Distribution of goblet cells and MUC5AC mRNA in the canine nictitating membrane

Umeda, Yasuyoshi; Nakamura, Sinji; Fujiki, Keiko; Toshida, Hiroshi; Saito, Akihiko; Murakami, Akira

doi:10.1016/j.exer.2010.08.020

Cited by 14 publications

(20 citation statements)

References 16 publications

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“…Earlier reports also revealed that the conjunctival fornix is very rich in goblet cells in dogs, 5 cats, 17 and horses, 6 but not in chinchillas 7 or guinea pigs. 8 Our finding that the anterior face of the third eyelid has high GCD was also reported in dogs 20 and cats. 19 Interestingly, a study conducted in humans revealed that the highest GCD is found on the plica semilunaris - a vestigial remnant of the nictitating membrane.…”

Section: Discussionsupporting

confidence: 77%

“…8 Our finding that the anterior surface of the third [Correction added on 11 April 2016, after first online publication: The headers "Dorsomedial" and "Ventrolateral" have been transposed above the subheader "Palpebral" to present the correct grouping.]. eyelid has high GCD was also reported in dogs 20 and cats. 19 Interestingly, a study conducted in humans revealed that the highest GCD is found on the plica semilunarisa vestigial remnant of the nictitating membrane.…”

Section: Discussionmentioning

confidence: 97%

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Goblet cell density and distribution in cats with clinically and histologically normal conjunctiva

Sebbag

Reilly

Eid

et al. 2016

Veterinary Ophthalmology

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Objective To evaluate goblet cell density (GCD) and distribution in cats without clinical evidence of ocular surface disease and without histologic evidence of conjunctival disease. Animals studied Fourteen domestic-shorthair cats euthanized for reasons unrelated to this study. Procedures Before euthanasia, cats were verified using slit-lamp biomicroscopy and fluorescein staining to be free of eyelid or ocular surface abnormalities. Immediately after euthanasia, bilateral conjunctival specimens including third eyelid (TEL) were collected, routinely processed, stained with Periodic acid-Schiff and hematoxylin and eosin. Thirteen conjunctival regions were identified. For each region, GCD was expressed as percentage of goblet cells/200 basal epithelial cells. Results Mean GCD ranged widely by region: anterior face of the TEL = 48.8%, fornicial regions = 47.0%, palpebral regions = 38.5%, bulbar regions = 19.6%, and posterior face of the TEL = 12.6%. The anterior surface of the TEL had significantly higher GCD than did the bulbar and the palpebral regions but not the fornicial regions. Bulbar conjunctiva had significantly lower GCD than did all other conjunctival regions except the posterior surface of the TEL. No significant difference was noted between GCD of male versus female cats, dorsal versus ventral regions or lateral versus medial regions. Conclusions Although conjunctival GCD ranged widely by region, the anterior surface of the TEL appears to be an excellent location for assessing conjunctival goblet cells in cats since this area has high GCD and is more readily accessible than are the palpebral, fornicial, or bulbar conjunctiva.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 97%

Goblet cell density and distribution in cats with clinically and histologically normal conjunctiva

Sebbag

Reilly

Eid

et al. 2016

Veterinary Ophthalmology

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“…The concentration of goblet cells was higher on the inner face of the third eyelid, as previously described . In histological sections, the goblet cells were strongly stained by both SPA and alcian blue, as previously reported, confirming the presence of mucus composed of neutral glycosylated substances (Figure A) and acids (Figure B) in the cells.…”

Section: Discussionsupporting

confidence: 87%

“…In humans and dogs, this mucin is present in the apical portion of the cell membrane of apical cells of the cornea and conjunctival epithelium, acting as a barrier to prevent the adherence of cells and pathogens. MUC5AC mucin, in turn, is secreted to the eye surface …”

Section: Discussionmentioning

confidence: 99%

Ultrastructural morphology of goblet cells of the conjunctiva of dogs

Araújo

Corrêa

Galera

2019

Veterinary Ophthalmology

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Objective: To describe the morphology of goblet cells of the eyelid conjunctiva in dogs using transmission and scanning electron microscopy. Animal studied: Ten dogs, both male and female of different breeds, with no ocular changes were examined (20 eyes). Procedures: Ten samples of conjunctiva were collected and processed for scanning and transmission electron microscopy (SEM and TEM), while another 10 samples were stained with Schiff's periodic stain (SPA) and alcian blue, pH 2.5, and analyzed using light microscopy. Results: Scanning electron microscopy revealed several points of mucus extrusion in the free apical portion of the goblet cells as well as a wide distribution of lymphoid follicles and macrophages intermingling with the microvilli of palpebral epithelium cells. TEM revealed normal goblet cells that were predominantly oval with wide cytoplasm of different diameters, and large vesicles with heterogeneous granules and free edges, suggesting the release of mucus content onto the conjunctival surface.Cytoplasmic organelles, such as the Golgi apparatus, endoplasmic reticulum, and a high number of mitochondria were also observed. All the samples were positive for SPA and alcian blue staining. Conclusion: This is the first study to evaluate the goblet cells of the eyelid conjunctiva in healthy dogs using electron microscopy techniques. These results are useful for comparing the palpebral conjunctiva of dogs without ocular changes to palpebral conjunctiva of dogs and other species with ocular changes. K E Y W O R D Sdogs, electron microscopy, goblet cells, keratoconjunctivitis sicca, ocular surface How to cite this article: Araújo RLS, Corrêa JR, Galera PD. Ultrastructural morphology of goblet cells of the conjunctiva of dogs. Vet Ophthalmol.

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“…In dogs, goblet cells are predominantly located on the palpebral surface of the nictitating membrane and on the medial conjunctival fornix. 37,38 With regard to tear production, only STT1 values were used as criteria for inclusion of dogs into the study. TBUT was not considered because of the high variability of values in the control group.…”

Section: Discussionmentioning

confidence: 99%