Distribution of fim3 and flaA TTGE sequence types amongst isolates of Bordetella bronchiseptica from different host animals

Shina, A.; Hart, C. A.; Stenton, Matthew D.; Dawson, Susan; McCracken, Christina; Binns, S. H.; Gaskell, Rosalind M.; Winstanley, Craig

doi:10.1099/0022-1317-51-7-557

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…Results obtained in prior studies using a variety of methods suggest a considerable degree of genetic diversity exists among B. bronchiseptica isolates, including those obtained from a single host or from the same geographical region (Khattak & Matthews, 1993;Gueirard et al, 1995; Binns et al, 1998;Keil & Fenwick, 1999;Register & Magyar, 1999;Shina et al, 2002). In contrast, ribotyping, MLST and pertactin repeat region sequence analysis reported here confirm and extend the findings of a prior investigation suggesting a highly clonal population of B. bronchiseptica is found in seals of the North Sea (Register et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates

Ivanov²,

Harvill³

et al. 2015

Microbiology

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During a succession of phocine morbillivirus outbreaks spanning the past 25 years, Bordetella bronchiseptica was identified as a frequent secondary invader and cause of death. The goal of this study was to evaluate genetic diversity and the molecular basis for host specificity among seal isolates from these outbreaks. MLST and PvuII ribotyping of 54 isolates from Scottish, English or Danish coasts of the Atlantic or North Sea revealed a single, host-restricted genotype. A single, novel genotype, unique from that of the Atlantic and North Sea isolates, was found in isolates from an outbreak in the Caspian Sea. Phylogenetic analysis based either on MLST sequence, ribotype patterns or genome-wide SNPs consistently placed both seal-specific genotypes within the same major clade but indicates a distinct evolutionary history for each. An additional isolate from the intestinal tract of a seal on the south-west coast of England has a genotype otherwise found in rabbit, guinea pig and pig isolates. To investigate the molecular basis for host specificity, DNA and predicted protein sequences of virulence genes that mediate host interactions were used in comparisons between a North Sea isolate, a Caspian Sea isolate and each of their closest relatives as inferred from genome-wide SNP analysis. Despite their phylogenetic divergence, fewer nucleotide and amino acid substitutions were found in comparisons of the two seal isolates than in comparisons with closely related strains. These data indicate isolates of B. bronchiseptica associated with respiratory disease in seals comprise unique, host-adapted and highly clonal populations.

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Section: Discussionmentioning

confidence: 99%

Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates

Ivanov²,

Harvill³

et al. 2015

Microbiology

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“…By using TGGE method, we need standard specimen of each species of Borrelia to be electrophoresed in the same gel, in order to identify a species of unknown origin by a comparison of the relative position of the DNA bands. The same principle of species differentiation by TGGE was used in several studies: Monstein et al [16] identified enterococcal isolates and profiling bacterial flora from gastric biopsies [16]; Vasquez et al [31] made identification of Lactobacillus species; and Shina et al [19] performed typing of B. bronhiseptica isolates. We believe that the TGGE method, in comparison with PFGE and restriction of PCR product, has advantages.…”

Section: Discussionmentioning

confidence: 99%

“…Among others, TGGE has proven to be a useful tool in genetic analyses of various pathogenic species of bacteria or bacterial strains differentiation [16][17][18][19]. TGGE method enables differentiation of duplex DNA fragments of the same length, but different nucleotide sequence [20].…”

Section: Introductionmentioning

confidence: 99%

Differentiation ofBorrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis

2006

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Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely PFGE and restriction of 5S-23S intergenic space region PCR product. A differentiation of B. garinii, B. afzelii, and B. burgdorferi senso stricto species with TGGE was possible and intraspecies variation was detected. Results compared between TGGE, PFGE, and restriction of 5S-23S intergenic space region PCR product showed no difference in specificity of species identification.

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“…haemagglutinin (fha), fimbriae (fim2, fim3, fimX and fimA) and pertactin (prn), and exotoxins as bifunctional adenylate cyclase haemolysin (achly), dermonecrotic toxin (dnt) and tracheal colonization factor (tcfA) (Cotter and Miller, 2001;Shina et. al.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Bordetellosis in Pigs from North East India by PCR

Mazumder¹,

Kar²,

Shome³

et al. 2012

MJM

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Bordetella bronchiseptica is an etiologic agent of bronchopneumonia and progressive atrophic rhinitis (PAR) in swine. Both toxigenic and nontoxigenic B. bronchiseptica strains have been associated with bronchopneumonia. Monitoring and investigation of outbreaks involving these bacteria require sensitive and accurate identification and reliable determination of the isolates. In the present study, we report the development, optimization and performance characteristics of polymerase chain reaction (PCR) for B. bronchiseptica strains. A total of 47 isolates of B. bronchiseptica were biochemically identified from 90 pigs suffering from bronchopneumonia maintained in a semi intensive rearing system of organized piggery in Meghalaya. PCR was employed with filamentous hemagglutinin toxin genes (fhaB and fhaC) and fimbrial toxin genes (fim2 and fim3) primers to identify the specific toxin types of B. bronchiseptica. All the 47 isolates were positive for all the toxin genes. The specifity of designed primer pairs was tested by screening some common bacterial species related to the respiratory tract namely, Pasteurella multocida, Staphylococcus aureus and Streptococcus spp. No DNA amplifications of the organisms tested could be seen in the specificity test. Amplicon mobility in agarose gels indicates the amplicons are highly stable. The data presented, establish this PCR as a reliable method for identification and study of adhesins of B. bronchiseptica that may greatly simplify investigations of swine bronchopneumonia and PAR for Indian isolates.

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Distribution of fim3 and flaA TTGE sequence types amongst isolates of Bordetella bronchiseptica from different host animals

Cited by 7 publications

References 28 publications

Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates

Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates

Differentiation ofBorrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis

Diagnosis of Bordetellosis in Pigs from North East India by PCR

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