxytocin or c-Fos and CRF was elevated in isolated females, and the proportion of cells double-labeled for c-Fos and oxytocin was elevated in isolated males following this test. These findings suggest that social isolation induces behavioral and neuroendocrine responses relevant to depression in male and female prairie voles, although neuroendocrine responses in females may be especially sensitive to isolation.

show abstract

Section: Discussionsupporting

confidence: 84%

Social isolation induces behavioral and neuroendocrine disturbances relevant to depression in female and male prairie voles

Grippo

Gerena

Huang

et al. 2007

Psychoneuroendocrinology

302

279

View full text Add to dashboard Cite

show abstract

“…Although CRF has been shown to bind preferentially to CRF 1 , it also binds to CRF 2 with substantial affinity (Primus et al, 1997). Ucn-1-immunoreactive fibers were not seen in NAcc, but were observed in other regions of the brain such as the Edinger-Westphal nucleus (Lim et al, 2006). We were unable to map Urocortin-2 or Urocortin-3 fibers in the vole brain due to lack of specific immunostaining; however, it would be interesting to determine if these potential ligands, which also bind CRF 2 receptors with high affinity, are also present in NAcc along with CRF 2 receptors.…”

Section: Discussionmentioning

confidence: 91%

“…Dissected brains were postfixed overnight in the 2% paraformaldehyde/PBS solution and cryoprotected in 30% sucrose/PBS. Thirty μm thick freefloating coronal sections were sliced on a cryostat and processed for immunohistochemistry according standard protocols (Ryabinin, Criado, Henriksen, Bloom, and Wilson, 1997;Weitemier, Tsivkovskaia, and Ryabinin, 2005) with adjustments for vole tissue made in earlier experiments (Lim et al, 2006). Briefly, endogenous peroxidase activity was quenched by 15-minute incubation with 0.3% hydrogen peroxide.…”

Section: Crf and Urocortin-1 Immunohistochemistrymentioning

confidence: 99%

“…We have previously shown that, although the distributions of CRF mRNA and peptide appear highly conserved among vole species, the distributions of CRF receptor types 1 and 2 (CRF 1 and CRF 2 ) dramatically differ throughout the brain in four vole species exhibiting varying social organizations (Lim, Nair, and Young, 2005;Lim, Tsivkovskaia, Bai, Young, and Ryabinin, 2006). Receptor binding appeared to correlate with monogamous social structure in several brain regions; however, only the nucleus accumbens (NAcc) consistently segregated with both monogamous vole species and both nonmonogamous vole species.…”

mentioning

confidence: 98%

See 1 more Smart Citation

CRF receptors in the nucleus accumbens modulate partner preference in prairie voles

Lim

Liu

Ryabinin

et al. 2007

Hormones and Behavior

Self Cite

102

View full text Add to dashboard Cite

Recent evidence suggests a role for corticotropin-releasing factor (CRF) in the regulation of pair bonding in prairie voles. We have previously shown that monogamous and non-monogamous vole species have dramatically different distributions of CRF receptor type 1 (CRF 1 ) and CRF receptor type 2 (CRF 2 ) in the brain, and that CRF 1 and CRF 2 receptor densities in the nucleus accumbens (NAcc) are correlated with social organization. Monogamous prairie and pine voles have significantly lower levels of CRF receptor type 1 (CRF 1 ), and significantly higher levels of type 2 (CRF 2 ) binding, in NAcc than non-monogamous meadow and montane voles. Here, we report that microinjections of CRF directly into the NAcc accelerate partner preference formation in male prairie voles. Control injections of CSF into NAcc, and CRF into caudate-putamen, did not facilitate partner preference. Likewise, CRF injections into NAcc of non-monogamous meadow voles also did not facilitate partner preference. In prairie voles, this CRF-facilitation effect was blocked by co-injection of either CRF 1 or CRF 2 receptor antagonists into NAcc. Immunocytochemical staining for CRF and Urocortin-1 (Ucn-1), two endogenous ligands for CRF 1 or CRF 2 receptors in the brain, revealed that CRF, but not Ucn-1, immunoreactive fibers were present in NAcc. This supports the hypothesis that local CRF release into NAcc could activate CRF 1 or CRF 2 receptors in the region. Taken together, our results reveal a novel role for accumbal CRF systems in social behavior.Keywords nucleus accumbens; attachment; CRF1; CRF2; corticotropin-releasing factor; corticotrophinreleasing hormone; vole; neuropeptide receptors; pair bond; social behavior; monogamy; species differencesThe corticotropin-releasing factor (CRF) system is involved in the neurobiology underlying stress and anxiety, but much less is known about its role in social behavior. Microtine rodents exhibit diverse social organizations and thus offer an excellent comparative approach in the study of the neurobiology of social behavior (Young and Wang, 2004 Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. (Getz, Carter, and Gavish, 1981;Salo, Shapiro, and Dewsbury, 1993). In contrast, closely-related meadow (Microtus pennsylvanicus) and montane voles (Microtus montanus) are promiscuous and solitary (Gruder-Adams and Getz, 1985;Shapiro and Dewsbury, 1990). Past research has revealed that the brain distribution of neuropeptide receptors for oxytocin and vasopressin appears to be responsible for the species differences in social organization (Insel and Shapiro, 1992;In...

show abstract

“…Immunohistochemistry, fluorescent in situ hybridization, and TUNEL staining Four adjacent 4 lm myocardial sections were serially cut from the same wax blocks of each group, and stained with either an anti-desmin antibody (used as a marker of cardiac cells), or an antisense urocortin cRNA probe synthesized with a 3 0 -biotinylated tail, or an anti-urocortin antibody, or a fluorescein-conjugated TUNEL labeling, with propidium iodide used as a counterstaining [9,13,14]. The urocortin probe consisted of 48 bases (AAA-TAA-CCA-CTC-AGA-GTA-TTC-AGG-GTG-ACT-CTG-GGC-GCG-CTG-CAC-CGC) and was complementary to the rat mRNA encoding base pairs 571-618 of the urocortin message (GenBank accession number NM021290).…”

Section: 2mentioning

confidence: 99%

Retracted: Cardiac release of urocortin precedes the occurrence of irreversible myocardial damage in the rat heart exposed to ischemia/reperfusion injury

Knight¹,

Chen‐Scarabelli²,

Yuan³

et al. 2008

FEBS Letters

View full text Add to dashboard Cite

This study evaluates whether cardiac ischemia induces release of urocortin, before and independently from myocyte cell death. Urocortin levels rose after 5-min ischemia and peaked after 10-min ischemia, when cell death was not detected. However, myocyte apoptosis and/or necrosis occurred following 20-and 30-min ischemia, which paralleled a fall in urocortin levels, suggesting that urocortin expression and release are mainly sustained by metabolically challenged, though still viable myocytes. Hence, since cardiac release of urocortin, unlike that of conventional biomarkers, occurs before and apart from cell death, urocortin levels may be clinically useful in the diagnosis of sublethal myocardial ischemia.

show abstract

Distribution of Corticotropin-Releasing Factor and Urocortin 1 in the Vole Brain

Cited by 40 publications

References 83 publications

Social isolation induces behavioral and neuroendocrine disturbances relevant to depression in female and male prairie voles

Social isolation induces behavioral and neuroendocrine disturbances relevant to depression in female and male prairie voles

CRF receptors in the nucleus accumbens modulate partner preference in prairie voles

Retracted: Cardiac release of urocortin precedes the occurrence of irreversible myocardial damage in the rat heart exposed to ischemia/reperfusion injury

Contact Info

Product

Resources

About