Distribution of free N-acetylneuraminic acid in rat organs

Sugiyama, Naokazu; Saito, Kin-ichi; Itoh, Masayoshi; Miyata, Takeshi

doi:10.1016/0024-3205(89)90360-3

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…Although the method used for the determination of tissue SA levels in our study was different from those used in other studies [29,30,31], our findings were similar with theirs. In our study, the mean TSA levels of liver and heart tissues were between 394-633 µg per g wet tissue.…”

Section: Effect Of Hypercholesterolemiasupporting

confidence: 90%

Effect of Fenofibrate on Serum and Tissue Sialic Acid Levels in Short-term Experimental Hypercholesterolemia

Öztürk

Yarat

Emekli

2011

Arzneimittelforschung

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In recent years, sialic acid is considered to be a possible marker for cardiovascular diseases. The aim of this study was to investigate the effect of two different treatment periods of fenofibrate (CAS 49562-28-9) on serum, heart and liver sialic acid levels in experimental hypercholesterolemia. Serum, heart and liver total sialic acid levels were determined by Warren's thiobarbituric acid (TBA) method and serum lipid levels by commercial kits at the end of the fenofibrate treatment for 3 and 6 weeks. Fenofibrate treatment reduced serum total sialic acid levels significantly in the control and hypercholesterolemic groups at the end of the 3rd week and only in the control group at the end of the 6th week. Serum sialic acid levels of fenofibrate-treated hypercholesterolemic rats in the 6-week period were significantly higher than those in the 3-week period. Neither the hypercholesterolemic diet nor fenofibrate had any significant effect on heart and liver sialic acid levels. In conclusion, decreased serum sialic acid levels in control and hypercholesterolemic groups by fenofibrate short-term treatment may contribute to the decreased risk of cardiovascular diseases that accompanies the hypercholesterolemic complications. The decreased serum sialic acid levels further indicate the clinical efficacy of fenofibrate as an anti-inflammatory agent.

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Section: Effect Of Hypercholesterolemiasupporting

confidence: 90%

Effect of Fenofibrate on Serum and Tissue Sialic Acid Levels in Short-term Experimental Hypercholesterolemia

Öztürk

Yarat

Emekli

2011

Arzneimittelforschung

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“…Using a selected ion monitoring technique, we found that the sputa of bronchitic rabbits contained much higher levels of both free and bound NANA than the airway secretions of normal rabbits [62,63]. In addition, we found that NANA concentrations in the sputa of patients with chronic bronchitis (free: 15-204 mg·mL -1 ; bound: 276-1298 mg·mL -1 ) were also apparently higher than those in the bronchoalveolar lavages of healthy subjects.…”

Section: Sodium Aceneuramate (N-acetylneuraminic Acid (Nana) Sodium Smentioning

confidence: 92%

Current opinion of muco-active drug research: strategies and problems

Miyata

Kai²,

Isohama³

et al. 1998

Eur Respir J

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In general, mucoactive drugs are classified into several groups. However, since many drugs have overlapping effects, it is difficult to classify the drugs into groups based on their major actions. It has been reported that many mucoactive drugs have antioxidant effects. It is reasonable to suggest that an anti-inflammatory property is crucial to demonstrate effectiveness in a clinical context. From this point of view, we have evaluated several mucoactive drugs over two decades. Of these, we will consider the following drugs with anti-inflammatory properties: sodium aceneuramate; glucocorticoids; traditional Chinese medicines; and new cysteine derivatives. On the basis of these findings, we believe that the efforts to seek for compatible actions between glucocorticoids and oriental medicines may provide new opportunities for development of ideal mucoactive drugs with specified actions, i.e. suppression of gene expression.

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“…very selectivc and sensitive for NANA and suffer from interferences such as 2-deoxyglucose and 2-deoxyribose. Although the GUMS methods (Mononen and Karkkainen, 1975;Sugiyama et al, 1988Sugiyama et al, ,1989 are extremely sensitive, they require an expensive instrument and a rather tedious sample clean-up procedure. The spectorophotometric HPLC methods, based on the intact absorption of NANA (Silver et al, 1981;Shukla and Schauer, 1982) and the derivatization of NANA with 4,4'-dicarboxy-2,2'-biquinoline (Shukla et ul., 1982), 2-cyanoacetamide (Honda and Suzuki, 1984), TBA (Powcll and Hart, 1986) and benzoic anhydride (Karamanos et al.…”

Section: Introductionmentioning

confidence: 99%

“…The spectrophotometric (Warren, 1959;Aminoff, 1961) and spectrofluorometric (Hammond and Papermaster, 1976) methods using thiobarbituric acid (TBA) are not Author to whom correspondence should be addrcssed. very selectivc and sensitive for NANA and suffer from interferences such as 2-deoxyglucose and 2-deoxyribose.…”

Section: Introductionmentioning

confidence: 99%

“…Many methods, involving spectrophotometry (Warren, 1959;Aminoff, 1961), spectrofluorometry (Hammond and Papermaster, 1976), gas chromatography/mass spectrometry (GUMS) (Mononen and Karkkainen, 1975;Sugiyama et al, 1988Sugiyama et al, , 1989 and high performance liquid chromatography (HPLC) (Silver et al, 1981;Shukla and Schauer, 1982;Shukla et al, 1982;Honda and Suzuki, 1984;Kobayashi et al, 1985;Powell and Hart, 1986;Hara et al, 1986Hara et al, , 1987Karamanos et al, 1990), have been proposed for the determination of NANA in biological samples. The spectrophotometric (Warren, 1959;Aminoff, 1961) and spectrofluorometric (Hammond and Papermaster, 1976) methods using thiobarbituric acid (TBA) are not Author to whom correspondence should be addrcssed.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of N‐acetylneuraminic acid in human serum and urine by high performance liquid chromatography with chemiluminescence detection

Ishida

Nakahara

Yamaguchi

1992

Biomedical Chromatography

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A highly sensitive method for the determination of N-acetylneuraminic acid in human serum and urine is investigated. This method employs high performance liquid chromatography with chemiluminescence detection. N-Acetylneuraminic acid, released by hydrochloric acid hydrolysis of serum and urine, and N-glycolylneuraminic acid (internal standard) are converted into chemiluminescent derivatives with 4,5-diaminophthalhydrazide dihydrochloride, a chemiluminescence derivatization reagent for alpha-keto acids. The derivatives are separated within 35 min on a reversed phase column, TSKgel ODS-120T, with isocratic elution, followed by chemiluminescence detection; the chemiluminescence is produced by the reaction of the derivatives with hydrogen peroxide in the presence of potassium hexacyanoferrate(III) in alkaline solution. The detection limit for N-acetylneuraminic acid is 9 fmol (signal-to-noise ratio = 3). This sensitivity permits precise determination of N-acetylneuraminic acid in 10 nL of serum or 50 nL of urine. The method is applied to the determination of the N-acetylneuraminic acid in human sera from normal subjects and cancer patients and in normal urine.

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Distribution of free N-acetylneuraminic acid in rat organs

Cited by 5 publications

References 6 publications

Effect of Fenofibrate on Serum and Tissue Sialic Acid Levels in Short-term Experimental Hypercholesterolemia

Effect of Fenofibrate on Serum and Tissue Sialic Acid Levels in Short-term Experimental Hypercholesterolemia

Current opinion of muco-active drug research: strategies and problems

Measurement of N‐acetylneuraminic acid in human serum and urine by high performance liquid chromatography with chemiluminescence detection

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