Distribution of genotype/subtype and mutational spectra of the surface gene of hepatitis B virus circulating in Hanoi, Vietnam

Thủy, Lê Thị Thanh; Ryo, Haruko; Phung, Le Van; Furitsu, Katsumi; Nomura, Taisei

doi:10.1002/jmv.20337

Cited by 23 publications

(6 citation statements)

References 37 publications

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“…Unfortunately, the vaccination status of the mother from whom the HBV strain was obtained is unknown. Studies carried out in Morocco and in Vietnam reported mutations in these same positions: L109Q and G130A/N in unvaccinated patients [ 52 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of hepatitis B virus in Amerindian children and mothers from Amazonas State, Colombia

Jaramillo

Hoz²,

Porras³

et al. 2017

PLoS ONE

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BackgroundHepatitis B Virus (HBV) infection is a worldwide public health problem. In the 1980’s a highly effective and safe vaccine against HBV was developed, although breakthrough infection still occasionally occurs because of the emergence of escape mutants. The aim of this study was to identify HBV genotypes and escape mutants in children and their mothers in Amerindian communities of the Amazonas State, Southern Colombia.MethodsBlood specimens collected from children and mothers belonging to 37 Amerindian communities in Amazonas state, were screened for HBsAg and anti-HBc using ELISA. The partial region containing the S ORF was amplified by nested PCR, and amplicons were sequenced. The phylogenetic analysis was performed using the MEGA 5.05 software.ResultsForty-six children (46/1275, 3.6%) and one hundred and seventy-seven mothers (177/572, 30.9%) were tested positive for the anti-HBc serological marker. Among them, 190 samples were tested for viral genome detection; 8.3% (2/31) serum samples obtained from children and 3.1% (5/159) from mothers were positive for the ORF S PCR. The predominant HBV genotype in the study population was F, subgenotype F1b; in addition, subgenotype F1a and genotype A were also characterized. Two HBV escape mutants were identified, G145R, reported worldwide, and W156*; this stop codon was identified in a child with occult HBV infection. Other mutations were found, L109R and G130E, located in critical positions of the HBsAg sequence.ConclusionsThis study aimed to characterize the HBV genotype F, subgenotypes F1b and F1a, and genotype A in Amerindian communities and for the first time escape mutants in Colombia. Further investigations are necessary to elucidate the frequency and the epidemiological impact of the escape mutants in the country.

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Section: Discussionmentioning

confidence: 99%

Characterization of hepatitis B virus in Amerindian children and mothers from Amazonas State, Colombia

Jaramillo

Hoz²,

Porras³

et al. 2017

PLoS ONE

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“…Because it replicates through an RNA intermediate synthesized by reverse transcriptase, mutant viral genomes[5972] and quasi-species[71–77] are generated. This results in the production of viral mutants during naturally occurring infections.…”

Section: Escape Mutantsmentioning

confidence: 99%

“…[5976808187] Some mutations involve amino acid insertions into the a determinant[63788889] or the creation of stop codons. [7184] Other mutations eliminate the glycosylation site at residue 146[85] or create potential new glycosylation sites in the first loop of the MHR. [9091] However, not all mutations in the MHR lead to escape mutants.…”

Section: Escape Mutantsmentioning

confidence: 99%

Hepatitis B virus S gene escape mutants

Purdy¹

2007

Asian J Transfus Sci

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Hepatitis B virus (HBV) can be classified into nine immunological subtypes or eight genotypes. The most prevalent genotypes in Asia are genotypes B and C. HBV is transmitted parenteraly and can produce either asymptomatic or symptomatic disease. Although the consequences of acute hepatitis B can be severe, serious sequelae are associated with chronic infections. HBV seroprevalence ranges from intermediate (2%-7%) to high (≥8%) levels in Asia. Several strategies for the control and prevention of HBV infection have been found to be efficacious. They include vaccination and the administration of HBIG, interferon-a and nucleoside/nucleotide analogues. However, these procedures also apply selective pressures on HBV in infected individuals leading to the generation and accumulation of mutations in the S gene. Most of these mutations occur in the major hydrophilic region (MHR) of the S gene. These mutations create public health concerns as they can be responsible for reactivation of hepatitis B and occult hepatitis B infection. The inability to detect occult infections means that these individuals may become blood donors. This suggests that new strategies for donor evaluation and selection may need to be developed to protect the blood supply.

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“…Limited research has been conducted on molecular characterization and determination of virulence associated properties of HBV in Vietnam. Representative data on genotype and subgenotype analysis using whole genome sequence (WGS) [ 14 – 16 ], as well as information on the prevalence of virulence associated mutations in different genes, recombination and drug resistant mutations in treatment naïve patients are limited.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of hepatitis B virus in Vietnam

Bui

Tran²,

Nghiem

et al. 2017

BMC Infect Dis

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Background: Hepatitis B virus (HBV) infection is a major public health problem globally. HBV genotypes and subgenotypes influence disease transmission, progression, and treatment outcome. A study was conducted among treatment naive chronic HBV patients in southern Vietnam to determine the genotypes and subgenotypes of HBV. Methods: A prospective, exploratory study was conducted among treatment naïve chronic HBV patients attending at the Hospital for Tropical Diseases, in Ho Chi Minh City, Vietnam during 2012, 2014 and 2016. HBV DNA positive samples (systematically selected 2% of all treatment naïve chronic patients during 2012 and 2014, and 8% of all treatment naïve chronic patients during 2016) were subjected to whole genome sequencing (WGS) either by Sanger or Illumina sequencing. WGS was used to define genotype, sub-genotype, recombination, and the prevalence of drug resistance and virulence-associated mutations. Results: One hundred thirty five treatment naïve chronic HBV patients including 18 from 2012, 24 from 2014, and 93 from 2016 were enrolled. Of 135 sequenced viruses, 72.6% and 27.4% were genotypes B and C respectively. Among genotype B isolates, 87.8% and 12.2% were subgenotypes B4 and B2 respectively. A G1896A mutation in the precore gene was present in 30.6% of genotype B isolates. The genotype C isolates were all subgenotype C1 and 78.4% (29/37) of them had at least one basal core promoter (BCP) mutation. A1762T and G1764 T mutations and a double mutation (A1762T and G1764 T) in the BCP region were significantly more frequent in genotype C1 isolates (p < 0.001). Conclusion: HBV genotype B including subgenotype B4 is predominant in southern Vietnam. However, one fourth of the chronic HBV infections were caused by subgenotype C1.

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Distribution of genotype/subtype and mutational spectra of the surface gene of hepatitis B virus circulating in Hanoi, Vietnam

Cited by 23 publications

References 37 publications

Characterization of hepatitis B virus in Amerindian children and mothers from Amazonas State, Colombia

Characterization of hepatitis B virus in Amerindian children and mothers from Amazonas State, Colombia

Hepatitis B virus S gene escape mutants

Molecular characterization of hepatitis B virus in Vietnam

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