Distribution of Glyphosate in Chicken Organs and its Reduction by Humic Acid Supplementation

Abstract

Glyphosate (N-(phosphonomethyl) glycine) is a most popular herbicide in agricultural practices throughout the world. It is possible that glyphosate spread in the ecosystems can reach plants, animals. The present work was directed to investigate the glyphosate residue in different organs of broiler chickens using ELISA and to study the possibility of its neutralisation using humic acid, Chlorella vulgaris and Saccharomyces boulardii. Results showed that glyphosate residues could be detected in the animal feed and different organs as liver, spleen, lung, intestine, heart, muscles and kidney. Humic acid, Chlorella vulgaris and Saccharomyces boulardii showed neutralization of the antimicrobial effect of glyphosate in vitro. Also, feed supplementation of commercial broiler with humic acid (0.2%) leads to a significant decrease in the glyphosate content, i.e. by 53%, 28%, 44%, 50%, 56%, 16%, 63% and 0% in serum, liver, spleen, lung, gastro-intestinal tract, heart, muscles and kidney, respectively. There were no significant effects of humic acid on the production parameters. This enlightenment will help to overcome the negative effect of glyphosate residues on gastrointestinal microbiota and protect consumers from glyphosate residues in chicken meat.

The role of L-type amino acid transporters in the uptake of glyphosate across mammalian epithelial tissues

“…Due to the wide expression of amino acid transporters in a variety of organs, for example, LAT1 is expressed in brain, stomach, liver, intestine, heart, lung and testis and LAT2 is expressed in kidney, intestine, brain, liver, stomach, heart, lung and muscle (Del Amo et al, 2008), these organs are likely sites for increased glyphosate toxicity. A recent publication described the presence of measurable concentrations of glyphosate in the liver, spleen, intestine, kidneys, heart, and muscle tissue of chickens who consumed feed containing glyphosate (Shehata et al, 2014). Each of these organs and tissues is known to possess significant amino acid transporter activity which may explain the observed biodistribution pattern.…”

Detection of Glyphosate in Malformed Piglets

“…In conclusion, glyphosate could reach the animals through food Heart (N=8) 0.4 80 12.9 ± 29.8 1 Glyphosate was detected using ELISA [1] and data are expressed as µg/g.…”

“…One malformed piglet showed a swollen belly and fore gut and hind gut were not connected ( Figure 1). Different organs and tissues as lungs, liver, kidney, brain, gut wall and heart of malformed euthanized one-day-old Danish piglets were tested for glyphosate using ELISA [1]. Briefly, tissue samples were minced to small pieces (~0.25 cm).…”

Does Glyphosate Acting as a Glycine Analogue Contribute To ALS?

“…But this implies that a person who is athletic and physically fit is more likely to accumulate glyphosate in the muscle cells via transport through the L-type amino acid transporters, and it might help explain the observed increased risk to ALS among the physically fit [43,44] . Glyphosate residues have been detected in the muscles of chickens [45] and cows [29] .…”