Current opinion of muco-active drug research: strategies and problems

Miyata, Takeshi; Kai, Hirofumi; Isohama, Yoichiro; Takahama, Kazuo

doi:10.1183/09031936.98.11020480

Cited by 22 publications

(13 citation statements)

References 74 publications

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“…A direct effect of acetylcholine on tracheal secretory elements was further confirmed as a dose-dependent in studies by Kamijo et al (1993), Woods et al (1996), and Matera et al (1998). Miyata et al (1998) doubted the acetylcholine role in controlling production of high molecular weight-glycoconjugates in spite of the study by Steel and Hanrahan (1997) on GC of the tracheal epithelium in hamsters.…”

Section: Airways' Epithelium Cholinergic Stimulation Glycoconjugatementioning

confidence: 93%

“…Lamb and Reid (1972) and Damjanov (1987) held the decrease of acidic sialylated GCCs and the increase of acidic sulphated ones for a common reaction of the tracheal epithelium to general alteration. Miyata et al (1998) described the protective effects of sialic acids on the airways' epithelium. To complete our previous studies, we decided to study also the effect of i.v.…”

Section: Airways' Epithelium Cholinergic Stimulation Glycoconjugatementioning

confidence: 99%

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The Effect of Intravenously Administered acetylcholine on the Glycoconjugate Composition on Goblet Cells of the Tracheal Epithelium in Rabbits

Vajner¹,

Uhlı́k²,

Konrádová³

et al. 2000

Acta Vet. Brno

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To evaluate the influence of cholinergic stimulation on the composition of glycoconjugates in goblet cells, we studied the tracheal epithelium of rabbits 5 minutes and 20 minutes after i.v. administration of acetylcholine in doses 0.1 mg and 0.5 mg, respectively. Using both conventional and lectin histochemistry, we compared the percentage of tracheal goblet cells containing neutral glycoconjugates to those containing total acidic glycoconjugates, sulphated acidic glycoconjugates, and sialylated acidic glycoconjugates. A conspicuous increase in percentage of neutral glycoconjugates-containing goblet cells and a decrease of sialylated glycoconjugatescontaining goblet cells occurred as early as 5 minutes after the administration of the lower dose and were recorded 20 minutes post exposure, too. Five minutes post exposure, the higher dose evoked a decrease in percentage of both neutral-and sialylated glycoconjugates-containing goblet cells. Twenty minutes after the administration of the higher dose, percentage of all studied elements reached values similar to those found in the lower dose group. The cholinergic stimulation did not cause total disappearance of both neutral-and sialylated glycoconjugates-containing goblet cells. Only the reaction of goblet cells 5 minutes after the administration of the higher acetylcholine dose differed quantitatively from the reactions in remaining experimental groups. Airways' epithelium, cholinergic stimulation, glycoconjugates, lectin histochemistryIn our previous studies, we demonstrated that the goblet cells (GC) in the rabbit tracheal epithelium were overstimulated and damaged due to the intravenous (i.v.) administration of acetylcholine. An apocrine type of secretion and also chain exocytosis were found after the administration of both doses of acetylcholine. Twenty minutes after the administration of 0.5 mg of acetylcholine, the degeneration of GC evoked massive differentiation of new secretory elements (Konrádová et al. 1996ab). Similar reaction of GC was also described by Specian and Neutra (1980) in the tissue culture of intestinal GC, and by Newman et al. (1996) in the tracheal epithelium of guinea pigs. Tokuyama et al. (1990) andFung et al. (1992) confirmed the stimulating effect of vagal control to the secretion of airways' GC and submucosal glands. Ramnarine and Rogers (1994) stressed non-adrenergic, non-cholinergic (NANC) neural mechanisms, coworking with neurogenic acetylcholine as "the secretion fine tuning". A direct effect of acetylcholine on tracheal secretory elements was further confirmed as a dose-dependent in studies by Kamijo et al. (1993), Woods et al. (1996), andMatera et al. (1998). Miyata et al. (1998) doubted the acetylcholine role in controlling production of high molecular weight-glycoconjugates in spite of the study by Steel and Hanrahan (1997) on GC of the tracheal epithelium in hamsters.The presence of neutral and acidic sulphated and acidic sialylated glycoconjugates

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Section: Airways' Epithelium Cholinergic Stimulation Glycoconjugatementioning

confidence: 93%

Section: Airways' Epithelium Cholinergic Stimulation Glycoconjugatementioning

confidence: 99%

The Effect of Intravenously Administered acetylcholine on the Glycoconjugate Composition on Goblet Cells of the Tracheal Epithelium in Rabbits

Vajner¹,

Uhlı́k²,

Konrádová³

et al. 2000

Acta Vet. Brno

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“…Substâncias que aumentam a velocidade do transporte mucociliar têm grande aplicabilidade terapêutica (Miyata et al, 1998;Korolkovas, 1999) e todas as plantas que compõem as duas formulações fitoterápicas estudadas têm indicações terapêuticas para o tratamento das patologias do aparelho respiratório (Oliveira et al, 1993) e apresentam ação sobre este, razão pela qual podem ser denominadas antitussígenas periféricas que atuam nos receptores da tosse localizados no epitélio das vias respiratórias (Korolkovas, 1999).…”

Section: Discussionunclassified

Eficácia antitussígena de duas formulações fitoterápicas

Mello

2007

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliaram-se os efeitos antitussígenos-expectorantes de duas formulações fitoterápicas utilizando-se três modelos biológicos diferentes. Foram utilizados ratos Wistar no modelo da secreção das vias aéreas, cobaias no modelo de tosse induzido por ácido cítrico e codornas japonesas na determinação da velocidade de transporte mucociliar. Os animais foram distribuídos em grupos e tratados por via oral com as formulações, com doses equivalentes a 10 vezes a terapêutica recomendada, 9ml.

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“…Several reviews summarize the most clinically relevant mucoactive drugs used worldwide in the management of several acute and chronic respiratory diseases [116][117][118][119].…”

Section: Mucokineticsmentioning

confidence: 99%