Distribution of Glycogen in the Rat Heart

Weisberg, Jerry; Rodbard, Simon

doi:10.1152/ajplegacy.1958.193.3.466

Cited by 17 publications

(3 citation statements)

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“…The normal bundle of His and the left bundle branch contain glycogen in higher proportions than the atrial and ventricular myocardium [10], and the atria contain glycogen in higher proportions than the ventricles [5,11] . When there is a generalized increase in glycogen, it may be difficult to deter- mine by histological techniques whether there are differences in the amount of glycogen increase in the atria, the ventricles, and the various parts of the conduction system .…”

Section: Discussionmentioning

confidence: 99%

The conduction system in Pompe's disease

et al. 1982

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We report our findings in the microscopic examination of the conduction system in four infants with glycogen storage disease, one of whom had adequate electrophysiologic studies. The electrophysiologic studies in the latter case showed P-A and A-H intervals at the lower limits of normal, but the H-V interval was just above the normal mean. This suggests that the rapid conduction was not localized in the anatomic counterpart of the H-V interval. The short P-R interval in the ECG may be related to the enlargement of cells, which may in turn be related to increased glycogen content. The relationship of glycogen per se to the speed of conduction is unknown. We found that the summit of the ventricular septum bulged, probably because of the generally increased cell size, and that the topography of the atrioventricular conducting system was different from normal. This is possibly related both to an increase in the cell sizes of the specialized conducting tissue itself and to deforming effects of this bulging summit of the ventricular septum. New microscopic details of the components of the conducting system are described in these cases.

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Section: Discussionmentioning

confidence: 99%

The conduction system in Pompe's disease

et al. 1982

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“…The glycogen values are expressed as percent of the concentration found in the initial sample removed at the time of completion of the isolated right ventricle preparation. A certain amount of variability in glycogen values was to be expected in these experiments because it was necessary for samples to be removed from different regions of the heart and it is known that there is a 30% variation in concentration from base to apex (3,5). To estimate the variability of die glycogen concentration in a time-course study, duplicate randomly located samples were taken at the end of a group of experiments.…”

Section: Methodsmentioning

confidence: 99%

Glycogen Concentration in Working and Nonworking Ventricles of Isolated Dog Hearts

Jedeikin¹,

Buckley

1967

Circulation Research

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Glycogen utilization in working and nonworking ventricles was studied at high (over 70 mg/100 ml) and low (27 to 61 mg/100 ml) arterial glucose concentrations and after insulin or epinephrine addition in 16 isolated ventricle preparations of dog hearts. Coronary perfusion and hemodynamic determinants of right ventricular work were controlled, and the left ventricle was kept unloaded. Time courses of change in ventricular glycogen concentration were determined during monitoring of heart rate, workload, arterial oxygen saturation, and coronary perfusion pressure. Epicardial samples for glycogen analysis were taken from each ventricle, and glucose uptake from circulating blood was determined. Glycogen loss was greater in working right than in nonworking left ventricles. In spontaneously fibrillating hearts, this difference was not observed, and there was greater glycogenolysis than during coordinated contraction. Insulin administration early in-experiments-led-to equivalent glycogen loss in working right and nonworking left ventricles. There was glycogen preservation in both ventricles of fibrillating hearts. Epinephrine augmented glycogen loss in fibrillating hearts; depletion was never complete. Myocardial glucose uptake, corrected for red cell glycolysis, was proportional to initial arterial glucose concentration. ADDITIONAL KEY WORDS myocardial glucose consumption fibrillationinsulin red cell glycolysis epinephrine • It has not yet been established whether the heart, when supplied with energy-yielding substrates in its coronary perfusate, utilizes glycogen for its energy requirements as well. We studied this question in a preparation that permitted simultaneous comparison between a working right ventricle that was pumping blood and a nonworking left ventricle that was merely contracting in the same dog heart. The metabolic pattern of glycogen was studied also in the fibrillating heart and, in another study (1), in the Langendorff preparation in which both ventricles were contracting.Glycogen concentration in each ventricle was determined in subepicardial tissue samples removed at intervals during experiments lasting 90 min. Throughout each experiment, the hearts were perfused via the coronary arteries with oxygenated whole blood or a partly synthetic medium at constant perfusion pressure, and the physiologic performance of the working right ventricle was monitored.

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“…The data from foetal lambs do not fit, but those experiments were executed at a higher temperature, under anaesthesia, and only the tip of the ventricle was taken for analysis. An increase in environmental temperature is known to reduce survival time (Reiss, 1931;Adolph, 1948;Miller & Miller, 1954), and the method of sampling under anaesthesia would lead to a high estimate of cardiac carbohydrate (Evans, 1934;Russell & Bloom, 1955;Timiras, Hill, Krum & Lis, 1958;Weisberg & Rodbard, 1958). The relationship between cardiac carbohydrate and resistance to anoxia at different ages may also apply to man.…”

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confidence: 99%