Distribution of endotoxin-51-Cr in normal and endotoxin-resistant dogs

Starzecki, B; Reddin, JL; Gran, Arthur; Ww, Spink

doi:10.1152/ajplegacy.1967.213.5.1065

Cited by 7 publications

(2 citation statements)

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“…Endotoxin is known to be phagocytically removed from the circulation primarily by hepatic macrophages (22). In addition, the endotoxin detoxifying activity of liver displays both a latency of activation, i.e., probably requires disruption of lysosomes to release lytic enzymes in vitro, (21) and an acid pH optimum (23).…”

mentioning

confidence: 99%

Degradation of Particulate Lipid by the Large-Granule Fraction of Rat Liver

Cornell

Saba

1975

Experimental Biology and Medicine

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confidence: 99%

Degradation of Particulate Lipid by the Large-Granule Fraction of Rat Liver

Cornell

Saba

1975

Experimental Biology and Medicine

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“…For polymyxin to be protective 30 min after endotoxin, endotoxemia would have to be present for this peri- od. While such evidence in the embryo is lacking, 51Cr tagged endotoxin remains in the plasma of dogs for considerably longer periods of time (9). Presumably sufficient active endotoxin remains in the embryo circulation for at least 30 min, or at least remains accessible to binding, which blocks endotoxin effect.…”

mentioning

confidence: 99%

Temporal Protection of Chick Embryo against Endotoxin by Isoproterenol, Polymyxin B Sulfate, and Hydrocortisone

Gruninger

Howe

1969

Experimental Biology and Medicine

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The lethal effect of endotoxin in the 10-11 day chick embryo could be prevented by the administration of hydrocortisone ( 1 ) , isoproterenol (2), or polymyxin B sulfate (3) when endotoxin and the protectorant were given simultaneously. Since the properties of these agents differ, a variance in protection could be anticipated with a change in the time between the administration of endotoxin and protectorant. To preclude a nonspecific effect of in vitro mixing of endotoxin and a protectorant, separate administration vas desirable. This is a further report on lethality studies in the chick embryo when the three aforementioned agents were administered at varying time intervals before and after the administration of LD50-100 dose of endotoxin.Methods. White leghorn fertile eggs (Ghostley Pearl, Anoka, Minnesota) were used throughout. Ten-day eggs were candled and a large vein was exposed by removal of the overlying shell. After a 60-min interval which enhanced the exposed vessel's resistance to the trauma of injection, a drop of sterile mineral oil was placed on the membrane for better visualization of the vessel when transilluminated. The same vessel or a similarly prepared one was used for the second intravenous injection. A total of 1.5 minims was injected each time. Eggs with traumatic bleeding were discarded. The eggs were placed in a humidified incubator set at 99.5OF between the first and second injection and were returned to the incubator after the second injection for 18-24 hr, at which time the viability of embryos was determined by candling. All nonviable embryos and those with questionable viability were examined grossly.Only those embryos with clear evidence of intra-embryo hemorrhage were recorded as experimental deaths.Materials. Endotoxin: a single lot of Esch erichia coli endotoxin prepared in our laboratory as previously described ( 5 ) was used in all experiments. Pro tec torants : sterile in jectable isoproterenol hydrochloride (Isuprel) ,1 polymyxin B sulfate,2 and hydrocortisone sodium succinate (Solu-Cortef ) were used throughout. Each 10 pg of Isuprel contained 6 pg of lactic acid, 90 pg of sodium lactate, 350 pg of sodium chloride and 50 pg of sodium bisulfite. Each 25 pg of hydrocortisone sodium succinate contained 0.2 pg of anhydrous sodium biphosphate, 0.62 pg of methyl paraben, 0.02 pg of propyl paraben and 2.2 pg of exsiccated sodium phosphate. These additives with the respective drugs were not independently controlled. Incubator : a single stage humidity and temperature controlled incubator was used to house the developing and treated embryos? Syringes: disposable sterile, nontoxic, nonpyrogenic, mono jet 501-M 1 cc syringes graduated in minims with a 27-gauge needle were used for all in jections.6Results. A total of 25 experiments with appropriate saline controls were carried out. Each experiment compared endotoxin with one or more protectorants used independently and with saline control at one or more time intervals. The results in the Tables represent the sum total of embryos in each...

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Exogenous Pyrogens

Dinarello¹,

Wolff²

1982

Pyretics and Antipyretics

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Distribution of endotoxin-51-Cr in normal and endotoxin-resistant dogs

Cited by 7 publications

References 0 publications

Degradation of Particulate Lipid by the Large-Granule Fraction of Rat Liver

Degradation of Particulate Lipid by the Large-Granule Fraction of Rat Liver

Temporal Protection of Chick Embryo against Endotoxin by Isoproterenol, Polymyxin B Sulfate, and Hydrocortisone

Exogenous Pyrogens

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